Skip to main content Accessibility help
×
×
Home

Cholecalciferol supplementation alters gut function and improves digestibility in an underground inhabitant, the naked mole rat (Heterocephulus gluber), when fed on a carrot diet

  • Shlomo Yahav (a1) and Rochelle Buffenstein (a2)

Abstract

Naked mole rats (Heterocephalus glaber) lead a strictly subterranean existence and appear to be naturally deficient in cholecalciferol (D3). Oral supplementation with D3 (Ds) led to a 1.8-fold increase in food intake and the associated enlargement (1.4-fold) of the caecum. The effect of Ds, and the concomitant increase in food intake, on caecal fermentation efficiency when animals were fed on a carrotbased diet was determined by measuring the rate of both gas production and short-chain fatty acid (SCFA) production. Microbial-controlled fermentation processes in the caecum were enhanced with Ds when compared with animals not receiving a D3 supplement (Dn). Both the rates of gas production (Dn 10.76 (SE 0.77), Ds 15.20 (SE 1.77) ml/g dry matter (DM) per h) and SCFA production (Dn 463.0 (SE 33.7), Ds 684.3 (SE 74.8) μmol/g DM per h) increased more than 1.4-fold per g DM caecal substrate. These factors contributed to the higher digestibility of the food in Ds animals. The larger quantity of energy available to D3-replete naked mole rats was not used in anabolic processes, for these animals maintained mass. These findings suggest that metabolic rate in D3-replete animals was elevated. Thus, despite improved gut function, D3-replete animals may be disadvantaged by their higher energy and food requirements in their natural milieu.

Copyright

References

Hide All
American Institute of Nutrition (1977). Report of the A.I.N. ad hoe committee on standards for nutritional studies. Journal of Nutrition 107, 1340.
Buffenstein, R., Jarvis, J. U. M., Opperman, L. A., Cavaleros, M., Hough, S. & Ross, P. (1988). Vitamin D metabolism and expression in chthonic naked mole rats. Journal of Bone and Mineral Research 3, S119 Abstr.
Buffenstein, R., Skinner, D. C., Yahav, S., Moodley, G. P., Cavaleros, M., Zachen, D., Ross, F. P. & Pettifor, J. M. (1991). Effect of oral cholecalciferol supplementation at physiological and supraphysiological doses in naturally D3-deficient subterranean damdra mole rats, Cryptomys damarensis. Journal of Endocrinology 131, 197202.
Buffenstein, R. & Yahav, S. (1991 a). The effect of diet on microbial population and function in the caecum of the subterranean naked mole rat (Heterocephalus glaber). British Journal of Nutrition 65, 249258.
Buffenstein, R. & Yahav, S. (1991 b). Cholecalciferol has no effect on calcium and inorganic phosphorus balance in a naturally cholecalciferol-deplete subterranean mammal, the naked mole rat (Heterocephalus glaber). Journal of Endocrinology 129, 2126.
Buffenstein, R. & Yahav, S. (1991 c). Is the naked mole-rat Heterocephalus glaber an endothermic yet poikilothermic mammal? Journal of Thermal Biology 16, 227232.
Carol, E. J. & Hungate, R. E. (1954). The magnitude of microbial fermentation in bovine rumen. Applied Microbiology 2, 205214.
Chertow, B. S., Sivitz, W. I., Baranetsky, N. G., Clark, S. A., Waite, A. & Deluca, H. F. (1983). Cellular mechanisms of insulin release: The effects of vitamin D deficiency and repletion on rat insulin secretion. Endocrinology 113, 15111518.
Clark, S. A., D'ercole, A. J. & Toverud, S. U. (1986). Somatomedin-C/;sol;Insulin like growth factor I and vitamin D-induced growth. Endocrinology 119, 16601665.
Clarke, S. A., Stumpf, W. E. & Sar, M. (1981). Effect of 1,25-dihydroxyvitamin D3 on insulin secretion. Diabetes 30, 382386.
Davies, J. (1988). Laboratory Methods. Irene, Transvaal: Animal Nutrition Subdirectorate Animal and Dairy Science Research Institute.
EI-Din, M. Z. & EI-Shazly, K. (1969). Evaluation of a method of measuring fermentation rates and net growth of rumen micro-organisms. Applied Microbiology 17, 801804.
Gardener, R. M., Reinhardt, T. A. & Horst, R. L. (1988). The biological assessment of vitamin D3 metabolites produced by rumen bacteria. Journal of Steroid Biochemistry 29, 185189.
Jarvis, J. U. M. (1991). Methods for capturing, transporting and maintaining naked mole rats in captivity. In Biology of the Naked Mole Rat, pp. 467483 [Sherman, P. W., Jarvis, P. W. and Alexander, R. D., editors]. Princeton: Princeton University Press.
Jarvis, J. U. M. & Bennett, N. C. (1991). Ecology and behaviour of the family Bathyergidae. In Biology of the Naked Mole Rat, pp. 6669 [Sherman, P. W., Jarvis, J. U. M. and Alexander, R. D., editors]. Princeton: Princeton University Press.
Kadowaki, S. & Norman, A. W. (1984). Dietary vitamin D is essential for normal insulin secretion from the perfused rat pancreas. Journal of Clinical Investigation 73, 759–166.
McBee, R. H. (1970). Metabolic contributions of the caecal flora. American Journal of Clinical Nutririon 23, 15141518.
Norman, A. W., Frankel, B. J., Heldt, A. M. & Grodsky, M. (1980). Vitamin D deficiency inhibits pancreatic secretion of insulin. Science 209, 823825.
Nyomba, B. L., Bouillon, R. & De Moor, P. (1984). Influence of vitamin D status on insulin secretion and glucose tolerance in the rabbit. Endocrinology 115, 191197.
Rechkemmer, G., Ronnau, K. & Engelhardt, W. W. (1988). Fermentation of polysaccharides and absorption of short chain fatty acids in the mammalian hindgut. Comparative Biochemistry and Physiology 90A, 563568.
Sibly, R. M. (1981). Strategies in digestion and defecation. In Physiological Ecology, and Evolutionary Approach to Resource Use, pp. 109139 [Townsend, C. R. and Calow, P., editors]. Sunderland, Massachusetts: Sinauer Association.
Skinner, D. C., Moodley, G. & Buffenstein, R. (1991). Is Vitamin D3 essential for mineral metabolism in the Damara mole rat (Cryptomys damarensis)? General and Cotnparative Endocrinology 81, 501505.
Sommerfeldt, J. L., Horst, R. L., Napoli, J. L., Beitz, D. C. & Littledike, E. T. (1980). Evidence for in-vitro production of vitamin D2 and vitamin D3 metabolites by rumen microbes. Journal of Dairy Science 63, Suppl. 1 , 8889.
Stumpf, W. E., Sar, M. & Clark, S. A. (1982). Brain target sites for 1,25-dihydroxyvitamin D3. Science 215, 14041405.
Van Soest, P. J. (editor) (1982). Gastrointestinal fermentation. In Nutritional Ecology of the Rummants, pp. 152229. Corvallis, Oregon: O. and B. Books.
Vleck, D. (1981). Burrow structure and foraging costs in the fossorial rodent, Thomomys bottae. Oecologia 49, 391396.
Yahav, S. & Buffenstein, R. (1991). The effect of temperature on caecal fermentation processes in a poikilothermic mammal, Heterocephalus glaber. Journal of Thermal Biology 16, 345349.
Zar, J. H. (1974). Biostatistiral Analysis. Englewood Cliffs, New Jersey: Prentice Hall.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed