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Dietary plasma proteins attenuate the innate immunity response in a mouse model of acute lung injury

  • Mònica Maijó (a1), Lluïsa Miró (a1), Javier Polo (a2), Joy Campbell (a3), Louis Russell (a3), Joe Crenshaw (a3), Eric Weaver (a4), Miquel Moretó (a1) and Anna Pérez-Bosque (a1) (a2)...
Abstract

We examined whether oral plasma protein supplements affect the innate immune response in a model of acute lung inflammation. Mice were fed diets supplemented with 8 % spray-dried plasma (SDP) or 2 % plasma Ig concentrate (IC) from day 19 (weaning) until day 34. The mice were challenged with intranasal lipopolysaccharide (LPS) at day 33 (and killed 24 h later for cytokine and leucocyte analyses) or at day 34 (and killed 6 h later for cytokine determinations). In bronchoalveolar lavage fluid (BALF), LPS increased the number of leucocytes by twenty-sevenfold, an effect that was partly prevented by both SDP and IC, and by twentyfold the percentage of activated monocytes, which was partly prevented by SDP. In the lung tissue, LPS increased the infiltrated leucocytes, and this effect was prevented in part by SDP. In unchallenged mice, both SDP and IC diets reduced the percentage of resident neutrophils and monocytes (P < 0·05). In the blood, both SDP and IC completely prevented LPS-dependent monocyte activation (CD14+; P < 0·05). LPS dramatically increased the concentration of cytokines (TNF-α, IL-1α, IL-6, granulocyte-macrophage colony-stimulating factor and granulocyte colony-stimulating factor) and chemokines (CXCL1, CCL2, CCL3 and CCL4) in BALF. The acute response of cytokine production was reduced by 20–80 % by both SDP and IC. For chemokines, plasma supplements had no effect on LPS-induced CXCL1 expression but significantly reduced CCL2, CCL3 and CCL4 production (P < 0·05). The results support the view that dietary plasma proteins can be used to attenuate endotoxin-associated lung inflammation.

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Corresponding author
*Corresponding author: Dr A. Pérez-Bosque, fax +34 934035901, email anna.perez@ub.edu
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1 LA Augusto , J Li , M Synguelakis , (2002) Structural basis for interactions between lung surfactant protein C and bacterial lipopolysaccharide. J Biol Chem 277, 2348423492.

2 N Chaudhuri & I Sabroe (2008) Basic science of the innate immune system and the lung. Paediatr Respir Rev 9, 236242.

7 A Pontes-Arruda , S Demichele , A Seth , (2008) The use of an inflammation-modulating diet in patients with acute lung injury or acute respiratory distress syndrome: a meta-analysis of outcome data. JPEN J Parenter Enteral Nutr 32, 596605.

8 YC Xie , XW Dong , XM Wu , (2009) Inhibitory effects of flavonoids extracted from licorice on lipopolysaccharide-induced acute pulmonary inflammation in mice. Int Immunopharmacol 9, 194200.

11 H Kiyono & S Fukuyama (2004) NALT- versus Peyer's patch-mediated mucosal immunity. Nat Rev Immunol 4, 699710.

19 JA Carroll , KJ Touchette , RL Matteri , (2002) Effect of spray-dried plasma and lipopolysaccharide exposure on weaned pigs: II. Effects on the hypothalamic–pituitary–adrenal axis of weaned pigs. J Anim Sci 80, 502509.

26 A Pérez-Bosque , L Miró , J Polo , (2010) Dietary plasma protein supplements prevent the release of mucosal proinflammatory mediators in intestinal inflammation in rats. J Nutr 140, 2530.

27 JM Campbell , J Polo , LE Russell , (2010) Review of spray-dried plasma's impact on intestinal barrier function. Livest Sci 133, 239241.

34 K Schuh & A Pah (2009) Inhibition of the MAP kinase ERK protects from lipopolysaccharide-induced lung injury. Biochem Pharmacol 77, 18271834.

36 R Larsson , D Rocksén , B Lilliehöök , (2000) Dose-dependent activation of lymphocytes in endotoxin-induced airway inflammation. Infect Immun 68, 69626969.

37 R Puljic , E Benediktus , C Plater-Zyberk , (2007) Lipopolysaccharide-induced lung inflammation is inhibited by neutralization of GM-CSF. Eur J Pharmacol 557, 230235.

40 NL Anderson & NG Anderson (2002) The human plasma proteome: history, character, and diagnostic prospects. Mol Cell Proteomics 1, 845867.

41 SK Bhandari , B Xu , CM Nyachoti , (2008) Evaluation of alternatives to antibiotics using an Escherichia coli K88+ model piglet diarrhea: effects on gut microbial ecology. J Anim Sci 86, 836847.

44 JR Heyen , S Ye , BN Finck , (2000) Interleukin (IL)-10 inhibits IL-6 production in microglia by preventing activation of NF-kappaB. Brain Res Mol Brain Res 77, 138147.

45 D Ogando , M Cella , ML Ribeiro , (2004) IL-10 inhibits nitric oxide synthesis in murine uterus. Neuroimmunomodulation 11, 127132.

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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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