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The origin of the Tibetan Mastiff and species identification of Canis based on mitochondrial cytochrome c oxidase subunit I (COI) gene and COI barcoding

  • Y. Li (a1), X. Zhao (a2), Z. Pan (a1), Z. Xie (a1), H. Liu (a1), Y. Xu (a1) and Q. Li (a1)...
Abstract

DNA barcoding is an effective technique to identify species and analyze phylogenesis and evolution. However, research on and application of DNA barcoding in Canis have not been carried out. In this study, we analyzed two species of Canis, Canis lupus (n = 115) and Canis latrans (n = 4), using the cytochrome c oxidase subunit I (COI) gene (1545 bp) and COI barcoding (648 bp DNA sequence of the COI gene). The results showed that the COI gene, as the moderate variant sequence, applied to the analysis of the phylogenesis of Canis members, and COI barcoding applied to species identification of Canis members. Phylogenetic trees and networks showed that domestic dogs had four maternal origins (A to D) and that the Tibetan Mastiff originated from Clade A; this result supports the theory of an East Asian origin of domestic dogs. Clustering analysis and networking revealed the presence of a closer relative between the Tibetan Mastiff and the Old English sheepdog, Newfoundland, Rottweiler and Saint Bernard, which confirms that many well-known large breed dogs in the world, such as the Old English sheepdog, may have the same blood lineage as that of the Tibetan Mastiff.

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      The origin of the Tibetan Mastiff and species identification of Canis based on mitochondrial cytochrome c oxidase subunit I (COI) gene and COI barcoding
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      The origin of the Tibetan Mastiff and species identification of Canis based on mitochondrial cytochrome c oxidase subunit I (COI) gene and COI barcoding
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Copyright
Corresponding author
E-mail: liqifa@njau.edu.cn
References
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Björnerfeldt S, Webster MT, Vilà C 2006. Relaxation of selective constraint on dog mitochondrial DNA following domestication. Genome Research 16, 990994.
Derycke S, Vanaverbeke J, Rigaux A, Backeljau T, Moens T 2010. Exploring the use of cytochrome oxidase c subunit 1 (COI) for DNA barcoding of free-living marine nematodes. PLoS One 5, e13716.
Hebert PDN, Cywinska A, Ball SL, de Waard JR 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences 270, 313321.
Hebert PDN, Stoeckle MY, Zemlak TS, Francis CM 2004. Identification of birds through DNA barcodes. PLoS Biology 2, e312.
Janzen DH, Hajibabaei M, Burns JM, Hallwachs W, Remigio E, Hebert PDN 2005. Wedding biodiversity inventory of a large and complex lepidoptera fauna with DNA barcoding. Philosophical Transactions of the Royal Society B – Biological Sciences 360, 18351845.
Kress WJ, Erickson DL 2008. DNA barcodes: genes, genomics, and bioinformatics. Proceedings of the National Academy of Sciences of the United States of America 105, 27612762.
Kumar S, Dudley J, Nei M, Tamura K 2008. MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Briefings in Bioinformatics 9, 299306.
Li Y, Li Q, Zhao X, Xie Z, Xu Y 2011. Complete sequence of the Tibetan Mastiff mitochondrial genome and its phylogenetic relationship with other Canids (Canis, Canidae). Animal 5, 1825.
Li Q, Liu Z, Li Y, Zhao X, Dong L, Pan Z, Sun Y, Li N, Xu Y, Xie Z 2008. Origin and phylogenetic analysis of Tibetan Mastiff based on the mitochondrial DNA sequence. Journal of Genetics and Genomics 35, 335340.
Librado P, Rozas J 2009. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25, 14511452.
Luo LY 2003. Origin of modern domestic dogs: a mitochondrial DNA perspective. Yanglin: Northwest Sci-tech University of Agriculture and Forestry, pp. 1769.
Polzin T, Daneschmand SV 2003. On Steiner trees and minimum spanning trees in hypergraphs. Operations Research Letters 31, 1220.
Ren DR, Yang QY, Ye JH, Xu L, Zhao HA, Wu XP 2009. Strong heterozygote deficit in Tibetan Mastiff of China based on microsatellite loci. Animal 3, 12131215.
Savolainen P, Zhang YP, Luo J, Lundeberg J, Leitner T 2002. Genetic evidence for an East Asian origin of domestic dogs. Science 298, 16101613.
Vilà C, Savolainen P, Maldonado JE, Amorim IR, Rice JE, Honeycutt RL, Crandall KA, Lundeberg J, Wayne RK 1997. Multiple and ancient origins of the domestic dog. Science 276, 16871689.
Yassin A, Markow TA, Narechania A, O'Grady PM, DeSalle R 2010. The genus Drosophila as a model for testing tree- and character-based methods of species identification using DNA barcoding. Molecular Phylogenetics and Evolution 57, 509517.
Yoo HS, Eah JY, Kim JS, Kim YJ, Min MS, Paek WK, Lee H, Kim CB 2006. DNA barcoding Korean birds. Mol Cells 22, 323327.
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animal
  • ISSN: 1751-7311
  • EISSN: 1751-732X
  • URL: /core/journals/animal
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