Skip to main content

Tomatoes have natural anti-thrombotic effects

  • J. Yamamoto (a1), T. Taka (a1), K. Yamada (a1), Y. Ijiri (a1), M. Murakami (a1), Y. Hirata (a1), A. Naemura (a1), M. Hashimoto (a1), T. Yamashita (a1), K. Oiwa (a2), J. Seki (a3), H. Suganuma (a4), T. Inakuma (a4) and T. Yoshida (a5)...

The prevention of arterial thrombotic diseases has a high priority in developed countries. An inappropriate diet may be an important risk factor for thrombotic events. The daily intake of an anti-thrombotic diet may offer a convenient and effective way of prevention. The aim of the present study was to test tomato extracts for anti-thrombotic effects and to identify those varieties that have such an effect. A shear-induced platelet-function test (haemostatometry) was used to test anti-thrombotic potential in vitro. Extracts from those tomato varieties that showed a significant anti-thrombotic activity in vitro were further assessed in vivo, using a laser-induced thrombosis test in mice. One tomato variety (KG99-4) showed significant anti-thrombotic activity both in vitro and in vivo. KG99-4 inhibited not only platelet-rich thrombus formation but also had a thrombolytic effect. It is concluded that haemostatometry can detect and classify the anti-thrombotic potential of fruits and vegetables and offers a simple way of screening for such effects.

    • Send article to Kindle

      To send this article to your Kindle, first ensure is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the or variations. ‘’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Tomatoes have natural anti-thrombotic effects
      Available formats
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Tomatoes have natural anti-thrombotic effects
      Available formats
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Tomatoes have natural anti-thrombotic effects
      Available formats
Corresponding author
*Corresponding Author: Dr J. Yamamoto, fax +81 78 974 5689, email
Hide All
Arab, L & Steck, S (2000) Lycopene and cardiovascular diseases. Am J Clin Nutr 71, Suppl.1691S1695S.
Bazzano, LA, He, J, Ogden, LG, et al. (2002) Fruit and vegetable intake and risk of cardiovascular disease in US adults: the first national health and nutrition examination survey epidemiologic follow-up study. Am J Clin Nutr 76, 9399.
Briggs, WH, Folts, JD, Osman, HE & Goldman, IL (2001) Administration of raw onion inhibits platelet-mediated thrombosis in dogs. J Nutr 131, 26192622.
Brown, BG, Zhao, XQ, Chait, A, et al. . (2001) Simvastatin and niacin, antioxidant vitamins, or the combination for the prevention of coronary disease. N Engl J Med 345, 15831592.
Demrow, HS, Slane, PR & Folts, JD (1995) Administration of wine and grape juice inhibits in vivo platelet activity and thrombosis in stenosed canine coronary arteries. Circulation 91, 11821188.
Dutta-Roy, AK, Crosbie, L & Gordon, MJ (2001) Effects of tomato extract on human platelet aggregation in vitro. Platelets 12, 218227.
Fuster, V, Badimon, L, Badimon, JJ & Chesebro, JH, (1992) The pathogenesis of coronary artery disease and the acute coronary syndromes. N Engl J Med 326, 242251.
Gillman, MW, Cupples, LA, Gagnon, D, et al. (1995) Protective effect of fruits and vegetables on development of stroke in men. JAMA 273, 11131117.
Gorog, DA & Kovacs, IB (1995) Thrombotic status analyzer. Measurement of platelet-rich thrombus formation and lysis in native blood. Thromb Haemost 73, 514520.
Gorog, P & Kovacs, IB (1990) Modelling coronary thrombosis from nonanticoagulated human blood in vitro. Hematol Pathol 4, 4352.
Ijiri, Y, Miura, M, Hashimoto, M et al. . (2002) A new model to evaluate the diet-induced prothrombotic state, using He-Ne laser-induced thrombogenesis in the carotid artery of apolipoprotein receptor E-deficient and low-density lipoprotein receptor-deficient mice. Blood Coagul Fibrinolysis 13, 497504.
Joshipura, KJ, Ascheio, A, Manson, JE, et al. (1999) Fruit and vegetable intake in relation to risk of ischemic stroke. JAMA 282, 12331239.
Joshipura, KJ, Hu, FB, Manson, JE, et al. . (2001) The effect of fruit and vegetable intake on risk for coronary heart disease. Ann Intern Med 134, 11061114.
Keevil, JG, Osman, HE, Reed, JD & Folts, JD (2000) Grape juice, but not orange juice or grapefruit juice, inhibits human platelet aggregation. J Nutr 130, 5356.
Kovacs, IB, Hutton, RA & Kernoff, PB (1989) Hemostatic evaluation in bleeding disorders from native blood. Clinical experience with the hemostatometer. Am J Clin Pathol 91, 271279.
Kovacs, IB, Tigyi-Sebes, A, Trombitas, K & Gorog, P (1975) Evans blue: an ideal energy-absorbing material to produce intravascular microinjury by HE-NE gas laser. Microvasc Res 10, 107124.
Liu, S, Manson, JE, Lee, I-M, et al. (2000) Fruit and vegetable intake and risk of cardiovascular disease: the women's health study. Am J Clin Nutr 72, 922928.
Mukamal, KJ, Conigrave, KM, Mittleman, MA, et al. (2003) Roles of drinking pattern and type of alcohol consumption in coronary heart disease in men. N Engl J Med 348, 918.
Nakajima, S, Noguchi, T, Taka, T, et al. (2000) A global platelet test of thrombosis and thrombolysis detects a prothrombotic state in some patients with non-insulin dependent diabetes and in some patients with stroke. Platelets 11, 459466.
Osman, HE, Maalej, N, Shanmuganayagam, D & Folts, JD (1998) Grape juice but not orange or grapefruit juice inhibits platelet activity in dogs and monkeys ( Macaca fasciularis ). J Nutr 128, 23072312.
Ratnatunga, CP, Edmondson, SF, Rees, GM & Kovacs, IB (1992) High-dose aspirin inhibits shear-induced platelet reaction involving thrombin generation. Circulation 85, 10771082.
Renaud, S & de Lorgeril, M (1992) Wine, alcohol, platelets, and the French paradox for coronary heart disease. Lancet 339 15231526.
Shanmuganayagam, D, Beahm, MR, Osman, HE, Krueger, GG, Reed, JD & Folts, JD (2002) Grape seed and grape skin extracts elicit a greater antiplatelet effect when used in combination than when used individually in dogs and humans. J Nutr 132, 35923598.
Sixma, JJ (1994) Interaction of blood platelets with the vessel wall. In Haemostasis and Thrombosis 259285 [Bloom, AL, Forbes, CD, Thomas, DP and Tuddenham, EGD, editors]. London: Churchill Livingstone.
Stein, JH, Keevil, JG, Wiebe, DA, Aeschlimann, S & Folts, JD (1999) Purple grape juice improves endothelial function and reduces the susceptibility of LDL cholesterol to oxidation in patients with coronary artery disease. Circulation 100, 10501055.
Taka, T, Ono, H, Sasaki, Y, Seki, J & Yamamoto, J, (2002) Platelet reactivity in spontaneously diabetic rats is independent from blood glucose and insulin levels. Platelets 13, 313316.
Truelsen, T, Gronbaek, M, Schnohr, P & Boysen, G (1998) Intake of beer, wine, and spirits and risk of stroke: the Copenhagen city heart study. Stroke 29, 24672472.
Ulbricht, TL & Southgate, DA (1991) Coronary heart disease: seven dietary factors. Lancet 338, 985992.
Yamamoto, J, Iizumi, H, Hirota, R, et al. . (1989) Effect of physical training on thrombotic tendency in rats: decrease in thrombotic tendency measured by the He-Ne laser-induced thrombus formation method. Haemostasis 19, 260265.
Yamamoto, J, Ishii, I, Okita, N, et al. . (1997) The differential involvement of von Willebrand factor, fibrinogen and fibronectin in acute experimental thrombosis in rat cerebral and mesenteric microvessels. Jpn J Physiol 47, 431441.
Yamamoto, J, Yamashita, T, Ikarugi, H, et al. (2003) Gorog thrombosis test: a global in vitro test of platelet function and thrombolysis. Blood Coagul Fibrinolysis 14, 3139.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *



Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed