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Myosin heavy chain pattern in the Akhal-Teke horses

  • K. Leisson (a1), K. Alev (a2), P. Kaasik (a2), Ü. Jaakma (a1) and T. Seene (a2)...

This study investigates the myosin heavy chain (MyHC) isoform composition in the gluteus medius muscle of the Akhal-Teke horses using SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). Fifteen horses aged between 1.5 and 23.5 years were used in this study and divided into three age groups: 1.5 to 4 (n = 6), 9 to 13 (n = 5) and 18.5 to 23.5 years (n = 4). The average content of the MyHC I isoform was 11.72 ± 1.07% (variation between individuals: 7.09% to 20.14%). The relative content of the MyHC IIa and IIx isoforms was subsequently 38.20 ± 1.46% (30.73% to 48.78%) and 50.07 ± 1.10% (43.8% to 56.78%) from the total MyHC. The MyHC pattern in the skeletal muscles of the Akhal-Teke horses shows that the muscles of these horses have a high capacity both for endurance and speed.

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Barrey, E, Valette, JP, Jouglin, M, Blouin, C, Langlois, B 1999. Heritability of percentage of fast myosin heavy chains in skeletal muscles and relationship with performance. Equine Veterinary Journal Supplements 30, 289292.
D'Albis, A, Pantaloni, C, Bechet, JJ 1979. An electrophoretic study of native myosin isozymes and their subunit content. European Journal of Biochemistry 99, 261272.
Dingboom, EG, van Oudheusden, H, Eizema, K, Weijs, WA 2002. Changes in fiber type composition of gluteus medius and semitendinosus muscles of Dutch Warmblood foals and the effect of exercise during the first year postpartum. Equine Veterinary Journal 34, 177183.
Gondim, FJ, Modolo, LV, Campos, GE, Salgado, I 2005. Neuronal nitric oxide synthase is heterogeneously distributed in equine myofibers and highly expressed in endurance trained horses. Canadian Journal of Veterinary Research 69, 4652.
Gunn, HM 1995. Relative increase in areas of muscle fiber types in horses during growth. Equine Veterinary Journal 27, 209213.
Hendricks, BL 1995. International Encyclopedia of horse breeds. University of Oklahoma Press, Oklahoma City, OK, USA.
Kawai, M, Minami, Y, Sayama, Y, Kuwano, A, Hiraga, A, Miyata, H 2009. Muscle fiber population and biochemical properties of whole body muscles in Thoroughbred horses. The anatomical record: advances in integrative anatomy and evolutionary biology 292, 16631669.
Kim, JS, Hinchcliff, KW, Yamaguchi, M, Beard, LA, Markert, CD, Devor, ST 2005. Age-related changes in metabolic properties of equine skeletal muscle associated with muscle plasticity. The Veterinary Journal 169, 397403.
Kohn, TA, Myburgh, KH 2006. Electrophoretic separation of human skeletal muscle myosin heavy chain isoforms: importance of reducing agents. The Journal of Physiological Sciences 56, 355360.
Kuznecova, Y 2005. K 70-letiyu konnogo perehoda Ashabad – Moskva. Ahal-Teke Inform, 7883.
Ledwith, A, McGowan, CM 2004. Muscle biopsy: a routine diagnostic procedure. Equine Veterinary Education 16, 6267.
Lehnhard, RA, McKeever, KH, Kearns, C, Beekley, MD 2004. Myosin heavy chain profiles and body composition are different in old versus young Standardbred mares. The Veterinary Journal 167, 5966.
Leisson, K, Jaakma, Ü, Seene, T 2008. Adaptation of equine locomotor muscle fiber types to endurance and intensive high speed training. Journal of Equine Veterinary Science 28, 395401.
Lopez, JL, Galisteo, AM, Serrano, AL, Morales, JL 1993. Influencia del semental sobre tipo y composicion de fibra muscular en caballos arabes y andaluces en diferentes estados de desarrollo postnatal. Agro-Ciencia 9, 4348.
Lopez-Rivero, JL, Morales-Lopez, JL, Galisteo, AM, Aguera, E 1991. Muscle fiber type composition in untrained and endurance-trained Andalusian and Arab horses. Equine Veterinary Journal 23, 9193.
Majarina, S 2008. Materinskii kapital. Ahal-Teke Inform 176180.
Oakley, B, Kirsch, D, Morris, N 1980. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Analytical Biochemistry 105, 361363.
Priskin, K, Szabo, K, Tömöry, G, Bogacsi-Szabo, E, Csanyi, B, Eördögh, R, Downes, CS, Rasko, I 2010. Mitochondrial sequence variation in ancient horses from the Carpathian Basin and possible modern relatives. Genetica 138, 211218.
Rietbroek, NJ, Dingboom, EG, Everts, ME 2006. Muscle characteristics of Dutch warmblood foals with different genetic background at ages 6 and 12 months. Equine Veterinary Journal Supplements 36, 326329.
Rivero, JLL 2007. A scientific background for skeletal muscle conditioning in equine practice. Journal of Veterinary Medicine 54, 321332.
Rivero, JLL, Diz, AM 1992. Skeletal muscle histochemistry of Andalusian horses: a comparative study with other breeds. Archiva Zootechnica 41, 505512.
Rivero, JLL, Henckel, P 1996. Muscle biopsy index for discriminating between endurance horses with different performance records. Research in Veterinary Science 61, 4954.
Rivero, JLL, Barrey, E 2001. Heritabilities and genetic and phenotypic parameters for gluteus medius muscle fibre type composition, fibre size and capillaries in purebred Spanish horses. Livestock Production Science 72, 233241.
Rivero, JLL, Talmadge, RJ, Edgerton, VR 1996a. Myosin heavy chain isoforms in adult equine skeletal muscle: and immunochemical and electrophoretic study. The Anatomical Record 246, 185194.
Rivero, JLL, Valera, M, Serrano, AL, Vinuesa, M 1996b. Variability of muscle fiber type composition in a number of genealogical bloodlines in Arabian and Andalusian horses. Pferdeheilkunde 12, 661665.
Rivero, JLL, Galisteo, AM, Aguera, E, Miro, F 1993a. Skeletal muscle histochemistry in male and female Andalusian and Arabian horses of different ages. Research in Veterinary Science 54, 160169.
Rivero, JLL, Serrano, AL, Henckel, P, Aguera, E 1993b. Muscle fiber type composition and fiber size in successfully and unsuccessfully endurance-raced horses. Journal of Applied Physiology 75, 17581766.
Rivero, JLL, Ruz, A, Marti-Korff, S, Estepa, JC, Aguilera-Tejero, E, Werkmann, J, Sobotta, M, Lindner, A 2007. Effects of intensity and duration of exercise on muscular responses to training of thoroughbred horses. Journal of Applied Physiology 102, 18711882.
Roneus, M 1993. Muscle characteristics in Standardbreds of different ages and sexes. Equine Veterinary Journal 25, 143146.
Roneus, M, Lindholm, A, Asheim, A 1991. Muscle characteristics in Thoroughbreds of different ages and sexes. Equine Veterinary Journal 23, 207210.
Roneus, M, Essen-Gustavsson, B, Lindholm, A, Persson, SG 1992. Skeletal muscle characteristics in young trained and untrained Standardbred trotters. Equine Veterinary Journal 24, 292294.
Ryabova, TN 2005. Gosudarstvennaya kniga plemennyh loshadei cistokrovnoi ahaltekinskoi porody, tom X. Vserossiiskii nauchno-issledovatelskii institut konevodstva, Divovo, Russia.
Seene, T, Kaasik, P, Umnova, M 2009. Structural rearrangements in contractile apparatus and resulting skeletal muscle remodelling: effect of exercise training. Journal of Sports Medicine and Physical Fitness 49, 410423.
Seene, T, Umnova, M, Kaasik, P, Alev, K, Pehme, A 2008. Overtraining injuries in athletic population. In Skeletal muscle damage and repair (ed. PM Tiidus), pp. 173184. Human Kinetics, Champaign, IL, USA.
Serrano, AL, Quiroz-Rothe, E, Rivero, JLL 2000. Early and long-term changes of equine skeletal muscle in response to endurance training and detraining. Pflugers Archive European Journal of Physiology 441, 263274.
Snow, DH, Guy, PS 1980. Muscle fibre type composition of a number of limb muscles in different types of horse. Research in Veterinary Science 28, 137144.
Szontagh, A, Ban, B, Bodo, I, Cothran, EG, Hecker, W, Jozsa, Cs, Major, A 2005. Genetic diversity of the Akhal Teke horse in Turkmenistan based on microsatellite analysis. In Conservation genetics of endangered horse breeds (ed. I Bodo, L Alderson and B Langolis), pp. 123128. Wageningen Academic Publishers, Wageningen, The Netherlands.
Talmadge, RJ, Roy, RR 1993. Electrophoretic separation of rat skeletal muscle heavy-chain isoforms. Journal of Applied Physiology 75, 23372340.
Tikhonov, VN, Cothran, EG, Kniazev, SP 1998. Population genetic parameters of aboriginal Yakut horses as related to modern breeds of the domestic horse Equus caballus L. Genetica 34, 796809.
Votion, DM, Navet, R, Lacombe, VA, Sluse, F, Essen-Gustavsson, B, Hinchcliff, KW, Rivero, JLL, Serteyn, D, Valberg, S 2007. Muscle energetics in exercising horses. Equine and Comparative Exercise Physiology 4, 105118.
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