Skip to main content
×
×
Home

Attenuation of diabetic complications by C-phycoerythrin in rats: antioxidant activity of C-phycoerythrin including copper-induced lipoprotein and serum oxidation

  • Badrish Soni (a1) (a2), Nishant P. Visavadiya (a1) and Datta Madamwar (a1)
Abstract

In the present study, the protective role of purified C-phycoerythrin (C-PE) against diabetic complications and Cu-mediated lipoprotein oxidation was evaluated. C-PE (25 and 50 mg/kg body weight per d) was administered to experimental streptozotocin–nicotinamide-induced type 2 diabetic male rats for 28 d. C-PE treatment successfully ameliorated diabetic complications by decreasing food intake, organ weights, serum concentrations of glucose, cholesterol, TAG, VLDL-cholesterol, creatinine, uric acid and thiobarbituric acid-reactive substances (TBARS), with increases in body weight, Hb, total protein, bilirubin and ferric-reducing ability of plasma values. Hepatic and renal tissues demonstrated significant decreases in TBARS, lipid hydroperoxide and conjugated diene contents, with increases in superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione, vitamin E and vitamin C levels. Furthermore, the 4-week ex vivo and in vitro administration of C-PE (0·5 and 1·0 mg/ml) indicated a decrease in Cu-mediated serum oxidation. The kinetics of the LDL oxidation profile showed significant prolongation of the lag phase with declines in oxidation rate, conjugated dienes, lipid hydroperoxide and TBARS. Results indicated the involvement of C-PE in the amelioration of diabetic complications by significant reductions in oxidative stress and oxidised LDL-triggered atherogenesis.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Attenuation of diabetic complications by C-phycoerythrin in rats: antioxidant activity of C-phycoerythrin including copper-induced lipoprotein and serum oxidation
      Available formats
      ×
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Attenuation of diabetic complications by C-phycoerythrin in rats: antioxidant activity of C-phycoerythrin including copper-induced lipoprotein and serum oxidation
      Available formats
      ×
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Attenuation of diabetic complications by C-phycoerythrin in rats: antioxidant activity of C-phycoerythrin including copper-induced lipoprotein and serum oxidation
      Available formats
      ×
Copyright
Corresponding author
*Corresponding author: Dr Datta Madamwar, fax +91 2692 231042, email datta_madamwar@yahoo.com
References
Hide All
1Dammeyer, T & Frankenberg-Dinkel, N (2006) Insights into phycoerythrobilin biosynthesis point toward metabolic channeling. J Biol Chem 281, 2708127089.
2Jensen, GS, Ginsberg, DI & Drapeau, C (2001) Blue-green algae as an immuno-enhancer and biomodulator. JANA 3, 2430.
3Belay, A (2002) The potential application of Spirulina (arthrospira) as a nutritional and therapeutic supplement in health management. JANA 5, 2648.
4Chiu, H-F, Yang, S-P, Kuo, Y-L, et al. (2006) Mechanisms involved in the antiplatelet effect of C-phycocyanin. Br J Nutr 95, 435440.
5Soni, B, Visavadiya, NP & Madamwar, D (2008) Ameliorative action of cyanobacterial phycoerythrin on CCl4-induced toxicity in rats. Toxicology 248, 5965.
6Evans, JL, Goldfine, ID, Maddux, BA, et al. (2002) Oxidative stress and stress-activated signaling pathways: a unifying hypothesis of type 2 diabetes. Endocrine Rev 23, 599622.
7Haffner, SM (2000) Obesity and the metabolic syndrome: the San Antonio Heart Study. Br J Nutr 83, Suppl. 1, S67S70.
8Brownlee, M (2001) Biochemistry and molecular cell biology of diabetic complications. Nature 414, 813820.
9Ravanan, R, Spiro, JR, Mathieson, PW, et al. (2007) Impact of diabetes on hemoglobin levels in renal disease. Diabetologia 50, 2631.
10Leeder, SR, Mitchell, P, Liew, G, et al. (2006) Low hemoglobin, chronic kidney disease and risk for coronary heart disease-related death: the Blue Mountains eye study. J Am Soc Nephrol 17, 279284.
11Kawamura, M, Heinecke, JW & Chait, A (1994) Pathophysiological concentrations of glucose promote oxidative modification of LDL by a superoxide-dependent pathway. J Clin Invest 94, 771778.
12Cameron, NE & Cotter, MA (1997) Metabolic and vascular factors in the pathogenesis of diabetic neuropathy. Diabetes 46, S31S37.
13Friedewald, WT, Levy, RT & Fredrickson, DS (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clin Chem 18, 499502.
14Benzie, IFF & Strain, JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of ‘antioxidant power’: the FRAP assay. Anal Biochem 239, 7076.
15Buege, JA & Aust, SD (1978) Microsomal lipid peroxidation. Methods Enzymol 52, 302310.
16Kim, YJ, Yokozawa, T & Chung, HY (2005) Effects of energy restriction and fish oil supplementation on renal guanidine levels and antioxidant defences in aged lupus-prone B/W mice. Br J Nutr 93, 835844.
17Jiang, ZY, Hunt, JV & Wolft, SD (1992) Ferrous ion oxidation in the presence of xylenol orange for detection of lipid hydroperoxide in low density lipoprotein. Anal Biochem 202, 384389.
18Kakkar, P, Das, B & Viswanathan, PN (1984) A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 21, 130132.
19Aebi, H (1984) Catalase in vitro. Methods Enzymol 105, 121126.
20Wendel, A (1981) Glutathione peroxidase. Methods Enzymol 77, 325333.
21Ellman, GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82, 7077.
22Martinek, RG (1964) Method for the determination of vitamin E (total tocopherols) in serum. Clin Chem 10, 10781086.
23Omaye, ST, Turnbull, JD & Sauberlich, HE (1979) Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids. Methods Enzymol 62, 311.
24Wieland, H & Seidel, D (1983) A simple specific method for precipitation of low density lipoproteins. J Lipid Res 24, 904909.
25Lowry, OH, Rosebrough, NJ, Farr, AL, et al. (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193, 265275.
26Palomäki, A, Malminiemi, K, Solakivi, T, et al. (1998) Ubiquinone supplementation during lovastatin treatment: effect on LDL oxidation ex vivo. J Lipid Res 39, 14301437.
27Esterbauer, H, Striegl, G, Puhl, H, et al. (1989) Continuous monitoring of in vitro oxidation of human low density lipoprotein. Free Radic Res Commun 6, 6775.
28Regnström, J, Ström, K, Moldeus, P, et al. (1993) Analysis of lipoprotein diene formation in human serum exposed to copper. Free Radic Res Commun 19, 267278.
29Masiello, P, Broca, C, Gross, R, et al. (1998) Experimental NIDDM: development of a new model in adult rats administered streptozotocin and nicotinamide. Diabetes 47, 224229.
30Szkudelski, T (2001) The mechanism of alloxan and streptozotocin action in β cells of the rat pancreas. Physiol Res 50, 536546.
31Nagaoka, S, Shimizu, K, Kaneko, S, et al. (2005) A novel protein C-phycocyanin plays a crucial role in the hypocholesterolemic action of Spirulina platensis concentrate in rats. J Nutr 135, 24252430.
32Fang, J & Alderman, MH (2000) Serum uric acid and cardiovascular mortality: The NHANES I Epidemiologic Follow-up Study, 1971–1992. J Am Med Assoc 283, 24042410.
33Jenkins, DJA, Kendall, CWC, Vidgen, E, et al. (2001) High-protein diets in hyperlipidemia: effect of wheat gluten on serum lipids, uric acid, and renal function. Am J Clin Nutr 74, 5763.
34Khan, M, Shobha, JC, Mohan, IK, et al. (2006) Spirulina attenuates cyclosporine-induced nephrotoxicity in rats. J Appl Toxicol 26, 444451.
35Bhat, VB & Madyastha, KM (2001) Scavenging of peroxynitrite by phycocyanin and phycocyanobilin from Spirulina platensis: protection against oxidative damage to DNA. Biochem Biophys Res Commun 285, 262266.
36Stocker, R, Yamamoto, Y, McDonagh, AF, et al. (1987) Bilirubin is an antioxidant of possible physiological importance. Science 235, 10431046.
37Stocker, R (2004) Antioxidant activities of bile pigments. Antioxid Redox Signal 6, 841849.
38Llesuy, SF & Tomaro, ML (1994) Heme oxygenase and oxidative stress. Evidence of involvement of bilirubin as physiological protector against oxidative damage. Biochim Biophys Acta 1223, 914.
39Gourley, GR (1997) Bilirubin metabolism and kernicterus. Adv Pediatr 44, 173229.
40Sedlak, TW & Snyder, SH (2004) Bilirubin benefits: cellular protection by a biliverdin reductase antioxidant cycle. Pediatrics 113, 17761782.
41Gieseg, SP & Esterbauer, H (1994) Low density lipoprotein is saturable by pro-oxidant copper. FEBS Lett 343, 188194.
42Ryter, SW, Alam, J & Choi, AMK (2006) Heme oxygenase-1/carbon monoxide: from basic science to therapeutic applications. Physiol Rev 86, 583650.
43Schuster, B, Prassl, R, Nigon, F, et al. (1995) Core lipid structure is a major determinant of the oxidative resistance of low density lipoprotein. Proc Natl Acad Sci U S A 92, 25092513.
44Wu, TW, Fung, KP, Wu, J, et al. (1996) Antioxidation of human low density lipoprotein by unconjugated and conjugated bilirubins. Biochem Pharmacol 51, 859862.
45Novotny, L & Vítek, N (2003) Inverse relationship between serum bilirubin and atherosclerosis in men: a meta-analysis of published studies. Exp Biol Med 228, 568571.
46Zhou, Z-P, Liu, L-N, Chen, X-L, et al. (2005) Factors that effect antioxidant activity of C-phycocyanins from Spirulina platensis. J Food Biochem 29, 313322.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Metrics

Full text views

Total number of HTML views: 5
Total number of PDF views: 67 *
Loading metrics...

Abstract views

Total abstract views: 134 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 25th April 2018. This data will be updated every 24 hours.