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Anticarcinogenic effect of probiotic fermented milk and chlorophyllin on aflatoxin-B1-induced liver carcinogenesis in rats

  • M. Kumar (a1), V. Verma (a2), R. Nagpal (a3), A. Kumar (a4), P. V. Behare (a5), B. Singh (a6) and P. K. Aggarwal (a1)...
Abstract

The present investigation was carried out to evaluate the hepatoprotective effect of probiotic fermented milk (FM) containing Lactobacillus rhamnosus GG and Lactobacillus casei strain Shirota, alone as well as in combination with chlorophyllin (CHL) as an antioxidant agent in male Wistar rats administered aflatoxin-B1 (AFB1). AFB1 was injected intraperitoneally at the rate of 450 μg/kg body weight per animal twice a week for 6 weeks, maintaining an equal time interval between the two consecutive AFB1 administrations. A total of 125 male Wistar rats were randomly allocated to five groups, each group having twenty-five animals. Group I was offered FM containing L. rhamnosus GG and L. casei strain Shirota. Group II was administered AFB1 and served as the control group; group III was administered FM-AFB1, in which besides administering AFB1, FM was also offered. Group IV was offered CHL and AFB1, and group V was offered both FM and CHL along with AFB1. The rats were euthanised at the 15th and 25th week of the experiment and examined for the biochemical and hepatopathological profile. A significant reduction in thiobarbituric acid-reactive substances (TBARS) was observed in the FM–CHL–AFB1 group compared with the AFB1 control group. FM alone or in combination with CHL was found to show a significant (P < 0·05) hepatoprotective effect by lowering the levels of TBARS and by enhancing the activities of antioxidant enzymes such as glutathione peroxidase, superoxide dismutase, catalase and glutathione-S-transferase, indicating that probiotic FM alone or in combination with CHL possesses a potent protective effect against AFB1-induced hepatic damage.

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*Corresponding author: M. Kumar, email manoj15micro@yahoo.co.in
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6 C Haskard , C Binnion & J Ahokas (2000) Factors affecting the sequestration of aflatoxin by Lactobacillus rhamnosus strain GG. Chem Biol Interact 128, 3949.

10 H Annuk , J Shchepetova , T Kullisaar , (2003) Characterization of intestinal lactobacilli as putative probiotic candidates. J Appl Microbiol 94, 403412.

13 E Tavan , C Cayuela , JM Antoine , (2002) Effects of dairy products on heterocyclic aromatic amine-induced rat colon carcinogenesis. Carcinogenesis 23, 477483.

17 D Sharma , SS Kumar & KB Sainis (2007) Antiapoptotic and immunomodulatory effects of chlorophyllin. Mol Immunol. 44, 347359.

21 M Uchiyama & M Mahira (1978) Determination of malonaldehyde precursor in tissue by thiobarbituric acid test. Anal Biochem 86, 271278.

22 GL Ellman (1959) Tissue sulphydryl groups. Arch Biochem Biophys 82, 7077.

23 AL Tapple (1978) Glutathione peroxidase and hydroperoxidase. Methods Enzymol 52, 506513.

28 HS Kim , DB Hausman , MM Comton , (2000) Induction of apoptosis by all-trans-retinoic acid and C2-ceramide treatment in rat stromal-vascular cultures. Biochem Biophys Res Commun 270, 7680.

31 AK Chaudhary , M Nokubo , LJ Marnett , (1994) Analysis of the malondialdehyde-2′-deoxyguanosine adduct in rat liver DNA by gas chromatography/electron capture negative chemical ionization mass spectrometry. Biol Mass Spectrom 23, 457464.

38 HM Shen , CY Shi , HP Lea , (1994) Aflatoxin B1-induced lipid peroxidation in rat liver. Toxicol Appl Pharmacol 127, 145150.

39 H Abdelali , P Cassand , V Soussotte , (1995) Effect of dairy products on initiation of precursor lesions of colon cancer in rats. Nutr Cancer 24, 121123.

40 I El-Gibaly , AM Meki & SK Abdel-Ghaffar (2003) Novel B-melatonin loaded chitosan macrocapsules: in vitro characterization and antiapoptosis efficacy for aflatoxin B1 induced apoptosis in rat liver. Int J Pharmacol 260, 2254.

41 C Jubert , J Mata , G Bench , (2009) Effects of chlorophyll and chlorophyllin on low-dose aflatoxin B(1) pharmacokinetics in human volunteers. Cancer Prev Res (Phila) 2, 10151022.

42 Y Zhang , L Guan , X Wang , (2008) Protection of chlorophyllin against oxidative damage by inducing HO-1 and NQO1 expression mediated by PI3K/Akt and Nrf2. Free Radic Res 42, 362371.

43 E Kot & A Bezkorovainy (2000) The binding of H2O2-generated Fe (III) to Bifidobacterium thermophilum depends on the method of its production. J Food Sci 65, 10281032.

45 L Gate , J Paul , BA Nguyen , (1999) Oxidative stress induced in pathologies: the role of antioxidants. Biomed Pharmacother 53, 169180.

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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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