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Antioxidative probiotic fermented goats' milk decreases oxidative stress-mediated atherogenicity in human subjects

  • Tiiu Kullisaar (a1), Epp Songisepp (a2), Marika Mikelsaar (a2), Kersti Zilmer (a1), Tiiu Vihalemm (a1) and Mihkel Zilmer (a1)...
Abstract

The increasing interest in a healthy diet is stimulating innovative development of novel scientific products in the food industry. The viable lactic acid bacteria in fermented milk products, such as yoghurt, have been associated with increased lactose tolerance, a well-balanced intestinal microflora, antimicrobial activity, stimulation of the immune system and antitumoural, anticholesterolaemic and antioxidative properties in human subjects. Recently, we have studied a human Lactobacillus spp. strain that possesses antioxidative activity. The aim of the present pilot study was to develop goats' milk fermented with the human antioxidative lactobacilli strain, Lactobacillus fermentum ME-3, and to test the effect of the fermented probiotic goats' milk on oxidative stress markers (including markers for atherosclerosis) in human blood and urine and on the gut microflora. Twenty-one healthy subjects were assigned to two treatment groups: goats' milk group and fermented goats' milk group (150 g/d) for a period of 21 d. Consumption of fermented goats' milk improved anti-atherogenicity in healthy subjects: it prolonged resistance of the lipoprotein fraction to oxidation, lowered levels of peroxidized lipoproteins, oxidized LDL, 8-isoprostanes and glutathione redox ratio, and enhanced total antioxidative activity. The consumption of fermented goats' milk also altered both the prevalence and proportion of lactic acid bacteria species in the gut microflora of the subjects. We conclude that the goats' milk fermented with our special antioxidative lactobacilli strain Lactobacillus fermentum ME-3 exhibits anti-atherogenic effects.

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Corresponding author
*Corresponding author: Dr Tiiu Kullisaar, fax +372 7 374312, email tiiukul@ut.ee
References
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Agerholm-Larsen, L, Raben, A, Haulrik, N, Hansen, AS, Manders, M & Astrup, A (2000) Effect of 8 weeks intake of probiotic milk products on risk factors for cardiovascular diseases. Eur J Clin Nutr 54, 288297.
Archibald, FS & Fridovich, I (1981) Manganese superoxide dismutase and oxygen tolerance in some lactic acid bacteria. J Bacteriol 146, 828936.
Broccali, G, Berti, M, Pistolesi, E & Gestaro, B (2000) Study of the effect of Lactobacillus GG supplementation in combination with and without arginine aspartate lipoproteins and liver peroxidation in cholesterol-fed rats. Int J Food Sci Nutr 51, 475482.
Demple, B, Hidalgo, E & Ding, H (1999) Transcriptional regulation via redox-sensitive iron-sulphur centers in an oxidative stress response. Biochem Soc Symp 64, 119128.
deRoos, NM & Katan, MB (2000) Effects of probiotic bacteria on diarrhea, lipid metabolism and carcinogenesis: a review of papers published between 1988 and 1998. Am J Clin Nutr 71, 405411.
de Vos, WM (1996) Metabolic engineering of sugar catabolism in lactic acid bacteria. Antonie Van Leeuwenhoek 70, 223224.
De Zwart, LL, Meerman, JHN, Commandeur, JN & Vermeulen, NPE (1999) Biomarkers of free radical damage applications in experimental animals and in humans. Free Radic Biol Med 26, 202226.
Epand, RM & Vogel, HJ (1999) Diversity of antimicrobial peptides and their mechanisms of action. Biochim Biophys Acta 1462, 1128.
Esterbauer, F, Striegl, G, Puhl, H & Rothenedr, M (1989) Continuous monitoring of in vitro oxidation of human low density lipoprotein. Free Radic Res Commun 6, 6775.
Falk, PG, Hooper, EH, Midtvedt, T & Gordon, JL (1998) Creating and maintaining the gastrointestinal ecosystem: what we know and need to know from gnotobiology. Microbiol Mol Biol Rev 62, 11571170.
Griffith, OW (1980) Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Anal Biochem 106, 207212.
Hancock, REW & Diamond, G (2000) The role of cationic antimicrobial peptides in innate host defence. Trends in Microbio 8, 402410.
Isolauri, E (2001) Probiotics in human disease. Am J Clin Nutr 73, 1142S1146S.
Isolauri, E, Arvola, T, Sütas, E, Moilanen, E & Salminen, S (2000) Probiotics in the management of atopic eczema. Clin Exp Allergy 30, 16041610.
Kailasapathy, K & Chin, J (2000) Survival and therapeutic potential of probiotic organisms with reference to Lactobacillus acidaphilus and Bifidobacterium spp. Immunol Cell Biol 78, 8088.
Kaizu, H, Sasaki, M, Nakajima, H & Suzuki, Y (1993) Effect of antioxidative lactic acid bacteria on rats fed a diet deficient in vitamin E. J Dairy Sci 46, 24932499.
Kullisaar, T, Zilmer, M & Mikelsaar, M (2002) Two antioxidative lactobacilli strains as promising probiotics. Int J Food Microbiol 72, 215224.
Lenzner, AA, Lenzner, ChP & Mikelsaar, M (1984) Die quantitative zusammensetzung der lactoflora des verdauungstrakts vor und nach kosmischen flügen unterschiedlicher dauer (The quantitative composition of the gut lactoflora before and after cosmic flights of different duration). Nahrung 28, 607613.
Lenzner, HP & Lenzner, AA (1982) Lysozyme activity and susceptibility of Lactobacilli to lysozyme of human microflora lactobacilli. In Mikrobielle Umwelt und anti-mikrobielle Massnahmen (Microbial Environment and Antimicrobial Activity), pp. 7381 [Knoke, M, editor]. Leipzig: M Johann Ambrosius Barth Verlag.
Lin, MY & Chang, FY (2000) Antioxidative effect of intestinal bacteria Bifidobacterium longum ATCC 15708 and Lactobacillus acidophilus ATCC 4356. Dig Dis Sci 45, 16171622.
Lin, MY & Yen, CL (1999) Antioxidative ability of lactic acid bacteria. J Agric Food Chem 47, 14601466.
Ljungh, A, Lan, J & Yanagisawa, N (2002) Isolation, selection and characteristics of Lactobacillus paracasei subsp. paracasei F19. Microb Ecol Health Dis Suppl. 3, 46.
Lowry, OH, Rosenbrough, NJ, Farr, AL & Randall, RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193, 265275.
McFarland, LV (2000) Beneficial microbes: health or hazard? Eur J Gastroenterol Hepatol 12, 10691071.
Mändar, R, Mändar, H & Mikelsaar, M (1992) Bioquant – a program for evaluation of faecal microbiocenosis In 1. Baltic Congress of Laboratory Medicine. Clin Chem Lookout 56.
Mikelsaar, M, Annuk, H, Shchepetova, J, Mändar, R, Sepp, E & Björksten, B (2002) Intestinal lactobacilli of Estonian and Swedish children. Microb Ecol Health Dis 14, 7580.
Mikelsaar, M, Lenzner, A & Goljanova, LA (1972) Methods for the estimation of the quantitative composition of faecal microflora. Lab Delo 1, 4145.
Mikelsaar, M, Mändar, R & Sepp, E (1998) Lactic acid microflora in the human microbial ecosystem and its development. In Lactic Acid Bacteria, Microbiology and Functional Aspects, pp. 279343 [Salminen, S and von Wright, A, editors]. New York: M. Dekker, Inc.
Morrow, JD, Frei, B, Longmire, AW et al. (1995) Increase in circulating products of lipid peroxidation (F2-isoprostanes) in smokers. New Eng J Med 332, 11981203.
Nakagawa, K, Ninomiya, M & Okubo, T (2000) In vitro and vivo antioxidant properties of gliclazide. J Diabetes Complications 14, 201206.
Nyyssönen, K, Porkkola, E, Salonen, R, Korpela, H & Salonen, JT (1994) Increase in oxidation resistance of atherogenic serum lipoproteins following antioxidant supplementation: a randomized double-blind placebo-controlled clinical trial. Eur J Clin Nutr 48, 633642.
O'Brien, RC, Luo, M, Balazs, & Mecuri, J (2000) In vitro and vivo antioxidant properties of gliclazide. J Diabetes Complications 14, 201206.
Oxman, T, Shapira, M, Diver, A, Klein, R, Avazov, N & Rabinowitz, B (2000) A new method of long-term preventive cardioprotaction using Lactobacillus. Am J Physiol 278, H1717H1724.
Patrono, C & Fitzgerald, GA (1997) Isoprostanes: potential markers of oxidant stress in atherothrombotic disease. Arterioscler Thromb Vasc Biol 17, 23092315.
Pähkla, R, Zilmer, M, Kullisaar, T & Rägo, L (1998) Comparison of the antioxidant activity of melatonin and pinolin in vitro. J Pineal Res 24, 96101.
Rice-Evans, C & Miller, NJ (1994) Total antioxidant status in plasma and body fluids. Methods Enzymol 234, 279293.
Roberts, JM & Cooper, DW (2001) Pathogenesis and genetics of pre-eclampsia. Lancet 357, 5355.
Sepp, E, Julge, K, Vasar, M, Naaber, P, Björgsten, B & Mikelsaar, M (1997) Intestinal microflora of Estonian and Swedish children. Acta Pediatr 86, 956961.
The R Project (2002) The R project for statistical computing. http://www.r-project.org.
Terahara, M, Nishide, S & Kaneko, T (2000) Preventive effect of Lactobacillus delbrueckii subsp bulgaricus on the oxidation of LDL. Biosci Biotechnol Biochem 64, 18681873.
Zhang, A, Vertommen, G, Van Gaal, L & De Leeun, I (1994) A rapid and simple method for measuring the susceptibility of low-density-lipoprotein and very-low-density-lipoprotein to copper catalyzed oxidation. Clin Chim Acta 227, 159173.
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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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