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Dietary chia seed induced changes in hepatic transcription factors and their target lipogenic and oxidative enzyme activities in dyslipidaemic insulin-resistant rats

  • Andrea S. Rossi (a1), Maria E. Oliva (a1), Maria R. Ferreira (a1), Adriana Chicco (a1) and Yolanda B. Lombardo (a1)...
Abstract

The present study analyses the effect of dietary chia seed rich in n-3 α-linolenic acid on the mechanisms underlying dyslipidaemia and liver steatosis developed in rats fed a sucrose-rich diet (SRD) for either 3 weeks or 5 months. The key hepatic enzyme activities such as fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), glucose-6-phosphate dehydrogenase (G-6-PDH), carnitine palmitoyltransferase-1 (CPT-1) and fatty acid oxidase (FAO) involved in lipid metabolism and the protein mass levels of sterol regulatory element-binding protein-1 (SREBP-1) and PPARα were studied. (1) For 3 weeks, Wistar rats were fed either a SRD with 11 % of maize oil (MO) as dietary fat or a SRD in which chia seed replaced MO (SRD+Chia). (2) A second group of rats were fed a SRD for 3 months. Afterwards, half the rats continued with the SRD while for the other half, MO was replaced by chia for 2 months (SRD+Chia). In a control group, maize starch replaced sucrose. Liver TAG and the aforementioned parameters were analysed in all groups. The replacement of MO by chia in the SRD prevented (3 weeks) or improved/normalised (5 months) increases in dyslipidaemia, liver TAG, FAS, ACC and G-6-PDH activities, and increased FAO and CPT-1 activities. Protein levels of PPARα increased, and the increased mature form of SREBP-1 protein levels in the SRD was normalised by chia in both protocols (1 and 2). The present study provides new data regarding some key mechanisms related to the fate of hepatic fatty acid metabolism that seem to be involved in the effect of dietary chia seed in preventing and normalising/improving dyslipidaemia and liver steatosis in an insulin-resistant rat model.

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      Dietary chia seed induced changes in hepatic transcription factors and their target lipogenic and oxidative enzyme activities in dyslipidaemic insulin-resistant rats
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      Dietary chia seed induced changes in hepatic transcription factors and their target lipogenic and oxidative enzyme activities in dyslipidaemic insulin-resistant rats
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Corresponding author
*Corresponding author: Professor Dr Y. B. Lombardo, fax +54 342 4575221, email ylombard@fbcb.unl.edu.ar
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3 YB Lombardo & AG Chicco (2006) Effects of dietary polyunsaturated n-3 fatty acids on dyslipidemia and insulin resistance in rodents and humans. A review. J Nutr Biochem 17, 113.

5 D Mozaffarian , A Ascherio , FB Hu , et al. (2005) Interplay between different polyunsaturated fatty acids and risk of coronary heart disease in men. Circulation 111, 157164.

6 L Djousse , DK Arnett , JJ Carr , et al. (2005) Dietary linolenic acid is inversely associated with calcified atherosclerotic plaque in the coronary arteries: the National Heart, Lung, and Blood Institute Family Heart Study. Circulation 111, 29212926.

7 HK Kim & H Choi (2001) Dietary alpha-linolenic acid lowers postprandial lipid levels with increase of eicosapentaenoic and docosahexaenoic acid contents in rat hepatic membrane. Lipids 36, 13311336.

8 ML Garg , E Sebokova , A Wierzbicki , et al. (1988) Differential effects of dietary linoleic and alpha-linolenic acid on lipid metabolism in rat tissues. Lipids 23, 847852.

9 Ghafoorunissa , A Ibrahim & S Natarajan (2005) Substituting dietary linoleic acid with alpha-linolenic acid improves insulin sensitivity in sucrose fed rats. Biochim Biophys Acta 1733, 6775.

10 CW Weber , HS Gentry , EA Kohlhepp , et al. (1991) The nutritional and chemical evaluation of chia seeds. Ecol Foods Nutr 26, 119125.

11 AA Bushway , AM Wilson , L Houston , et al. (1984) Selected properties of the lipid and protein fractions from chia seed. J Food Sci 49, 555557.

12 R Ayerza Jr & W Coates (2007) Effect of dietary alpha-linolenic fatty acid derived from chia when fed as ground seed, whole seed and oil on lipid content and fatty acid composition of rat plasma. Ann Nutr Metab 51, 2734.

14 YB Lombardo , S Drago , A Chicco , et al. (1996) Long-term administration of a sucrose-rich diet to normal rats: relationship between metabolic and hormonal profiles and morphological changes in the endocrine pancreas. Metabolism 45, 15271532.

16 H Poudyal , SK Panchal , J Waanders , et al. (2012) Lipid redistribution by α-linolenic acid-rich chia seed inhibits stearoyl-CoA desaturase-1 and induces cardiac and hepatic protection in diet-induced obese rats. J Nutr Biochem 23, 153162.

17 AS Rossi , YB Lombardo , JM Lacorte , et al. (2005) Dietary fish oil positively regulates plasma leptin and adiponectin levels in sucrose-fed, insulin-resistant rats. Am J Physiol Regul Integr Comp Physiol 289, R486R494.

18 GJ Hein , AM Bernasconi , MA Montanaro , et al. (2010) Nuclear receptors and hepatic lipidogenic enzyme response to a dyslipidemic sucrose-rich diet and its reversal by fish oil n-3 polyunsaturated fatty acids. Am J Physiol Endocrinol Metab 298, E429E439.

19 R Zimmermann , G Haemmerle , EM Wagner , et al. (2003) Decreased fatty acid esterification compensates for the reduced lipolytic activity in hormone-sensitive lipase-deficient white adipose tissue. J Lipid Res 44, 20892099.

21 T Nakatani , HJ Kim , Y Kaburagi , et al. (2003) A low fish oil inhibits SREBP-1 proteolytic cascade, while a high-fish-oil feeding decreases SREBP-1 mRNA in mice liver: relationship to anti-obesity. J Lipid Res 44, 369379.

24 A Vrana , L Kazdova , Z Dobesova , et al. (1993) Triglyceridemia, glucoregulation, and blood pressure in various rat strains. Effects of dietary carbohydrates. Ann N Y Acad Sci 683, 5768.

25 N Yahagi , H Shimano , AH Hasty , et al. (1999) A crucial role of sterol regulatory element-binding protein-1 in the regulation of lipogenic gene expression by polyunsaturated fatty acids. J Biol Chem 274, 3584035844.

26 I Shimomura , H Shimano , BS Korn , et al. (1998) Nuclear sterol regulatory element-binding proteins activate genes responsible for the entire program of unsaturated fatty acid biosynthesis in transgenic mouse liver. J Biol Chem 273, 3529935306.

27 Y Nagai , Y Nishio , T Nakamura , et al. (2002) Amelioration of high fructose-induced metabolic derangements by activation of PPARα. Am J Physiol Endocrinol Metab 282, E1180E1190.

29 JP Pegorier (1998) Regulation of gene expression by fatty acids. Curr Opin Clin Nutr Metab Care 1, 329334.

30 HK Kim (2004) Suppression of hepatic fatty acid synthase by feeding alpha-linolenic acid rich perilla oil lowers plasma triacylglycerol level in rats. J Nutr Biochem 15, 485492.

31 P Benatti , G Peluso , R Nicolai , et al. (2004) Polyunsaturated fatty acids: biochemical, nutritional and epigenetic properties. J Am Coll Nutr 23, 281302.

32 NM Jeffery , EA Newsholme & PC Calder (1997) Level of polyunsaturated fatty acids and the n-6 to n-3 polyunsaturated fatty acid ratio in the rat diet alter serum lipid levels and lymphocyte functions. Prostaglandins Leukot Essent Fatty Acids 57, 149160.

33 S Neschen , I Moore , W Regittnig , et al. (2002) Contrasting effects of fish oil and safflower oil on hepatic peroxisomal and tissue lipid content. Am J Physiol Endocrinol Metab 282, E395E401.

34 T Ide , H Kobayashi , L Ashakumary , et al. (2000) Comparative effects of perilla and fish oils on the activity and gene expression of fatty acid oxidation enzymes in rat liver. Biochim Biophys Acta 1485, 2335.

35 T Ide (2000) Effects of dietary alpha-linolenic acid on the activity and gene expression of hepatic fatty acid oxidation enzymes. Biofactors 13, 914.

38 V Vuksan , AL Jenkins , AG Dias , et al. (2010) Reduction in postprandial glucose excursion and prolongation of satiety: possible explanation of the long-term effects of whole grain Salba (Salvia hispanica L.). Eur J Clin Nutr 64, 436438.

39 V Vuksan , D Whitham , JL Sievenpiper , et al. (2007) Supplementation of conventional therapy with the novel grain Salba (Salvia hispanica L.) improves major and emerging cardiovascular risk factors in type 2 diabetes: results of a randomized controlled trial. Diabetes Care 30, 28042810.

40 T Muramatsu , H Yatsuya , H Toyoshima , et al. (2010) Higher dietary intake of alpha-linolenic acid is associated with lower insulin resistance in middle-aged Japanese. Prev Med 50, 272276.

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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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