Skip to main content
×
Home
    • Aa
    • Aa

Effect of acute ingestion of fresh and stored lettuce (Lactuca sativa) on plasma total antioxidant capacity and antioxidant levels in human subjects

  • Mauro Serafini, Rossana Bugianesi (a1), Monica Salucci (a1), Elena Azzini (a1), Anna Raguzzini (a1) and Giuseppe Maiani (a1)...
Abstract

The present study investigated whether storage under modified-atmosphere packaging (MAP) affected the antioxidant properties of fresh lettuce (Lactuca sativa). Eleven healthy volunteers (six men, five women) consumed 250 g fresh lettuce, and blood was sampled before (0 h) and 2, 3 and 6 h after consumption. The protocol was repeated 3 d later with the same lettuce stored at 5°C under MAP conditions (O2–N2 (5:95, v/v)). Results showed that after ingestion of fresh lettuce, plasma total radical-trapping antioxidant potential (TRAP), measured as area under the curve, was significantly higher (1·3 (SEM 0·3) MMOL/L PER 6 H; P<0·05) THAN THE VALUE OBTAINED WITH MAP-STORED LETTUCE (0·1 (sem 0·2) mmol/l per 6 h). Plasma TRAP, quercetin and p-coumaric acid were significantly different from baseline values (P≤0·05) 2 and 3 h after fresh lettuce ingestion. Caffeic acid increased significantly at 3 h (P<0·05). Plasma β-carotene levels increased significantly at 6 h (P<0·05). Vitamin C concentrations (mg/l) rose from 10·9 (sem 2·0) to 12·7 (sem 3·0) (P<0·001), 12·7 (sem 2·0) (P<0·01) and 12·9 (sem 3·0) (P<0·05) at 0, 2, 3 and 6 h respectively. No changes were observed after ingestion of MAP-stored lettuce for all the measured markers. Our present results showed that ingestion of MAP-stored lettuce does not modify plasma redox status in healthy subjects. Further research is needed to develop post-harvesting techniques able to preserve the bioactive molecule content of plant food.

    • Send article to Kindle

      To send this article to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Effect of acute ingestion of fresh and stored lettuce (Lactuca sativa) on plasma total antioxidant capacity and antioxidant levels in human subjects
      Available formats
      ×
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about sending content to Dropbox.

      Effect of acute ingestion of fresh and stored lettuce (Lactuca sativa) on plasma total antioxidant capacity and antioxidant levels in human subjects
      Available formats
      ×
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about sending content to Google Drive.

      Effect of acute ingestion of fresh and stored lettuce (Lactuca sativa) on plasma total antioxidant capacity and antioxidant levels in human subjects
      Available formats
      ×
Copyright
Corresponding author
*Corresponding author: Dr Mauro Serafini, fax +39 065031592, email serafini@inran.it
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

G Block , B Patterson & A Subar (1992) Fruit, vegetables and cancer prevention: a review of the epidemiological evidence. Nutrition and Cancer 18, 129.

A Ghiselli , M Serafini , G Maiani , E Azzini & A Ferro-Luzzi (1995) A fluorescence-based method for measuring total plasma antioxidant capability. Free Radical Biology and Medicine 18, 2936.

AA Kader , D Zagory & EL Kervel (1989) Modified atmosphere packaging of fruits and vegetables. Critical Review of Food Sciences and Nutrition 28, 130.

GT McAnlis , J McEneny , J Pearce & IS Young (1999) Absorption and antioxidant effects of quercetin from onions in man. European Journal of Clinical Nutrition 53, 9296.

G Maiani , G Pappalardo , A Ferro-Luzzi , A Raguzzini , A Azzini , A Guadalaxara , M Trifero , T Frommel & S Mobarhan (1995) Accumulation of beta-carotene in normal colorectal mucosa and colonic neoplastic lesions in humans. Nutrition and Cancer 24, 2331.

G Maiani , M Serafini , M Salucci , E Azzini & A Ferro-Luzzi (1997) Application of a new high-performance liquid chromatographic method for measuring selected polyphenols in human plasma. Journal of Chromatography B 692, 311317.

ER Miller , JA Lawrence & TH Risby (1998) Effect of dietary patterns on measures of lipid peroxidation. Results from a randomized clinical trial. Circulation 98, 23902395.

J Moon , T Tsushida , K Nakahara & J Terao (2001) Identification of quercetin 3-O-β-d-glucuronide as an antioxidative metabolite in rat plasma after oral administration of quercetin. Free Radical Biology and Medicine 30, 12741285.

N Pellegrini , P Riso & M Porrini (2000) Tomato consumption does not affect the total antioxidant capacity of plasma. Nutrition 16, 268271.

WD Powrie & BJ Skura (1991) Modified Atmosphere Packaging of Fruit and Vegetables. Chichester, Sussex: Ellis Horwood.

M Richelle , I Tavazzi , M Enslen & EA Offord (1999) Plasma kinetics in man of epicatechin from black chocolate. European Journal of Clinical Nutrition 53, 2226.

T Swain & E Hillis (1959) The phenolic constituents of Prunus domestica. The quantitative analysis of phenolic constituents. Journal of Science and Food Agriculture 10, 6368.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords: