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Cyanidin-3-O-β-glucopyranoside, a natural free-radical scavenger against aflatoxin B1- and ochratoxin A-induced cell damage in a human hepatoma cell line (Hep G2) and a human colonic adenocarcinoma cell line (CaCo-2)

  • M. C. Guerra (a1), F. Galvano (a2), L. Bonsi (a3), E. Speroni (a1), S. Costa (a1), C. Renzulli (a1) and R. Cervellati (a4)
  • DOI:
  • Published online: 01 March 2007

Recent findings have suggested that oxidative damage might contribute to the cytotoxicity and carcinogenicity of aflatoxin B1 (AFB1). Induction of oxidative stress also plays an important role in the toxicity of another mycotoxin, ochratoxin A (OTA). In the present study, the protective effect of cyanidin-3-O-β-glucopyranoside (C-3-G; an anthocyanin contained in oranges, blackberries, strawberries and cranberries) against AFB1- and OTA-induced cytotoxicity was investigated in a human hepatoma-derived cell line (Hep G2) and a human colonic adenocarcinoma cell line (CaCo-2). The ability of C-3-G to reduce the production of reactive oxygen species (ROS), the inhibition of protein and DNA synthesis and the apoptosis caused by the two mycotoxins was also investigated in both cell lines. Our experiments proved the significant cytoprotective effect of C-3-G in vitro against OTA- and AFB1-induced cell damage. In particular, 24 h of pretreatment with 50 μm-C-3-G inhibited the cytotoxicity of 10 μm-AFB1 (by 35 %) and of 10 μm-OTA (by 25 %) in Hep G2 cells (P<0·001) and of 10 μm-AFB1 (by 10 %, P<0·01) and of 10 μm-OTA (by 14 %, P<0·05) in CaCo-2 cells. Moreover, 50 μm-C-3-G attenuated ROS production induced by the two toxins in both cell lines (P<0·05). Inhibition of DNA and protein synthesis induced by the mycotoxins was counteracted by pretreatment with the antioxidant at 50 μm. Similarly, apoptotic cell death was prevented as demonstrated by a reduction of DNA fragmentation and inhibition of caspase-3 activation. The in vitro free-radical scavenging capacity of the anthocyanin was tested with the Briggs–Rauscher oscillating reaction. This system works at pH approximately 2. The results showed good scavenging power, in accordance with the observed inhibition of ROS production.

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      Cyanidin-3-O-β-glucopyranoside, a natural free-radical scavenger against aflatoxin B1- and ochratoxin A-induced cell damage in a human hepatoma cell line (Hep G2) and a human colonic adenocarcinoma cell line (CaCo-2)
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      Cyanidin-3-O-β-glucopyranoside, a natural free-radical scavenger against aflatoxin B1- and ochratoxin A-induced cell damage in a human hepatoma cell line (Hep G2) and a human colonic adenocarcinoma cell line (CaCo-2)
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      Cyanidin-3-O-β-glucopyranoside, a natural free-radical scavenger against aflatoxin B1- and ochratoxin A-induced cell damage in a human hepatoma cell line (Hep G2) and a human colonic adenocarcinoma cell line (CaCo-2)
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Corresponding author
*Corresponding author: Professor Maria Clelia Guerra, fax +39 51 248862, email
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S Abel & WC Gelderblom (1998) Oxidative damage and fumonisin B1-induced toxicity in primary rat hepatocytes and rat liver in vivo. Toxicology 131, 121131.

P Amstad , A Levy , H Emeir & P Cerulti (1984) Evidence for membrane-medicated chromosomal damage by afflatoxin B1 in human lymphocytes. Carcinogenesis 5, 719723.

F Atroshi , J Parantainen , R Kangasniemi & S Sankari (1987) Sialic acid, glutathione metabolism, and electrical conductivity in bovine mastitis udder tissue. J Anim Physiol Anim Nutr 58, 200207.

I Baudrimont , AM Betbeder , A Gharbi , A Pfohl-Leszkowicz , G Dirheimer & EE Creppy (1994) Effect of superoxide dismutase and catalase on the nephrotoxicity induced by subchronical administration of ochratoxin A in rats. Toxicology 89, 101111.

A Belmadani , PS Steyn , G Tramu , AM Betbeder , I Baudrimont & EE Creppy (1999) Selective toxicity of ochratoxin A in primary cultures from different brain regions. Arch Toxicol 73, 108114.

MM Bradford (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248254.

T Briggs & W Rauscher (1973) An oscillating iodine clock. J Chem Educ 50, 496.

C Cavin , D Holzhäser , A Constable , AC Huggett & B Schilter (1998) The coffee-specific diterpenes cafestol and kahweol protect against aflatoxin B 1 -induced genotoxicity through a dual mechanism. Carcinogenesis 19, 13691375.

R Cervellati , K Höner , SD Furrow , C Neddens & S Costa (2001) The Briggs–Rauscher reaction as a test to measure the activity of antioxidants. Helv Chim Acta 84, 35333547.

R Cervellati , K Höner , SD Furrow , F Mazzanti & S Costa (2004a) An experimental mechanistic investigation of the complexities arising during inhibition of the Briggs–Rauscher reaction by antioxidants. Helv Chim Acta 87, 133155.

R Cervellati , G Innocenti , S Dall'Acqua , S Costa & E Sartini (2004) Polyphenols from Polygala spp. and their antioxidant activity. Chem Biodivers 1, 415425.

C Cochereau , D Sanchez & EE Creppy (1997) Tyrosine prevents capsaicin-induced protein synthesis inhibition in cultured cells. Toxicology 117, 133139.

C Fimognari , F Berti , M Nüsse , G Cantelli-Forti & P Hrelia (2004) Induction of apoptosis in two human leukemia cell lines as well as differentiation in human promyelocytic cells by cyanidin-3- O -β-glucopyranoside. Biochem Pharmacol 67, 20472056.

F Galvano , L La Fauci , G Lazzarino , V Fogliano , A Ritieni , S Ciappellano , NC Battistini , B Tavazzi & G Galvano (2004) Cyanidins: metabolism and biological properties. J Nutr Biochem 15, 211.

JC Gautier , D Holzhaeuser , J Markovic , E Gremaud , B Schilter & RJ Turesky (2001) Oxidative damage and stress response from ochratoxin A exposure in rats. Free Radic Biol Med 30, 10891098.

S Knasmüller , W Parzefall & R Sanyal (1998) Use of metabolic competent human hepatoma cells for the detection of mutagens and antimutagens. Mutat Res 402, 185202.

TE Massey , RK Stewart , JM Daniels & L Liu (1995) Biochemical and molecular aspects of mammalian susceptibility to aflatoxin B 1 carcinogenicity. Proc Soc Exp Biol Med 208, 213227.

RF Omar & AD Rahimtula (1983) Possible role of an iron–oxygen complex in 4(S)-4-hydroxyochratoxin A formation by rat liver microsomes. Biochem Pharmacol 46, 20732081.

RF Omar , BB Hasinoff , F Mejilla & AD Rahimtula (1990) Mechanism of ochratoxin A stimulated lipid peroxidation. Biochem Pharmacol 40, 11831191.

S Passamonti , U Vrhovsek , A Vanzo & F Mattivi (2003) The stomach as a site for anthocyanins absorption from food. FEBS Lett 544, 210213.

PG Pietta (2000) Flavonoids as antioxidants. J Nat Prod 63, 10351042.

AD Rahimtula , JC Bereziat , V Bussacchini-Griot & H Bartsch (1988) Lipid peroxidation as a possible cause of ochratoxin A toxicity. Biochem Pharmacol 37, 44694477.

C Renzulli , F Galvano , L Pierdomenico , E Speroni & MC Guerra (2004) Effects of rosmarinic acid against aflatoxin B1 and ochratoxin A-induced cell damage in a human hepatoma cell line (Hep G2). J Appl Toxicol 24, 289296.

CA Rice-Evans , NJ Miller & G Paganga (1996) Structure–antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med 20, 933956.

AF Rizzo , F Atroshi , M Ahotupa , S Sankari & E Elovaara (1994) Protective effect of antioxidants against free radical-mediated lipid peroxidation induced by DON or T-2 toxin. J Vet Med Assoc 41, 8190.

E Speroni , R Cervellati , P Govoni , S Guizzardi , C Renzulli & MC Guerra (2003) Efficacy of different Cynara scolymus preparations on liver complaints. J Ethnopharmacol 86, 203211.

PS Steyn & MA Stander (1999) Mycotoxins as causal factors of diseases in humans. J Toxicol Toxin Rev 18, 229243.

JA Vinson , YA Dabbagh , MM Serry & J Jang (1995) Plant flavonoids, especially tea flavonols, are powerful antioxidant using an in vitro oxidation model for heart disease. J Agric Food Chem 43, 56775684.

SY Wang & AW Stretch (2001) Antioxidant capacity in cranberry is influenced by cultivar and storage temperature. J Agric Food Chem 49, 969974.

JJ Yin , MJ Smith , RM Eppley , SW Page & JA Sphon (1998) Effects of fumonisin B 1 on lipid peroxidation in membranes. Biochim Biophys Acta 1371, 134142.

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