Skip to main content
×
×
Home

A preliminary study on the quality and safety of milk in donkeys positive for Toxoplasma gondii

  • M. Martini (a1), I. Altomonte (a1), F. Mancianti (a1), S. Nardoni (a1), L. Mugnaini (a1) and F. Salari (a1)...
Abstract

Toxoplasmosis is one of the five parasitic diseases considered as a priority for public health action. The consumption of raw milk products represents a possible risk, in particular for certain categories of people. The aim of this study was to evaluate the possible effects of Toxoplasma gondii on milk yield and quality in sero-positive animals with parasitemia. Eighteen healthy lactating Amiata jennies, between 90 and 180 days were included in the study. Four donkeys scored positive for immunofluorescent antibody test (IFAT), and each IFAT positive donkey presented parasitic DNA both in the blood and milk. No significant differences were found between milk yield in PCR-positive donkeys compared with the negative cases, however the former tended to have a greater production. Milk quality in the positive donkeys showed a significantly lower percentage of casein (0.72% v. 0.81%) and ash (0.32% v. 0.37%). Positive cases had a highly significant larger average diameter of globules (2.35 µm) and fewer globules/ml (2.39×108). Somatic cell and bacterial counts were normal and in agreement with the literature. Toxoplasma gondii did not seem to present clinical forms in lactating jennies. Further in vivo studies are needed to further assess the risk of T. gondii transmission through donkey milk, together with the impact of different stages of infection on milk quality.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      A preliminary study on the quality and safety of milk in donkeys positive for Toxoplasma gondii
      Available formats
      ×
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      A preliminary study on the quality and safety of milk in donkeys positive for Toxoplasma gondii
      Available formats
      ×
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      A preliminary study on the quality and safety of milk in donkeys positive for Toxoplasma gondii
      Available formats
      ×
Copyright
Corresponding author
E-mail: mina.martini@unipi.it
References
Hide All
Association of Official Analytical Chemists (AOAC) 1995. Official methods of analysis, 16th edition. AOAC, Arlington, VA, USA.
Bendixen, E, Danielsen, M, Hollung, K, Gianazza, E and Miller, I 2011. Farm animal proteomics – a review. Journal of Proteomics 74, 282293.
Camossi, LG, Greca-Júnior, H, Corrêa, APFL, Richini-Pereira, VB, Silva, RC, Da Silva, AV and Langoni, H 2011. Detection of Toxoplasma gondii DNA in the milk of naturally infected ewes. Veterinary Parasitology 177, 256261.
Chardès, T, Bourguin, I, Mevelec, MN, Dubremetz, JF and Bout, D 1990. Antibody responses to Toxoplasma gondii in sera, intestinal secretions, and milk from orally infected mice and characterization of target antigens. Infection and Immunity 58, 12401246.
Dehkordi, FS, Rahimi, E and Abdizadeh, R 2013. Detection of Toxoplasma gondii in raw caprine, ovine, buffalo, bovine, and camel milk using cell cultivation, cat bioassay, capture ELISA, and PCR methods in Iran. Foodborne Pathogens and Disease 10, 120125.
Dubey, JP 2010. Toxoplasmosis of animals and humans, 2nd edition. CRC Press, Boca Raton, FL, USA.
Evers, JM 2004. The milk fat globule membrane – compositional and structural changes post secretion by the mammary secretory cell. International Dairy Journal 14, 661674.
Faye, B and Konuspayeva, G 2012. The sustainability challenge to the dairy sector – the growing importance of non-cattle milk production worldwide. International Dairy Journal 24, 5056.
JMP 2002. User’s guide (version 5.0). SAS Institute Inc., Cary, NC, USA.
Jones, CD, Okhravi, N, Adamson, P, Tasker, S and Lightman, S 2000. Comparison of PCR detection methods for B1, P30, and 18S rDNA genes of T. gondii in aqueous humor. Investigative Ophthalmology and Visual Science 41, 634644.
Mancianti, F, Nardoni, S, D’Ascenzi, C, Pedonese, F, Mugnaini, L, Franco, F and Papini, R 2013. Seroprevalence, detection of DNA in blood and milk, and genotyping of Toxoplasma gondii in a goat population in Italy. Biomed Research International 2013, 16.
Mancianti, F, Nardoni, S, Papini, R, Mugnaini, L, Martini, M, Altomonte, I, Salari, F, D’Ascenzi, C and Dubey, JP 2014. Detection and genotyping of Toxoplasma gondii DNA in the blood and milk of naturally infected donkeys (Equus asinus). Parasites & Vectors 7, 165.
Martini, M, Salari, F and Altomonte, I 2013. The macrostructure of milk lipids: the fat globules. Critical Review in Food Science and Nutrition (doi:10.1080/10408398.2012.758626. Published online by Taylor & Francis 14 June 2013.
Montoya, JG and Liesenfeld, O 2004. Toxoplasmosis. Lancet 363, 19651976.
Pilla, R, Daprà, V, Zecconi, A and Piccinini, R 2010. Hygienic and health characteristics of donkey milk during a follow-up study. Journal of Dairy Research 77, 16.
Polidori, P, Beghelli, D, Mariani, P and Vincenzetti, S 2009. Donkey milk production: state of the art. Italian Journal of Animal Science 8, 677683.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

animal
  • ISSN: 1751-7311
  • EISSN: 1751-732X
  • URL: /core/journals/animal
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed