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Assay and digestion of 14C-labelled condensed tannins in the gastrointestinal tract of sheep

  • T. H. Terrill (a1), G. C. Waghorn (a2), D. J. Woolley (a3), W. C. Mcnabb (a1) and T. N. Barry (a1)
  • DOI:
  • Published online: 09 March 2007

Three experiments were conducted to determine the fate of condensed tannins (CT) during digestion in sheep. CT were measured as extractable, protein-bound and fibre-bound fractions using the butanol-HCI procedure. In Expt 1, purified CT were added to digesta from different parts of the digestive tract obtained from a pasture-fed sheep. Recoveries of CT after 0 and 4 h of anaerobic incubation at 39° averaged: rumen 78.9 and 57.5 %; abomasum 50.9 and 49.0 %; duodenum 64.4 and 46.0 % and ileum 43.4 and 38.8%. In Expt 2, [14C]CT was given per abomasum over a 6.5 h period at 15 min intervals to a sheep previously fed on Lotus pedunculatus (which contains CT). The sheep was killed at the end of the period and 92.4% of the label was recovered. Virtually all of the label was in the digesta, and none was detected in the blood, so that the CT-carbon appeared not to be absorbed from the small intestine. In Expt 3, rumen, abomasal and ileal digesta and faeces samples from sheep fed on Lotus pedunculatus were analysed for CT and CT flow along the digestive tract calculated from reference to indigestible markers. Values were low in all digesta samples, indicating disappearance of CT across the rumen and small intestine, and CT recovery in faeces was only about 15% of intake. However, the 14C results from Expt 2 suggested that little if any CT-carbon was absorbed and the low recoveries in Expt 1 are considered to be a consequence of either conformational changes to the CT molecule such that it is no longer detectable by colorimetric methods, an inability of the analytical method to release bound CT for the butanol–HCI assay, or interference from other digesta constituents. It is concluded that the butanol–HCI method of CT analysis is appropriate for quantifying CT in herbages but not in digesta or faeces, and that a substantial part of CT released during protein digestion in the small intestine may not be detectable by normal CT analytical methods.

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(). . , –.E. C. Bate-Smith 1973 Tannins of herbaceous leguminosae Phytochemistry 12 18091812

& (). . , –.R. B. Broadhurst W. T. Jones 1978 Analysis of condensed tannins using acidified vanillin Journal of the Science of Food and Agriculture 29 788794

, & (). . , –.N. T. Hinks S. C. Mills B. P. Setchell 1966 A simple method for the determination of specific activity of carbon dioxide in blood Analytical Biochemistry 17 551553

, & (). . , –.W. T. Jones R. B. Broadhurst J. W. Lyttleton 1976 The condensed tannins of pasture legume species Phytochemistryl 15 14071409

& (). . , –.W. T. Jones J. L. Mangan 1977 Complexes of the condensed tannins of the sainfoin (Onobrychis viciifolia Scop.) with Fraction 1 leaf protein and with submaxillary muco-protein, and their reversal by polyethylene glycol and pH Journal of the Science of Food and Agriculture 28 126136

, & (). . , –.R. Lai D. J. Woolley G. S. Lawes 1988 Patterns of assimilate transport from leaves to fruit within a kiwifruit (Acfinidia deliciosa) lateral Journal of Horticultural Science 63 725730

, , & (). . , –.W. C. McNabb G. C. Waghorn T. N. Barry I. D. Shelton 1993 The effect of condense tannins in Lotus pedunculatus on the digestion and metabolism of methionine, cystine and inorganic sulphur in sheep British Journal of Nutrition 70 647661

(). . , –.J. L. Mangan 1988 Nutritional effects of tannins in animal feeds Nutrition Research Reviews 1 209231

, & (). . , –.L. J. Porter L. N. Hrstich B. C. Chan 1986 The conversion of procyanidins and prodelphinidins to cyanidin and delphinidin Phytochemistry 25 223230

, & (). . , –.S. K. Sarkar R. E. Howarth B. P. Golpen 1976 Condensed tannins in herbaceous legumes Crop Science 16 543546

, , , , & (). . , –.T. H. Terrill G. B. Douglas A. G. Foote R. W. Purchas G. F. Wilson T. N. Barry 1992 aEffect of condensed tannins upon body growth, wool growth and rumen metabolism in sheep grazing sulla (Hedysarum coronarium) and perennial pasture Journal of Agricultural Science, Cambridge 119 265273

, , & (). . , –.T. H. Terrill A. M. Rowan G. B. Douglas T. N. Barry 1992 bDetermination of extractable and bound condensed tannin concentrations in forage plants, protein concentrate meals and cereal grains Journal of the Science of Food and Agriculture 58 321329

, , & (). . , –.T. H. Terrill W. R. Windham J. J. Evans C. S. Hoveland 1990 Condensed tannin concentrations in Sericea lespedeza, as influenced by preservation method Crop Science 30 219224

, , & (). . , –.T. H. Terrill W. R. Windham C. S. Hoveland H. E. Amos 1989 Forage preservation method influences on tannin concentration, intake and digestibility of Sericea Iespedeza by sheep Agronomy Journal 81 435439

, , & (). . , –.G.C. Waghorn I. D. Shelton W. C. McNabb S. N. McCutcheon 1994 bEffects of condensed tannin in Lotus pedunculatus on its nutritive value for sheep. 2. Nitrogenous aspects Journal of Agricultural Science, Cambridge 123 109119

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