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Dietary fish oil reduces skeletal muscle oxygen consumption, provides fatigue resistance and improves contractile recovery in the rat in vivo hindlimb

  • Gregory E. Peoples (a1) and Peter L. McLennan (a2)
  • DOI:
  • Published online: 09 August 2010

Dietary fish oil modulates skeletal muscle membrane fatty acid composition. Similar changes in heart membrane composition modulate myocardial oxygen consumption and enhance mechanical performance. The rat in vivo autologous perfused hindlimb was used to investigate the influence of membrane composition on skeletal muscle function. Male Wistar rats were fed either saturated fat (SF), n-6 PUFA (linoleic acid rich) or n-3 PUFA (fish oil) diets for 8 weeks. Hindlimb skeletal muscle perfused using the animal's own blood was stimulated via the sciatic nerve (1 Hz, 6-12 V, 0·05 ms) to contract in repeated 10 min bouts. The n-3 PUFA diet markedly increased 22 : 6n-3 DHA, total n-3 PUFA and decreased the n-6:n-3 PUFA ratio (P < 0·05) in red and white skeletal muscle membranes. There was no difference in initial twitch tension but the n-3 PUFA group maintained greater twitch tension within all contraction bouts and recovered better during rest to produce greater twitch tension throughout the final contraction bout (P < 0·05). Hindlimb oxygen consumption during contraction was significantly lower in the n-3 PUFA group compared with the SF group, producing a significantly higher O2 efficiency index compared with both SF and n-6 PUFA groups (P < 0·05). Resting oxygen consumption was increased in recovery in the SF group (P < 0·05) but did not change in the n-3 PUFA group. Membrane incorporation of n-3 PUFA DHA following fish oil feeding was associated with increased efficiency of muscle O2 consumption and promoted resistance to muscle fatigue.

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*Corresponding author: Dr G. E. Peoples, fax +61 2 4221 3486, email
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1R Gorjao , A Azevedo-Martins , H Rodrigues , (2009) Comparative effects of DHA and EPA on cell function. Pharmacol Therapeut 122, 5664.

2R Siddiqui , K Harvey & G Zaloga (2008) Modulation of enzymatic activities by n-3 polyunsaturated fatty acids to support cardiovascular health. J Nutr Biochem 19, 417437.

3P McLennan , P Howe , M Abeywardena , (1996) The cardiovascular protective role of docosahexaenoic acid. Eur J Pharmacol 300, 8389.

4P McLennan , MY Abeywardena & JS Charnock (1988) Dietary fish oil prevents ventricular fibrillation following coronary artery occlusion and reperfusion. Am Heart J 116, 709717.

13DA Pan , S Lillioja , MR Milner , (1995) Skeletal muscle membrane lipid composition is related to adiposity and insulin action. J Clin Invest 96, 28022808.

14PL McLennan , LR Barnden , MY Abeywardena , (1989) Dietary polyunsaturated fatty acids improve cardiac function in the marmoset monkey. J Mol Cell Cardiol 21, 32.

15JS Charnock , PL McLennan , BR McIntosh , (1987) Radionuclide angio-graphic study of the influence of the dietary lipid supplements on the cardiac function in the marmoset (Callithrix jacchus). Cardiovasc Res 21, 369376.

17S Pepe & PL McLennan (2002) Cardiac membrane fatty acid composition modulates myocardial oxygen consumption and postischemic recovery of contractile function. Circulation 105, 23032308.

18C O'Connor , L Lawrence , A Lawrence , (2004) The effect of dietary fish oil supplementation on exercising horses. J Anim Sci 82, 29782984.

19PL McLennan , TM Bridle & M Abeywardena (1992) Dietary lipid modulation of ventricular fibrillation threshold in the marmoset monkey. Am Heart J 123, 15551561.

21J Dallongeville , J Yarnell , P Ducimetiere , (2003) Fish consumption is associated with lower heart rates. Circulation 108, 820825.

22A Shah , A Ichiuji , J Han , (2007) Cardiovascular and endothelial effects of fish oil supplementation in health volunteers. J Cardiovasc Pharmacol Theraput 12, 213219.

23G Peoples , P McLennan , P Howe , (2008) Fish oil reduces heart rate and oxygen consumption during exercise. J Cardiovasc Pharmacol 52, 540547.

24D Mozaffarian , A Geelen , I Brouwer , (2005) Effect of fish oil on heart rate - A meta-analysis of randomised controlled trials. Circulation 112, 19451952.

26A Owen , B Peter-Przyborowska , A Hoy , (2004) Dietary fish oil dose- and time-response effects on cardiac phospholipid fatty acid composition. Lipids 39, 955961.

29LH Storlien , LA Baur , AD Kriketos , (1996) Dietary fats and insulin action. Diabetologia 39, 621631.

31B Vessby , S Tengblad & H Lithell (1994) Insulin sensitivity is realted to the fatty acid composition in serum lipids and skeletal muscle phospholipids in 70 year old men. Diabetologia 37, 10441050.

35KJ Ayre & AJ Hulbert (1997) Dietary fatty acid profile effects endurance in rats. Lipids 32, 12651270.

36S Zhang , J Bruton , A Katz , (2006) Limited oxygen diffusion accelerates fatigue development in mouse skeletal muscle. J Physiol 572, 551559.

37L Demaison , J Blet , JP Sergiel , (2000) Effect of dietary polyunsaturated fatty acids on contractile function of hearts isolated from sedentary and trained rats. Reprod Nutr Dev 40, 113125.

39P McLennan & M Abeywardena (2005) Membrane basis for fish oil effects on the heart: linking natural hibernators to prevention of human sudden cardiac death. J Membr Biol 206, 85102.

42L Ferreira & M Reid (2008) Muscle-derived ROS and thiol regulation in muscle fatigue. J Appl Physiol 104, 853860.

43S al Makdessi , M Brandle , M Ehrt , (1995) Myocardial protection by ischemic preconditioning: the influence of the composition of myocardial phospholipids. Mol Cell Biochem 145, 6973.

44O Pansarasa , G D'Antona , M Gualea , (2002) ‘‘Oxidative stress’’: effects of mild endurance training and testosterone treatment on rat gastrocnemius muscle. Eur J Appl Physiol 87, 550555.

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