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Curcumin induces paraoxonase 1 in cultured hepatocytes in vitro but not in mouse liver in vivo

  • Charlotte Schrader (a1), Christina Schiborr (a2), Jan Frank (a2) and Gerald Rimbach (a1)

Abstract

Paraoxonase 1 (PON1) is an enzyme that is mainly synthesised in the liver and protects LDL from oxidation, thereby exhibiting antiatherogenic properties. Using a luciferase reporter gene assay, we tested curcumin for its ability to induce PON1 in Huh7 hepatocytes in culture. Curcumin ( ≥ 10 μmol/l) dose-dependently induced PON1 transactivation in Huh7 cells. However, dietary supplementation of female B6C3F1 mice with curcumin (500 mg/kg diet) for 2 weeks did not increase the hepatic PON1 mRNA and protein levels. No curcumin was detectable in the plasma of the 12 h fasted mice. In conclusion, curcumin may be a potent PON1 inducer in cultured cells in vitro, but not in the liver of curcumin-fed mice because of its low concentrations in vivo.

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Copyright

Corresponding author

*Corresponding author: Professor G. Rimbach, fax +49 431 880 26 28, email rimbach@foodsci.uni-kiel.de

References

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Curcumin induces paraoxonase 1 in cultured hepatocytes in vitro but not in mouse liver in vivo

  • Charlotte Schrader (a1), Christina Schiborr (a2), Jan Frank (a2) and Gerald Rimbach (a1)

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