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Dietary protein intake affects expression of genes for lipid metabolism in porcine skeletal muscle in a genotype-dependent manner

  • Yingying Liu (a1) (a2) (a3), Fengna Li (a1), Lingyun He (a1) (a4), Bie Tan (a1), Jinping Deng (a4), Xiangfeng Kong (a1), Yinghui Li (a1) (a3), Meimei Geng (a1), Yulong Yin (a1) (a5) and Guoyao Wu (a1) (a6)...

Skeletal muscle is a major site for the oxidation of fatty acids (FA) in mammals, including humans. Using a swine model, we tested the hypothesis that dietary protein intake regulates the expression of key genes for lipid metabolism in skeletal muscle. A total of ninety-six barrows (forty-eight pure-bred Bama mini-pigs (fatty genotype) and forty-eight Landrace pigs (lean genotype)) were fed from 5 weeks of age to market weight. Pigs of fatty or lean genotype were randomly assigned to one of two dietary treatments (low- or adequate-protein diet), with twenty-four individually fed pigs per treatment. Our data showed that dietary protein levels affected the expression of genes involved in the anabolism and catabolism of lipids in the longissimus dorsi and biceps femoris muscles in a genotype-dependent manner. Specifically, Bama mini-pigs had more intramuscular fat, SFA and MUFA, as well as elevated mRNA expression levels of lipogenic genes, compared with Landrace pigs. In contrast, Bama mini-pigs had lower mRNA expression levels of lipolytic genes than Landrace pigs fed an adequate-protein diet in the growing phase. These data are consistent with higher white-fat deposition in Bama mini-pigs than in Landrace pigs. In conclusion, adequate provision of dietary protein (amino acids) plays an important role in regulating the expression of key lipogenic genes, and the growth of white adipose tissue, in a genotype- and tissue-specific manner. These findings have important implications for developing novel dietary strategies in pig production.

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* Corresponding authors: Dr X. Kong, fax +86 731 84612685, email; Dr Y. Yin, fax +86 731 84612685, email
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1 Wu, GY (2013) Functional amino acids in nutrition and health. Amino Acids 45, 407411.
2 Duan, Y, Li, FN, Li, LL, et al. (2014) n-6:n-3 PUFA ratio is involved in regulating lipid metabolism and inflammation in pigs. Br J Nutr 111, 445451.
3 Li, FN, Duan, YF, Li, YF, et al. (2015) Effects of dietary n-6:n-3 PUFA ratio on fatty acid composition, free amino acid profile and gene expression of transporters in finishing pigs. Br J Nutr (In the Press).
4 Wood, JD, Nute, GR, Richardson, RI, et al. (2004) Effects of breed, diet and muscle on fat deposition and eating quality in pigs. Meat Sci 67, 651667.
5 Wu, GY, Fanzo, J, Miller, DD, et al. (2014) Production and supply of high-quality food protein for human consumption: sustainability, challenges and innovations. Ann N Y Acad Sci 1, 119.
6 Blanchard, PJ, Ellis, M, Warkup, CC, et al. (1999) The influence of rate of lean and fat tissue development on pork eating quality. Anim Sci 68, 477485.
7 Yang, HS & Yin, YL (2012) Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigs. Molec Cell Endocrinol 365, 4451.
8 Wood, JD, Enser, M, Fisher, AV, et al. (2008) Fat deposition, fatty acid composition and meat quality: a review. Meat Sci 78, 343358.
9 Li, FN, Yang, HS, Duan, Y, et al. (2011) Myostatin regulates preadipocyte differentiation and lipid metabolism of adipocyte via ERK1/2. Cell Biol Int 35, 11411146.
10 Li, FN, Li, LL & Yang, Y (2011) Regulation of soy isoflavones on weight gain and fat percentage: evaluation in a Chinese minipig model. Animal 5, 19031908.
11 Mathis, N, Feidt, C & Brun-Bellut, J (2003) Influence of protein/energy ratio on carcass quality during the growing period of Eurasian perch (Perca fluviatilis). Aquaculture 217, 453464.
12 Noakes, M, Keogh, JB, Foster, PR, et al. (2005) Effect of an energy-restricted, high-protein, low-fat diet relative to a conventional high-carbohydrate, low-fat diet on weight loss, body composition, nutritional status, and markers of cardiovascular health in obese women. Am J Clin Nutr 81, 12981306.
13 Barea, R, Nieto, R & Aguilera, JF (2007) Effects of the dietary protein content and the feeding level on protein and energy metabolism in Iberian pigs growing from 50 to 100 kg body weight. Animal 1, 357365.
14 Teye, GA, Sheard, PR, Whittington, FM, et al. (2006) Influence of dietary oils and protein level on pork quality. 1. Effects on muscle fatty acid composition, carcass, meat and eating quality. Meat Sci 73, 157165.
15 Kawaguchi, H, Miyoshi, N, Miura, N, et al. (2011) Microminipig, a non-rodent experimental animal optimized for life science research: novel atherosclerosis model induced by high fat and cholesterol diet. J Pharmacol Sci 115, 115121.
16 Yang, HS, Fu, FZ, Shao, H, et al. (2012) Impacts of birth weight on plasma, liver and skeletal muscle neutral amino acid profiles and intestinal amino acid transporters in suckling Huanjiang mini-piglets. PLOS ONE 7, e50921.
17 National Research Council (NRC) (2012) Nutrient Requirements of Swine. Washington, DC: National Academy Press.
18 Ministry of Agriculture of the People's Republic of China (2004) Feeding Standard of Swine (GB, NY/T 65-2004). Beijing: China Agriculture Press.
19 Yin, J, Ren, WK, Duan, J, et al. (2014) Dietary arginine supplementation enhances intestinal expression of SLC7A7 and SLC7A1 and ameliorates growth depression in mycotoxin-challenged pigs. Amino Acids 46, 883892.
20 Kong, XF, Wu, GY, Liao, YP, et al. (2007) Dietary supplementation with Chinese herbal ultra-fine powder enhances cellular and humoral immunity in early-weaned piglets. Livest Sci 108, 9498.
21 Yin, FG, Liu, YL, Yin, YL, et al. (2009) Dietary supplementation with Astragalus polysaccharide enhances ileal digestibilities and serum concentrations of amino acids in early weaned piglets. Amino Acids 37, 263270.
22 Zhou, XL, Kong, XF, Lian, GQ, et al. (2014) Dietary supplementation with soybean oligosaccharides increases short-chain fatty acids but decreases protein-derived catabolites in the intestinal luminal content of weaned Huanjiang mini-piglets. Nutr Res 34, 780788.
23 Kang, P, Zhang, L, Hou, Y, et al. (2014) Effects of l-proline on the growth performance, and blood parameters in weaned lipopolysaccharide (CPS)-challenged pigs. Asian-Aust J Anim Sci 27, 11501156.
24 Ren, WK, Yin, J, Wu, MM, et al. (2014) Effects of l-arginine or l-glutamine supplementation in dextran sulfate sodium colitis. PLOS ONE 9, e88335.
25 Feng, ZM, Zhou, XL, Wu, F, et al. (2014) Both dietary supplementation with monosodium l-glutamate and fat modify circulating and tissue amino acid pools in growing pigs, but with little interactive effect. PLOS ONE 9, e84533.
26 Ministry of Agriculture of the People's Republic of China (1987) Performance Measurement Technology Regulations for Chinese Lean Pig. Beijing: China Standard Press.
27 Cunniff, P (editor) (1997) Official Methods of Analysis of AOAC (Association of Official Analytical Chemists) International, 16th ed. Gaithersburg, MD: AOAC.
28 Tan, B, Yin, YL, Liu, ZQ, et al. (2009) Dietary l-arginine supplementation increases muscle gain and reduces body fat mass in growing–finishing pigs. Amino Acids 37, 169175.
29 Yin, YL, McEvoy, J, Souffrant, WB, et al. (2000) Apparent digestibility (ileal and overall) of nutrients and endogenous nitrogen losses in growing pigs fed wheat or wheat by-products without or with xylanase supplementation. Livest Prod Sci 62, 119132.
30 Wu, X, Ruan, Z, Gao, YL, et al. (2010) Dietary supplementation with l-arginine or N-carbamylglutamate enhances intestinal growth and heat shock protein-70 expression in weanling pigs fed a corn- and soybean meal-based diet. Amino Acids 39, 831839.
31 Wang, W, Blachier, F, Fu, D, et al. (2013) Ontogenic expression of the amino acid transporter b0,1AT in suckling Huanjiang piglets: effect of intra-uterine growth restriction. Br J Nutr 110, 823830.
32 Liu, XD, Wu, X, Yin, YL, et al. (2012) Effects of dietary l-arginine or N-carbamylglutamate supplementation during late gestation of sows on the miR-15b/16, miR-221/222, VEGFA and eNOS expression in umbilical vein. Amino Acids 42, 21112119.
33 Bustin, SA, Benes, V, Garson, JA, et al. (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55, 611622.
34 Wang, W, Shi, CY, Zhang, JS, et al. (2009) Molecular cloning, distribution and ontogenetic expression of the oligopeptide transporter PepT1 mRNA in Tibetan suckling piglets. Amino Acids 37, 593601.
35 Sink, JD, Wilson, LL, McCarthy, RD, et al. (1973) Interrelationships between serum-lipids, energy intake, milk-production, growth and body characteristics in Angus–Holstein cows and their progeny. J Anim Sci 36, 313317.
36 Anderson, JW & Konz, EC (2001) Obesity and disease management: effects of weight loss on comorbid conditions. Obes Res 9, 326S334S.
37 Cameron, ND, Enser, M, Nute, GR, et al. (2000) Genotype with nutrition interaction on fatty acid composition of intramuscular fat and the relationship with flavour of pig meat. Meat Sci 55, 187195.
38 Munday, MR (2002) Regulation of mammalian acetyl-CoA carboxylase. Biochem Soc Trans 30, 10591064.
39 Chen, J, Yang, XJ, Tong, H, et al. (2004) Expressions of FAS and HSL mRNA in longissimus dorsi muscle and their relation to intramuscular fat contents in pig. J Agric Biotechnol 12, 422426.
40 Tan, B, Yin, YL, Liu, ZQ, et al. (2011) Dietary l-arginine supplementation differentially regulates expression of lipid-metabolic genes in porcine adipose tissue and skeletal muscle. J Nutr Biochem 22, 441445.
41 Loftus, TM, Jaworsky, DE, Frehywot, GL, et al. (2000) Reduced food intake and body weight in mice treated with fatty acid synthase inhibitors. Science 288, 23792381.
42 Zechner, R (1997) The tissue-specific expression of lipoprotein lipase: implications for energy and lipoprotein metabolism. Curr Opin Lipidol 8, 7788.
43 Zou, CH & Shao, JH (2008) Role of adipocytokines in obesity-associated insulin resistance. J Nutr Biochem 19, 277286.
44 Binnert, C, Koistinen, HA, Martin, GV, et al. (2000) Fatty acid transport protein-1 mRNA expression in skeletal muscle and in adipose tissue in humans. Am J Physiol Endocrinol Metab 279, E1072E1079.
45 Bonen, A, Chabowski, A, Luiken, J, et al. (2007) Mechanisms and regulation of protein-mediated cellular fatty acid uptake: molecular, biochemical, and physiological evidence. Physiology 22, 1529.
46 DiRusso, CC, Li, H, Darwis, D, et al. (2005) Comparative biochemical studies of the murine fatty acid transport proteins (FATP) expressed in yeast. J Biol Chem 280, 1682916837.
47 Man, MZ, Hui, TY, Schaffer, JE, et al. (1996) Regulation of the murine adipocyte fatty acid transporter gene by insulin. Mol Endocrinol 10, 10211028.
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