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Bitter melon (Momordica charantia L.) inhibits adipocyte hypertrophy and down regulates lipogenic gene expression in adipose tissue of diet-induced obese rats

  • Hui-Ling Huang (a1) (a2), Ya-Wen Hong (a1), You-Hong Wong (a3), Ying-Nien Chen (a4), Jong-Ho Chyuan (a5), Ching-Jang Huang (a6) (a7) and Pei-Min Chao (a1) (a3)...
Abstract

Bitter melon (Momordica charantia; BM) has been shown to ameliorate diet-induced obesity and insulin resistance. To examine the effect of BM supplementation on cell size and lipid metabolism in adipose tissues, three groups of rats were respectively fed a high-fat diet supplemented without (HF group) or with 5 % lyophilised BM powder (HFB group), or with 0·01 % thiazolidinedione (TZD) (HFT group). A group of rats fed a low-fat diet was also included as a normal control. Hyperinsulinaemia and glucose intolerance were observed in the HF group but not in HFT and HFB groups. Although the number of large adipocytes (>180 μm) of both the HFB and HFT groups was significantly lower than that of the HF group, the adipose tissue mass, TAG content and glycerol-3-phosphate dehydrogenase activity of the HFB group were significantly lower than those of the HFT group, implying that BM might reduce lipogenesis in adipose tissue. Experiment 2 was then conducted to examine the expression of lipogenic genes in adipose tissues of rats fed low-fat, HF or HFB diets. The HFB group showed significantly lower mRNA levels of fatty acid synthase, acetyl-CoA carboxylase-1, lipoprotein lipase and adipocyte fatty acid-binding protein than the HF group (P < 0·05). These results indicate BM can reduce insulin resistance as effective as the anti-diabetic drug TZD. Furthermore, BM can suppress the visceral fat accumulation and inhibit adipocyte hypertrophy, which may be associated with markedly down regulated expressions of lipogenic genes in the adipose.

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      Bitter melon (Momordica charantia L.) inhibits adipocyte hypertrophy and down regulates lipogenic gene expression in adipose tissue of diet-induced obese rats
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Corresponding author
*Corresponding author: Dr Pei-Min Chao, fax +886 4 2206 2891, email pmchao@mail.cmu.edu.tw
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1J Plutzky (2000) Emerging concepts in metabolic abnormalities associated with coronary artery disease. Curr Opin Cardiol 15, 416421.

2JK Grover & SP Yadav (2004) Pharmacological actions and potential uses of Momordica charantia: a review. J Ethnopharmacol 93, 123132.

3MB Krawinkel & GB Keding (2006) Bitter gourd (Momordica charantia): a dietary approach to hyperglycemia. Nutr Rev 64, 331337.

4CY Chao & CJ Huang (2003) Bitter gourd (Momordica charantia) extract activates peroxisome proliferator-activated receptors and upregulates the expression of the acyl CoA oxidase gene in H4IIEC3 hepatoma cells. J Biomed Sci 10, 782791.

5B Desvergne & W Wahli (1999) Peroxisome proliferator-activated receptors: nuclear control of metabolism. Endocr Rev 20, 649688.

6JP Berger , TE Akiyama & PT Meinke (2005) PPARs: therapeutic targets for metabolic disease. Trends Pharmacol Sci 26, 244251.

10P Arner (2005) Insulin resistance in type 2 diabetes – role of the adipokines. Curr Mol Med 5, 333339.

11KG Lambe & JD Tugwood (1996) A human peroxisome-proliferator-activated receptor-γ is activated by inducers of adipogenesis, including thiazolidinedione drugs. Eur J Biochem 239, 17.

17GY Yeh , DM Eisenberg , TJ Kaptchuk & RS Phillips (2003) Systematic review of herbs and dietary supplements for glycemic control in diabetes. Diabetes Care 26, 12771294.

18I Ahmed , E Adeghate , AK Sharma , DJ Pallot & J Singh (1998) Effects of Momordica charantia fruit juice on islet morphology in the pancreas of the streptozotocin-diabetic rat. Diabetes Res Clin Pract 40, 145151.

19T Miura , C Itoh , N Iwamoto , (2001) Hypoglycemic activity of the fruit of the Momordica charantia in type 2 diabetic mice. J Nutr Sci Vitaminol 47, 340344.

21BA Shibib , LA Khan & R Rahman (1993) Hypoglycaemic activity of Coccinia indica and Momordica charantia in diabetic rats: depression of the hepatic gluconeogenic enzymes glucose-6-phosphatase and fructose-1,6-bisphosphatase and elevation of both liver and red-cell shunt enzyme glucose-6-phosphate dehydrogenase. Biochem J 292, 267270.

22TB Ng , CM Wong , WW Li & HW Yeung (1986) Insulin-like molecules in Momordica charantia seeds. J Ethnopharmacol 15, 107117.

23E Cummings , HS Hundal , H Wackerhage , M Hope , M Belle , E Adeghate & J Singh (2004) Momordica charantia fruit juice stimulates glucose and amino acid uptakes in L6 myotubes. Mol Cell Biochem 261, 99104.

24P De Vos , AM Lefebvre , SG Miller , M Guerre-Millo , K Wong , R Saladin , LG Hamann , B Staels , MR Briggs & J Auwerx (1996) Thiazolidinediones repress ob gene expression in rodents via activation of peroxisome proliferator-activated receptor γ. J Clin Invest 98, 10041009.

26A Okuno , H Tamemoto , K Tobe , (1998) Troglitazone increases the number of small adipocytes without the change of white adipose tissue mass in obese Zucker rats. J Clin Invest 101, 13541361.

27I Bogacka , H Xie , GA Bray & SR Smith (2004) The effect of pioglitazone on peroxisome proliferator-activated receptor-γ target genes related to lipid storage in vivo. Diabetes Care 27, 16601667.

28T Deng , S Shan , PP Li , ZF Shen , XP Lu , Z Cheng & ZQ Ning (2006) Peroxisome proliferator-activated receptor-γ transcriptionally up-regulates hormone-sensitive lipase via the involvement of specificity protein-1. Endocrinology 147, 875884.

29RS Ahima & JS Flier (2000) Adipose tissue as an endocrine organ. Trends Endocrinol Metab 11, 327332.

30GS Hotamisligil , P Peraldi , A Budavari , R Ellis , MF White & BM Spiegelman (1996) IRS-1-mediated inhibition of insulin receptor tyrosine kinase activity in TNF-α- and obesity-induced insulin resistance. Science 271, 665668.

31ME Griffin , MJ Marcucci , GW Cline , K Bell , N Barucci , D Lee , LJ Goodyear , EW Kraegen , MF White & GI Shulman (1999) Free fatty acid-induced insulin resistance is associated with activation of protein kinase C theta and alterations in the insulin signaling cascade. Diabetes 48, 12701274.

32JM Olefsky (1976) The effects of spontaneous obesity on insulin binding, glucose transport, and glucose oxidation of isolated rat adipocytes. J Clin Invest 57, 842851.

33E Hu , JB Kim , P Sarraf & BM Spiegelman (1996) Inhibition of adipogenesis through MAP kinase-mediated phosphorylation of PPARγ. Science 274, 21002103.

34MS Brown , & JL Goldstein (1997) The SREBP pathway: regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor. Cell 89, 331340.

35VW Yang , RJ Christy , JS Cook , TJ Kelly & MD Lane (1989) Mechanism of regulation of the 422(aP2) gene by cAMP during preadipocyte differentiation. Proc Natl Acad Sci U S A 86, 36293633.

36Y Zhang , JJ Repa , K Gauthier & DJ Mangelsdorf (2001) Regulation of lipoprotein lipase by the oxysterol receptors, LXR LXRβ. J Biol Chem 276, 4301843024.

39JB Kim & BM Spiegelman (1996) ADD1/SREBP1 promotes adipocytes differentiation and gene expression linked to fatty acid metabolism. Genes Dev 10, 10961107.

40DG Palmer , GA Rutter & JM Tavare (2002) Insulin-stimulated fatty acid synthase gene expression does not require increased sterol response element binding protein 1 transcription in primary adipocytes. Biochem Biophys Res Commun 291, 439443.

41F Bertile & T Raclot (2004) mRNA levels of SREBP-1c do not coincide with the changes in adipose lipogenic gene expression. Biochem Biophys Res Commun 325, 827834.

42M Sekiya , N Yahagi , T Matsuzaka , (2007) Sterol regulatory element-binding protein (SREBP)-1-independent regulation of lipogenic gene expression in adipocytes. J Lipid Res (Epublication ahead of print version 24 April 2007).

43LK Juvet , SM Andresen , GU Schuster , (2003) On the role of liver X receptors in lipid accumulation in adipocytes. Mol Endocrinol 17, 172182.

44C Darimont , O Avanti , I Zbinden , P Leone-Vautravers , R Mansourian , V Giusti & K Mace (2006) Liver X receptor preferentially activates de novo lipogenesis in human preadipocytes. Biochimie 88, 309318.

45TM Stulnig , KR Steffensen , H Gao , M Reimers , K Dahlman-Wright , GU Schuster & J Gustafsson (2002) Novel roles of liver X receptors exposed by gene expression profiling in liver and adipose tissue. Mol Pharmacol 62, 12991305.

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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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