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British Journal of Nutrition British Journal of Nutrition

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Dietary berberine regulates lipid metabolism in muscle and liver of black sea bream (Acanthopagrus schlegelii) fed normal or high-lipid diets

Published online by Cambridge University Press:  28 July 2020

Lei Wang Open the ORCID record for Lei Wang[Opens in a new window] ,
Bingying Xu ,
Gladstone Sagada ,
Wing-Keong Ng ,
Kai Chen ,
Jinzhi Zhang  and
Qingjun Shao
Lei Wang
Affiliation:
College of Animal Sciences, Zhejiang University, Hangzhou 310058, People’s Republic of China
Bingying Xu
Affiliation:
College of Animal Sciences, Zhejiang University, Hangzhou 310058, People’s Republic of China
Gladstone Sagada
Affiliation:
College of Animal Sciences, Zhejiang University, Hangzhou 310058, People’s Republic of China
Wing-Keong Ng
Affiliation:
Fish Nutrition Laboratory, School of Biological Sciences, Universiti Sains Malaysia, Penang 11800, Malaysia
Kai Chen
Affiliation:
College of Animal Sciences, Zhejiang University, Hangzhou 310058, People’s Republic of China
Jinzhi Zhang*
Affiliation:
College of Animal Sciences, Zhejiang University, Hangzhou 310058, People’s Republic of China
Qingjun Shao*
Affiliation:
College of Animal Sciences, Zhejiang University, Hangzhou 310058, People’s Republic of China Ocean Academy, Zhejiang University, Zhoushan 316021, People’s Republic of China
*
* Corresponding authors: Jinzhi Zhang, email zhangjzs@zju.edu.cn; Qing-jun Shao, email qjshao@zju.edu.cn
* Corresponding authors: Jinzhi Zhang, email zhangjzs@zju.edu.cn; Qing-jun Shao, email qjshao@zju.edu.cn
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Abstract

The present study investigated the influence of berberine (BBR) supplementation in normal and high-lipid (HL) diets on lipid metabolism and accumulation in black sea bream (Acanthopagrus schlegelii). BBR was supplemented at 50 mg/kg to control (Con, 11·1 % crude lipid) and high-lipid (HL, 20·2 % crude lipid) diets and named as ConB and HLB, respectively. After the 8-week feeding trial, fish body length and specific growth rate were significantly reduced by HL diets (P < 0·05). Muscle and whole-body crude lipid contents were significantly influenced by both BBR supplementation and dietary lipid level. Fish fed the HLB diet had significantly lower serum TAG, LDL-cholesterol contents and alanine aminotransferase activity compared with the HL group. The HL group presented vast lipid accumulation in the liver, and hypertrophied hepatocytes along with large lipid droplets, and translocation of nuclear to the cell periphery. These abnormalities in black sea bream were alleviated in the HLB group. BBR supplementation in the HL diet significantly down-regulated the hepatic expression levels of acetyl-CoA carboxylase α, sterol regulatory element-binding protein-1, 6-phosphogluconate dehydrogenase, glucose 6-phosphate dehydrogenase and pparγ, whereas the lipoprotein lipase, hormone-sensitive lipase and carnitine palmitoyltransferase 1a expression levels were significantly up-regulated. However, the expression levels of these genes showed opposite trends in muscle (except for pparγ). In conclusion, dietary BBR supplementation in the HL diet reduced hepatic lipid accumulation by down-regulating lipogenesis gene expression and up-regulating lipolysis gene expression, and it increased muscle lipid contents with opposite trends of the mechanism observed in the liver.

Keywords

Acanthopagrus schlegeliiBerberineLipid metabolismGrowth performance
Type
Full Papers
Information
British Journal of Nutrition , Volume 125 , Issue 5 , 14 March 2021 , pp. 481 - 493
Copyright
© The Author(s), 2020. Published by Cambridge University Press on behalf of The Nutrition Society

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References

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Dietary berberine regulates lipid metabolism in muscle and liver of black sea bream (Acanthopagrus schlegelii) fed normal or high-lipid diets
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