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Changes in gut microbiota due to supplemented fatty acids in diet-induced obese mice

  • Jorge R. Mujico (a1), Gyselle C. Baccan (a2), Alina Gheorghe (a1), Ligia E. Díaz (a1) and Ascensión Marcos (a1)
  • DOI:
  • Published online: 10 January 2013

Consumption of a high-fat diet (HFD), which is associated with chronic ‘low-grade’ systemic inflammation, alters the gut microbiota (GM). The aim of the present study was to investigate the ability of an oleic acid-derived compound (S1) and a combination of n-3 fatty acids (EPA and DHA, S2) to modulate both body weight and the GM in HFD-induced obese mice. A total of eighty mice were fed either a control diet or a HFD, non-supplemented or supplemented with S1 or S2. At week 19, faeces were collected in order to analyse the GM. Group-specific primers for accurate quantification of several major bacterial groups from faecal samples were assayed using quantitative PCR. The HFD induced an increase in body weight, which was reduced by supplementation with S1. Furthermore, S1 supplementation markedly increased total bacterial density and restored the proportions of bacteria that were increased (i.e. clostridial cluster XIVa and Enterobacteriales) or decreased (i.e. Bifidobacterium spp.) during HFD feeding. S2 supplementation significantly increased the quantities of Firmicutes (especially the Lactobacillus group). Correlation analysis revealed that body weight correlated positively with the phylum Firmicutes and clostridial cluster XIVa, and negatively with the phylum Bacteroidetes. In conclusion, the consumption of a HFD induced changes in the faecal microbiota, which were associated with the appearance of an obese phenotype. Supplementation of the HFD with S1 counteracted HFD-induced gut dysbiosis, together with an improvement in body weight. These data support a role for certain fatty acids as interesting nutrients related to obesity prevention.

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*Corresponding author: Dr J. R. Mujico, fax +34 915493627, email
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2EM Dewulf , PD Cani , AM Neyrinck , et al. (2011) Inulin-type fructans with prebiotic properties counteract GPR43 overexpression and PPARgamma-related adipogenesis in the white adipose tissue of high-fat diet-fed mice. J Nutr Biochem 22, 712722.

3GS Hotamisligil (2006) Inflammation and metabolic disorders. Nature 444, 860867.

4LV Hooper , MH Wong , A Thelin , et al. (2001) Molecular analysis of commensal host–microbial relationships in the intestine. Science 291, 881884.

5I Sekirov , SL Russell , LC Antunes , et al. (2010) Gut microbiota in health and disease. Physiol Rev 90, 859904.

6AL Kau , PP Ahern , NW Griffin , et al. (2011) Human nutrition, the gut microbiome and the immune system. Nature 474, 327336.

7A Guarino , A Wudy , F Basile , et al. (2012) Composition and roles of intestinal microbiota in children. J Matern Fetal Neonatal Med 25, Suppl. 1, 6366.

8F Shanahan (2009) Therapeutic implications of manipulating and mining the microbiota. J Physiol 587, 41754179.

9GD Wu , J Chen , C Hoffmann , et al. (2011) Linking long-term dietary patterns with gut microbial enterotypes. Science 334, 105108.

10BS Wostmann (1981) The germfree animal in nutritional studies. Ann Rev Nutr 1, 257279.

11I Nadal , A Santacruz , A Marcos , et al. (2009) Shifts in clostridia, bacteroides and immunoglobulin-coating fecal bacteria associated with weight loss in obese adolescents. Int J Obes (Lond) 33, 758767.

12A Santacruz , A Marcos , J Warnberg , et al. (2009) Interplay between weight loss and gut microbiota composition in overweight adolescents. Obesity (Silver Spring) 17, 19061915.

13CB de La Serre , CL Ellis , J Lee , et al. (2010) Propensity to high-fat diet-induced obesity in rats is associated with changes in the gut microbiota and gut inflammation. Am J Physiol Gastrointest Liver Physiol 299, G440G448.

14PJ Turnbaugh , RE Ley , MA Mahowald , et al. (2006) An obesity-associated gut microbiome with increased capacity for energy harvest. Nature 444, 10271031.

15PD Cani , J Amar , MA Iglesias , et al. (2007) Metabolic endotoxemia initiates obesity and insulin resistance. Diabetes 56, 17611772.

16PD Cani , AM Neyrinck , F Fava , et al. (2007) Selective increases of bifidobacteria in gut microflora improve high-fat-diet-induced diabetes in mice through a mechanism associated with endotoxaemia. Diabetologia 50, 23742383.

19C Razquin , JA Martinez , MA Martinez-Gonzalez , et al. (2009) A 3 years follow-up of a Mediterranean diet rich in virgin olive oil is associated with high plasma antioxidant capacity and reduced body weight gain. Eur J Clin Nutr 63, 13871393.

20O Vögler , A Lopez-Bellan , R Alemany , et al. (2008) Structure–effect relation of C18 long-chain fatty acids in the reduction of body weight in rats. Int J Obes (Lond) 32, 464473.

21JD Buckley & PR Howe (2010) Long-chain omega-3 polyunsaturated fatty acids may be beneficial for reducing obesity – a review. Nutrients 2, 12121230.

22PB Eckburg , EM Bik , CN Bernstein , et al. (2005) Diversity of the human intestinal microbial flora. Science 308, 16351638.

24F Backhed , RE Ley , JL Sonnenburg , et al. (2005) Host–bacterial mutualism in the human intestine. Science 307, 19151920.

25T Rinttila , A Kassinen , E Malinen , et al. (2004) Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR. J Appl Microbiol 97, 11661177.

26F Backhed & PA Crawford (2010) Coordinated regulation of the metabolome and lipidome at the host–microbial interface. Biochim Biophys Acta 1801, 240245.

27PD Cani & NM Delzenne (2009) The role of the gut microbiota in energy metabolism and metabolic disease. Curr Pharm Des 15, 15461558.

28N Larsen , FK Vogensen , FW van den Berg , et al. (2010) Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults. PloS One 5, e9085.

29F Armougom , M Henry , B Vialettes , et al. (2009) Monitoring bacterial community of human gut microbiota reveals an increase in Lactobacillus in obese patients and methanogens in anorexic patients. PloS One 4, e7125.

30PD Cani & NM Delzenne (2009) Interplay between obesity and associated metabolic disorders: new insights into the gut microbiota. Curr Opin Pharmacol 9, 737743.

31MA Hildebrandt , C Hoffmann , SA Sherrill-Mix , et al. (2009) High-fat diet determines the composition of the murine gut microbiome independently of obesity. Gastroenterology 137, 17161724.e1-2.

32C Lupp , ML Robertson , ME Wickham , et al. (2007) Host-mediated inflammation disrupts the intestinal microbiota and promotes the overgrowth of Enterobacteriaceae. Cell Host Microbe 2, 204.

34RE Ley , F Backhed , P Turnbaugh , et al. (2005) Obesity alters gut microbial ecology. Proc Natl Acad Sci U S A 102, 1107011075.

35RE Ley , PJ Turnbaugh , S Klein , et al. (2006) Microbial ecology: human gut microbes associated with obesity. Nature 444, 10221023.

37A Schwiertz , D Taras , K Schafer , et al. (2010) Microbiota and SCFA in lean and overweight healthy subjects. Obesity (Silver Spring) 18, 190195.

38F Backhed , H Ding , T Wang , et al. (2004) The gut microbiota as an environmental factor that regulates fat storage. Proc Natl Acad Sci U S A 101, 1571815723.

39G Musso , R Gambino & M Cassader (2010) Gut microbiota as a regulator of energy homeostasis and ectopic fat deposition: mechanisms and implications for metabolic disorders. Curr Opin Lipidol 21, 7683.

41E Ringo , HR Bendiksen , SJ Gausen , et al. (1998) The effect of dietary fatty acids on lactic acid bacteria associated with the epithelial mucosa and from faecalia of Arctic charr, Salvelinus alpinus (L.). J Appl Microbiol 85, 855864.

42PE Kankaanpaa , SJ Salminen , E Isolauri , et al. (2001) The influence of polyunsaturated fatty acids on probiotic growth and adhesion. FEMS Microbiol Lett 194, 149153.

44BD Pachikian , AM Neyrinck , L Portois , et al. (2011) Involvement of gut microbial fermentation in the metabolic alterations occurring in n-3 polyunsaturated fatty acids-depleted mice. Nutr Metab 8, 44.

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British Journal of Nutrition
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