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Accumulation and apparent oxidation of cis,trans-18 : 2 isomers relative to linoleic acid in rats

Published online by Cambridge University Press:  09 March 2007

Zhen-Yu Chen ,
Kwok Yiu Kwan  and
Yu Huang
Zhen-Yu Chen*
Affiliation:
Departments of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
Kwok Yiu Kwan
Affiliation:
Departments of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
Yu Huang
Affiliation:
Departments of PhysiologyThe Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
*
*Corresponding author: Dr Zhen-Yu Chen, fax +852 2603 5123, email zhenyuchen@cuhk.edu.hk
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Abstract

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Dietary cis,trans-18 : 2 isomers impair desaturation and elongation of linoleic acid (Δ9cis,12cis-18 : 2), but little is known of their proportional partitioning between accumulation and oxidation. The present study was therefore designed to assess the accumulation and apparent oxidation of cis,trans-18 : 2 isomers compared with that of trans-18 : 1 isomers and Δ9cis,12cis-18 : 2 in rats. Accumulation is defined as whole-body increase in a fatty acid during a given period (i.e. final body content-initial body content). The apparent oxidation (disappearance) is defined as whole-body utilization of a fatty acid relative to its intake for a given period (intake-excretion-accumulation-longer-chain products)/intake×100). The animals were fed on a diet containing 15 % (w/w) partially hydrogenated rapeseed oil with 1·72 % energy as cis,trans-18 : 2 isomers and varying amounts of Δ9cis,12cis-18 : 2. The apparent oxidation of total cis,trans-18 : 2 isomers (72–76 % dietary intake) was greater than that of Δ9cis,12cis-18 : 2 (38–51 % dietary intake) but it was similar to that of total trans-18 : 1 isomers (78–82 % dietary intake). Among the four isomers, the apparent oxidation of Δ9trans,12trans-18 : 2 was greater than that of the other isomers including Δ9trans,12cis-18 : 2, Δ9cis,12trans-18 : 2 and Δ9cis,13trans-18 : 2. Accumulation of Δ5cis,8cis,11cis,15trans-20 : 4 and Δ5cis,8cis,11cis,14trans-20 : 4 derived from chain-elongation and desaturation of Δ9cis,13trans-18 : 2 and Δ9cis,12trans-18 : 2 was decreased when the dietary Δ9cis,12cis-18 : 2 supply was increased.

Keywords

Linoleic acidOxidationcis,trans-18 : 2 isomersPartially hydrogenated rapeseed oiltrans-Fatty acidstrans-Linoleic acids
Type
Research Article
Information
British Journal of Nutrition , Volume 86 , Issue 2 , August 2001 , pp. 249 - 255
Copyright
Copyright © The Nutrition Society 2001

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