Skip to main content Accessibility help
×
×
Home

Difference in the metabolism of vitamin K between liver and bone in vitamin K-deficient rats

  • Toshiro Sato (a1), Yutaka Ohtani (a1), Yoko Yamada (a1), Sanshiroh Saitoh (a2) and Hiroshi Harada (a3)...

Abstract

The difference between vitamin K metabolism in the liver and that in the bone of vitamin K-deficient rats was examined. After 17 d administration of vitamin K-deficient food, vitamin K in the liver was almost depleted, and prothrombin time (PT) was prolonged. Serum total osteocalcin level was slightly decreased by vitamin K deficiency, whereas serum undercarboxylated osteocalcin level did not change. The level of menaquinone (MK)-4 as well as that of phylloquinone was decreased, but approximately 40 % of the initial level still existed in the femur after the 17 d period. A single-dose administration of vitamin K (250 nmol/kg body weight) markedly increased vitamin K level in the liver but not in the femur. These results suggest that the turnover of vitamin K in the bone is slower than that in the liver, and bone metabolism may be little affected by the short period of intake of vitamin K-deficient food. However, intake of a larger amount of vitamin K is required for its accumulation in the bone than in the liver. Furthermore, the counteracting effect of MK-7 on prolonged PT in vitamin K-deficient rats was found to be higher than phylloquinone or MK-4.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Difference in the metabolism of vitamin K between liver and bone in vitamin K-deficient rats
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Difference in the metabolism of vitamin K between liver and bone in vitamin K-deficient rats
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Difference in the metabolism of vitamin K between liver and bone in vitamin K-deficient rats
      Available formats
      ×

Copyright

Corresponding author

*Corresponding author: Dr T. Sato, fax +81 538 23 6772, email tosato@honen.co.jp

References

Hide All
Akiyama, Y, Hara, K, Matsumoto, A, Takahara, S & Tajima, T (1995) Comparison of intestinal absorption of vitamin K2 (menaquinone) homologues and their effects on blood coagulation in rats with hypoprothrombinaemia. Biochemical Pharmacology 49, 18011807.
Barns, RH & Fiala, G (1959) Effects of prevention of coprophagy in the rat. VI. Vitamin K1. Journal of Nutrition 68, 603614.
Booth, SL & Suttie, JW (1998) Dietary intake and adequacy of vitamin K. Journal of Nutrition 128, 785788.
Collins, MD & Jones, D (1981) Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications. Microbiological Review 45, 316354.
Conly, JM & Stein, K (1992) Quantitative and qualitative measurements of K vitamins in human intestinal contents. American Journal of Gastroenterology 87, 311316.
Craciun, AM, Groenen-van Dooren, MMCL, Thijssen, HHW & Vermeer, C (1998) Induction of prothrombin synthesis by K-vitamins compared in vitamin K-deficient and in brodifacoum-treated rats. Biochimica et Biophysica Acta 1380, 7581.
Davidson, RT, Foley, AL, Engelle, JA & Suttie, JW (1998) Conversion of dietary phylloquinone to tissue menaquinone-4 in rats is not dependent on gut bacteria. Journal of Nutrition 128, 220223.
Groenen-van Dooren, MMCL, Ronden, JE, Soute, BAM & Vermeer, C (1993) The relative effects of phylloquinone and menaquinone on the blood coagulation factor synthesis in vitamin K-deficient rats. Biochemical Pharmacology 50, 797801.
Groenen-van Dooren, MMCL, Ronden, JE, Soute, BAM & Vermeer, C (1995) Bioavailability of phylloquinone and menaquinones after oral and colorectal administration in vitamin K-deficient rats. Biochemical Pharmacology 50, 797801.
Haffa, A, Krueger, D, Bruner, J, Engelke, J, Gundberg, C, Akhter, M & Binkley, N (2000) Diet- or warfarin-induced vitamin K insufficiency elevates circulating undercarboxylated osteocalcin without altering skeletal status in growing female rats. Journal of Bone and Mineral Research 15, 872878.
Hara, K, Akiyama, Y, Nakamura, T, Murata, S & Morita, I (1995) The inhibitory effect of vitamin K2 (menatetrenone) on bone resorption may be related to its side chain. Bone 16, 179184.
Hirauchi, K, Sakano, T, Notsumoto, S, Nagaoka, T, Morimoto, A, Fujimoto, K, Masuda, S & Suzuki, Y (1989) Measurement of K vitamins in foods by high-performance liquid chromatography with fluorometric detection. Vitamins (Japan) 63, 147151.
Hodges, SJ, Akesson, K, Vergnaud, P, Obrant, K & Delmas, PD (1993 a) Circulating levels of vitamins K1 and K2 decreased in elderly women with hip fracture. Journal Bone and Mineral Research 8, 12411245.
Hodges, SJ, Bejui, J, Leclercq, M & Delmas, PD (1993 b) Detection and measurement of vitamins K1 and K2 in human cortical and trabecular bone. Journal of Bone and Mineral Research 8, 10051008.
Kaneki, M, Mizuno, Y, Hosoi, T, Inoue, S, Hoshino, S, Akishita, M, Akedo, Y, Horiki, K, Ouchi, H & Orimo, H (1995) Taikouki-kotsusosyousyou niokeru kessei vitamin K noudo no kentou (Serum concentration of vitamin K in elderly women with involutional osteoporosis). Nihon Ronen Igaku Zasshi 32, 195200.
Koshihara, Y, Hoshi, K, Ishibashi, H & Shiraki, M (1996) Vitamin K2 promotes 1α,25(OH)2 vitamin D3-induced mineralization in human periosteal osteoblasts. Calcified Tissue International 59, 466473.
Koshihara, Y, Hoshi, K & Shiraki, M (1992) Enhancement of mineralization of human osteoblasts by vitamin K2 (menatetrenone). Journal of Clinical and Experimental Medicine 161, 439440.
Mathers, JC, Fernandes, F, Hill, MJ, McCarthy, PT, Shearer, MJ & Oxley, A (1990) Dietary modification of potential vitamin K supply from enteric bacterial menaquinones in rats. British Journal of Nutrition 63, 639652.
Merle, B & Delmas, PD (1990) Normal carboxylation of circulating osteocalcin (bone Gla protein) in Paget's disease of bone. Bone and Mineral 11, 237245.
Nakanishi, T, Furukawa, M, Okuda, H, Iizuka, B & Obata, H (1989) Comparison of effects of menaquinone-4 and menaquinone-7 assessed by decrease of PIVKA-II levels in human hepatoma cells in culture. Vitamins (Japan) 63, 253257.
Poser, JW & Price, PA (1978) A method for decarboxylation of γ-carboxyglutamic acid in proteins. Journal of Biological Chemistry 254, 431436.
Price, PA (1985) Vitamin K-dependent formation of bone gla protein (osteocalcin) and its function. Vitamins and Hormones 42, 65108.
Ronden, JE, Drittij-Reijnders, MJ, Vermeer, C & Thijssen, HH (1998) Intestinal flora is not an intermediate in the phylloquinone–menaquinone-4 conversion in the rat. Biochimica et Biophysica Acta 1379, 6975.
Sakano, T, Notsumoto, S, Nagaoka, T, Morimoto, A, Fujimoto, K, Masuda, S, Suzuki, Y & Hirauchi, K (1998) Measurement of K vitamins in food by high performance liquid chromatography with fluorometric detection. Vitamins (Japan) 62, 393398.
Sato, T, Yamada, Y, Ohtani, Y, Mitsui, N, Murasawa, H & Araki, S (2001) Production of menaquinone (vitamin K2)-7 by Bacillus subtilis. Journal of Bioscience and Bioengineering 91, 1621.
Shearer, MJ (1990) Vitamin K and vitamin K-dependent proteins. British Journal of Haematology 75, 156162.
Shearer, MJ, Bach, A & Kohlemeier, M (1996) Chemistry, nutritional source, tissue distribution and metabolism of vitamin K with special reference to bone health. Journal of Nutrition 126(Suppl.), 1181S1186S.
Shiraki, M, Shiraki, Y, Aoki, C & Miura, M (2000) Vitamin K2 (menatetrenone) effectively prevents fractures and sustains lumbar bone mineral density in osteoporosis. Journal of Bone and Mineral Research 15, 515521.
Sokoll, JJ, Booth, SL, O'Brien, ME, Davidson, KW, Tsaioun, KI & Sadowski, JA (1997) Changes in serum osteocalcin, plasma phylloquinone, urinary γ-carboxyglutamic acid in response to altered intakes of dietary phylloquinone in human subjects. American Journal of Clinical Nutrition 65, 779784.
Suttie, JW (1995) The importance of menaquinones in human nutrition. Annual Reviews of Nutrition 15, 399417.
Szulc, P, Arlot, M, Chapuy, M-C, Duboeuf, F, Meunier, PJ & Desmas, PD (1994) Serum undercarboxylated osteocalcin correlates with hip bone mineral density in elderly women. Journal of Bone and Mineral Research 9, 15911595.
Szulc, P, Chapuy, M-C, Meunier, PJ & Delmas, PD (1993) Serum undercarboxylated osteocalcin is a marker of the hip fracture in elderly women. Journal of Clinical Investigation 91, 17691774.
Thijssen, HHW & Drittij-Reijnders, MJ (1994) Vitamin K distribution in rat tissues: dietary phylloquinone is a source of tissue menaquinone-4. British Journal of Nutrition 72, 415425.
Tsukamoto, Y, Ichise, H, Kakuda, H & Yamaguchi, M (2000) Intake of fermented soybean (natto) increases circulating vitamin K2 (menaquinone-7) and γ-carboxylated osteocalcin concentration in normal individuals. Journal of Bone and Mineral Metabolism 18, 216222.
Uchida, K, Nomura, Y, Takase, H, Harauchi, T, Yoshizaki, T & Nakao, H (1986) Effects of vitamin K-deficient diets and fasting on blood coagulation factors in conventional and germfree rats. Japan Journal of Pharmacology 40, 115122.
Vermeer, C, Jie, K-SG & Knapen, MHJ (1995) Role of vitamin K in bone metabolism. Annual Reviews of Nutrition 15, 122.
Yamaguchi, M, Taguchi, H, Gao, YH, Igasshi, A & Tsukamoto, Y (1999) Effect of vitamin K2 (menaquinone-7) in fermented soybean (natto) on bone loss in ovariectomized rats. Journal of Bone and Mineral Metabolism 17, 2329.
Yamamoto, R, Komai, M, Kojima, K, Furukawa, Y & Kimura, S (1997) Menaquinone-4 accumulation in various tissues after an oral administration of phylloquinone in Wistar rats. Journal of Nutritional Science and Vitaminology (Tokyo) 42, 133143.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed