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Comparison of the effects of a high- and normal-casein breakfast on satiety, ‘satiety’ hormones, plasma amino acids and subsequent energy intake

  • Margriet A. B. Veldhorst (a1) (a2), Arie G. Nieuwenhuizen (a1) (a2), Ananda Hochstenbach-Waelen (a1) (a2), Klaas R. Westerterp (a1) (a2), Marielle P. K. J. Engelen (a3), Robert-Jan M. Brummer (a2), Nicolaas E. P. Deutz (a2) (a3) and Margriet S. Westerterp-Plantenga (a1) (a2)...

The present study compared the effects of a high- and normal-casein-protein breakfast on satiety, ‘satiety’ hormones, plasma amino acid responses and subsequent energy intake. Twenty-five healthy subjects (BMI 23·9 (sem 0·3) kg/m2; age 22 (sem 1) years) received a subject-specific standardised breakfast (20 % of daily energy requirements): a custard with casein as the single protein source with either 10, 55 and 35 (normal-casein breakfast) or 25, 55 and 20 (high-casein breakfast) % of energy (En%) from protein, carbohydrate and fat respectively in a randomised, single-blind design. Appetite profile (visual analogue scale; VAS), plasma glucose, insulin, glucagon-like peptide 1, ghrelin and amino acid concentrations were determined for 4 h; here the sensitive moment in time for lunch was determined. Subjects came for a second set of experiments and received the same custards for breakfast, and an ad libitum lunch was offered at 180 min after breakfast; energy intake was assessed. There were increased scores of fullness and satiety after the 25 En% casein-custard compared with the 10 En% casein-custard, particularly at 180 min (26 (sem 4) v. 11 (sem 5) mm VAS; P < 0·01) and 240 min (13 (sem 5) v. − 1 (sem 5) mm VAS; P < 0·01). This coincided with prolonged elevated plasma amino acid concentrations; total amino acids and branched-chain amino acids were higher after the 25 En% casein-custard compared with the 10 En% casein-custard at 180 and 240 min (P < 0·001). There was no difference in energy intake (3080 (sem 229) v. 3133 (sem 226) kJ for 25 En% and 10 En% respectively; NS) from the ad libitum lunch. In conclusion, a breakfast with 25 % of energy from casein is rated as being more satiating than a breakfast with 10 % of energy from casein at 3 and 4 h after breakfast, coinciding with prolonged elevated concentrations of plasma amino acids, but does not reduce subsequent energy intake.

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      Comparison of the effects of a high- and normal-casein breakfast on satiety, ‘satiety’ hormones, plasma amino acids and subsequent energy intake
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      Comparison of the effects of a high- and normal-casein breakfast on satiety, ‘satiety’ hormones, plasma amino acids and subsequent energy intake
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*Corresponding author: Dr Margriet Veldhorst, fax +31 43 3670976, email
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1Seidell, JC (1995) Obesity in Europe. Obes Res 3, Suppl. 2, 89s93s.
2Pi-Sunyer, FX (1993) Medical hazards of obesity. Ann Intern Med 119, 655660.
3Westerterp-Plantenga, MS, Luscombe-Marsh, N, Lejeune, MPGM, Diepvens, K, Nieuwenhuizen, A, Engelen, MPKJ, Deutz, NEP, Azzout-Marniche, D, Tome, D & Westerterp, KR (2006) Dietary protein, metabolism, and body-weight regulation: dose–response effects. Int J Obes (Lond) 30, Suppl. 3, S16S23.
4Skov, AR, Toubro, S, Ronn, B, Holm, L & Astrup, A (1999) Randomized trial on protein vs carbohydrate in ad libitum fat reduced diet for the treatment of obesity. Int J Obes Relat Metab Disord 23, 528536.
5Lejeune, MPGM, Kovacs, EM & Westerterp-Plantenga, MS (2005) Additional protein intake limits weight regain after weight loss in humans. Br J Nutr 93, 281289.
6Weigle, DS, Breen, PA, Matthys, CC, Callahan, HS, Meeuws, KE, Burden, VR & Purnell, JQ (2005) A high-protein diet induces sustained reductions in appetite, ad libitum caloric intake, and body weight despite compensatory changes in diurnal plasma leptin and ghrelin concentrations. Am J Clin Nutr 82, 4148.
7Clifton, PM, Keogh, JB & Noakes, M (2008) Long-term effects of a high-protein weight-loss diet. Am J Clin Nutr 87, 2329.
8Bertenshaw, EJ, Lluch, A & Yeomans, MR (2007) Satiating effects of protein but not carbohydrate consumed in a between-meal beverage context. Physiol Behav 83, 211220.
9Crovetti, R, Porrini, M, Santangelo, A & Testolin, G (1998) The influence of thermic effect of food on satiety. Eur J Clin Nutr 52, 482488.
10Halton, TL & Hu, FB (2004) The effects of high protein diets on thermogenesis, satiety and weight loss: a critical review. J Am Coll Nutr 23, 373385.
11Hall, WL, Millward, DJ, Long, SJ & Morgan, LM (2003) Casein and whey exert different effects on plasma amino acid profiles, gastrointestinal hormone secretion and appetite. Br J Nutr 89, 239248.
12Bowen, J, Noakes, M & Clifton, PM (2006) Appetite regulatory hormone responses to various dietary proteins differ by body mass index status despite similar reductions in ad libitum energy intake. J Clin Endocrinol Metab 91, 29132919.
13Bowen, J, Noakes, M, Trenerry, C & Clifton, PM (2006) Energy intake, ghrelin, and cholecystokinin after different carbohydrate and protein preloads in overweight men. J Clin Endocrinol Metab 91, 14771483.
14Lang, V, Bellisle, F, Oppert, JM, Craplet, C, Bornet, FR, Slama, G & Guy-Grand, B (1998) Satiating effect of proteins in healthy subjects: a comparison of egg albumin, casein, gelatin, soy protein, pea protein, and wheat gluten. Am J Clin Nutr 67, 11971204.
15Lang, V, Bellisle, F, Alamowitch, C, Craplet, C, Bornet, FR, Slama, G & Guy-Grand, B (1999) Varying the protein source in mixed meal modifies glucose, insulin and glucagon kinetics in healthy men, has weak effects on subjective satiety and fails to affect food intake. Eur J Clin Nutr 53, 959965.
16Uhe, AM, Collier, GR & O'Dea, K (1992) A comparison of the effects of beef, chicken and fish protein on satiety and amino acid profiles in lean male subjects. J Nutr 122, 467472.
17Miller, MJ, Witherly, SA & Clark, DA (1990) Casein: a milk protein with diverse biologic consequences. Proc Soc Exp Biol Med 195, 143159.
18Boirie, Y, Dangin, M, Gachon, P, Vasson, MP, Maubois, JL & Beaufrère, B (1997) Slow and fast dietary proteins differently modulate postprandial protein accretion. Proc Natl Acad Sci U S A 94, 1493014935.
19Dangin, M, Boirie, Y, Garcia-Rodenas, C, Gachon, P, Fauquant, J, Callier, P, Ballèvre, O & Beaufrère, B (2001) The digestion rate of protein is an independent regulating factor of postprandial protein retention. Am J Physiol Endocrinol Metab 280, E340E348.
20Mellinkoff, SM, Frankland, M, Boyle, D & Greipel, M (1956) Relationship between serum amino acid concentration and fluctuations in appetite. J Appl Physiol 8, 535538.
21Spaaij, CJ & Pijls, LT (2004) New dietary reference intakes in The Netherlands for energy, proteins, fats and digestible carbohydrates. Eur J Clin Nutr 58, 191194.
22Munro, HN (1976) Second Boyd Orr Memorial Lecture. Regulation of body protein metabolism in relation to diet. Proc Nutr Soc 35, 297308.
23Stunkard, AJ & Messick, S (1985) The three-factor eating questionnaire to measure dietary restraint, disinhibition and hunger. J Psychosom Res 29, 7183.
24Westerterp-Plantenga, MS, Westerterp, KR, Rubbens, M, Verwegen, CR, Richelet, JP & Gardette, B (1999) Appetite at “high altitude” [Operation Everest III (Comex-'97)]: a simulated ascent of Mount Everest. J Appl Physiol 87, 391399.
25Harris, JA & Benedict, FG (1919) A Biometric Study of Basal Metabolism in Man. Washington, DC: Carnegie Institute of Washington.
26Adam, TC & Westerterp-Plantenga, MS (2005) Nutrient-stimulated GLP-1 release in normal-weight men and women. Horm Metab Res 37, 111117.
27Stubbs, RJ, Hughes, DA, Johnstone, AM, Rowley, E, Reid, C, Elia, M, Stratton, R, Delargy, H, King, N & Blundell, JE (2000) The use of visual analogue scales to assess motivation to eat in human subjects: a review of their reliability and validity with an evaluation of new hand-held computerized systems for temporal tracking of appetite ratings. Br J Nutr 84, 405415.
28Flint, A, Raben, A, Blundell, JE & Astrup, A (2000) Reproducibility, power and validity of visual analogue scales in assessment of appetite sensations in single test meal studies. Int J Obes Relat Metab Disord 24, 3848.
29van Eijk, HM, Rooyakkers, DR & Deutz, NE (1993) Rapid routine determination of amino acids in plasma by high-performance liquid chromatography with a 2–3 microns Spherisorb ODS II column. J Chromatogr 620, 143148.
30Senn, S (2006) Cross-over trials in Statistics in Medicine: The first ‘25’ years. Stat Med 25, 34303442.
31Kirimura, J, Shimizi, A, Kimizuka, A, Ninomiya, T & Katsuya, N (1969) The contribution of peptides and amino acids to the taste of foodstuffs. J Agric Food Chem 17, 689695.
32Mahé, S, Roos, N, Benamouzig, R, Davin, L, Luengo, C, Gagnon, L, Gaussergès, N, Rautureau, J & Tomé, D (1996) Gastrojejunal kinetics and the digestion of [15N]β-lactoglobulin and casein in humans: the influence of the nature and quantity of the protein. Am J Clin Nutr 63, 546552.
33Foster-Schubert, KE, Overduin, J, Prudom, CE, Liu, J, Callahan, HS, Gaylinn, BD, Thorner, MO & Cummings, DE (2008) Acyl and total ghrelin are suppressed strongly by ingested proteins, weakly by lipids, and biphasically by carbohydrates. J Clin Endocrinol Metab 93, 19711979.
34Erdmann, J, Lippl, F & Schusdziarra, V (2003) Differential effect of protein and fat on plasma ghrelin levels in man. Regul Pept 116, 101107.
35Cummings, DE (2006) Ghrelin and the short- and long-term regulation of appetite and body weight. Physiol Behav 89, 7184.
36Lejeune, MP, Westerterp, KR, Adam, TC, Luscombe-Marsh, ND & Westerterp-Plantenga, MS (2006) Ghrelin and glucagon-like peptide 1 concentrations, 24-h satiety, and energy and substrate metabolism during a high-protein diet and measured in a respiration chamber. Am J Clin Nutr 83, 8994.
37Anderson, GH, Tecimer, SN, Shah, D & Zafar, TA (2004) Protein source, quantity, and time of consumption determine the effect of proteins on short-term food intake in young men. J Nutr 134, 30113015.
38Borzoei, S, Neovius, M, Barkeling, B, Teixeira-Pinto, A & Rossner, S (2006) A comparison of effects of fish and beef protein on satiety in normal weight men. Eur J Clin Nutr 60, 897902.
39van Milgen, J (2002) Modeling biochemical aspects of energy metabolism in mammals. J Nutr 132, 31953202.
40Pannemans, DL, Wagenmakers, AJ, Westerterp, KR, Schaafsma, G & Halliday, D (1998) Effect of protein source and quantity on protein metabolism in elderly women. Am J Clin Nutr 68, 12281235.
41Robinson, SM, Jaccard, C, Persaud, C, Jackson, AA, Jequier, E & Schutz, Y (1990) Protein turnover and thermogenesis in response to high-protein and high-carbohydrate feeding in men. Am J Clin Nutr 52, 7280.
42Westerterp-Plantenga, MS, Rolland, V, Wilson, SA & Westerterp, KR (1999) Satiety related to 24 h diet-induced thermogenesis during high protein/carbohydrate vs high fat diets measured in a respiration chamber. Eur J Clin Nutr 53, 495502.
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