Skip to main content Accessibility help
×
Home

Effect of 4-coumaric and 3,4-dihydroxybenzoic acid on oxidative DNA damage in rat colonic mucosa

  • Francesco Guglielmi (a1), Cristina Luceri (a1), Lisa Giovannelli (a1), Piero Dolara (a1) and Maura Lodovici (a1)...

Abstract

The effect of 4-coumaric and 3,4-dihydroxybenzoic (protocatechuic) acid on the basal oxidative DNA damage of rat colonic mucosa in vivo was studied, relative to vitamin E. F344 rats were treated with 4-coumaric or protocatechuic acid mixed in the diet (25 or 50 mg/kg for 2 weeks). It was observed that 4-coumaric acid (50 mg/kg) significantly decreased the basal level of the oxidative damage assessed as 8-OH-2′-deoxyguanosine levels in DNA and by the comet assay. Moreover, it was found that vitamin E (10 mg/kg) had no effect on colonic mucosa oxidation damage, whereas at a higher dose (55 mg/kg) it actually enhanced oxidative stress. The effect of 4-coumaric acid (50 mg/kg) on the expression of some glutathione-related enzymes (glutathione-S-transferase (GST)-P, GST-M2, GST-M1, γ-glutamylcysteine synthetase, glutathione peroxidase (GSPX)1 and GSPX4) was also investigated at the level of the colonic mucosa. Only the expression of GST-M2 was significantly induced by 4-coumaric acid, while protocatechuic acid was inactive. The data suggest that 4-coumaric acid acts as an antioxidant in the colonic mucosa in vivo.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Effect of 4-coumaric and 3,4-dihydroxybenzoic acid on oxidative DNA damage in rat colonic mucosa
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Effect of 4-coumaric and 3,4-dihydroxybenzoic acid on oxidative DNA damage in rat colonic mucosa
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Effect of 4-coumaric and 3,4-dihydroxybenzoic acid on oxidative DNA damage in rat colonic mucosa
      Available formats
      ×

Copyright

Corresponding author

*Corresponding Author: Dr Francesco Guglielmi, fax +39 055 4271280, email francesco.guglielmi@unifi.it

References

Hide All
Baez, S, Segura-Aguilar, J, Widersten, M, Johasson, AS & Mannervik, B (1997) Glutathione transferases catalyse the detoxication of oxidized metabolites (o-quinones) of catecholamines and may serve as an antioxidant system preventing degenerative cellular process. Biochemical Journal 324 2528.
Brown, JP (1980) A review of the genetic effects of naturally occurring flavonoids, anthraquinones and related compounds. Mutation Research 75 243277.
Burkitt, MJ & Milne, L (1996) Hydroxyl radical formation from Cu(II)-trolox mixture insights into the pro-oxidant properties of α-tocopherol. FEBS Letters 379 5154.
Caderni, G, De Filippo, C, Luceri, C, Salvadori, M, Giannini, A, Biggeri, A, Remy, S, Cheynier, V & Dolara, P (2000) Effects of black tea, green tea and wine extracts on intestinal carcinogenesis induced by azoxymethane (AOM) in F344 rats. Carcinogenesis 21 19651969.
Chou, FP, Chu, YC, Hsu, JD, Chiang, HC & Wang, CJ (2000) Specific induction of glutathione S-transferase GSTM2 subunit expression by epigallocatechin gallate in rat liver. Biochemical Pharmacology 6 643650.
Clifford, MN (2000) Chlorogenic acids and other cinnamates – nature, occurrence, dietary burden, absorption and metabolism. Journal of Science and Food Agriculture 80 10331043.
Eaton, DL & Bammler, TK (1999) Concise review of the glutathione S-Transferases and their significance to Toxicology. Toxicological Science 49 156164.
European Community (1986) European Community Regulations on the Care and Use of Laboratory Animals, Law 86/609/EC.
European Standards Committee on Oxidative DNA Damage (2002) Comparative analysis of baseline 8-oxo-7,8-dihydroguanine in mammalian cell DNA, by different methods in different laboratories: an approach to consensus. Carcinogenesis 23 21292133.
Fahelbum, IM & James, SP (1977) The absorption and metabolism of methyl cinnamate. Toxicology 7 123132.
Fiander, H & Schneider, H (2000) Dietary ortho phenols that induce glutathione S-transferase and increase the resistance of cells to hydrogen peroxide are potential cancer chemopreventives that act by two mechanisms: the alleviation of oxidative stress and the detoxification of mutagenic xenobiotics. Cancer Letters 156 117124.
Giovannelli, L, Testa, G, De Filippo, C, Cheynier, V, Clifford, MN & Dolara, P (2000) Effect of complex polyphenols and tannins from red wine on DNA oxidative damage of rat colon mucosa in vivo. European Journal of Nutrition 39 207212.
Havsteen, B (1983) Flavonoids, a class of natural products of high pharmacological potency. Biochemical Pharmacology 32 11411149.
Hertog, MGL (1995) Flavonoid intake and long term risk of coronary heart disease and cancer in the 7 countries study. Archives of Internal Medicine 155 11841195.
Hertog, MGL, Fesken, EJM, Hollman, PCH, Katan, MB & Kromhout, D (1993) Dietary antioxidant flavonoids and risk of coronary artery disease, the Zutphen Elderly Study. Lancet 342 10071011.
Jacobs, A, White, GP & Tait, GP (1977) Iron chelation in cell cultures by two conjugates of 2,3-dihydroxybenzoic acid (2,3-DHB). Biochemical and Biophysical Research Communications 74 16261630.
Knekt, P, Aromaa, A, Maatela, J, Aaran, RK, Nikkau, T, Hakama, M, Hakulinen, T, Peto, R & Teppo, L (1991) Vitamin E and cancer prevention. American Journal of Clinical Nutrition 53 283286.
Konopacka, M, Widel, M & Rzeszowska-Wolny, J (1998) Modifying effect of Vitamin C, E and beta-carotene against gamma-ray induced DNA damage in mouse cells. Mutation Research 417 8594.
Laranjinha, JA, Almeida, LM & Madeira, VM (1994) Reactivity of dietary phenolic acids with peroxyl radicals: antioxidant activity upon low density lipoprotein peroxidation. Biochemical Pharmacology 48 487494.
Lii, CK, Sung, WC, Ko, YJ & Chen, HW (1998) Alpha-Tocopherol acetate supplementation enhances rat hepatic cytochrome PROD activity in the presence of Phenobarbital induction. Nutrition and Cancer 32 3742.
Lodovici, M, Casalini, C, De Filippo, C, Copeland, E, Xu, X, Clifford, M & Dolara, P (2000) Inhibition of 1,2-dimethylhydrazine-induced oxidative DNA damage in rat colon mucosa by black tea complex polyphenols. Food and Chemical Toxicology 38 10851088.
Lodovici, M, Guglielmi, F, Meoni, M & Dolara, P (2001) Effect of natural phenolic acids on DNA oxidation in vitro. Food and Chemical Toxicology 39 12051210.
Masella, R, Cantafora, A, Modesti, D, Cardilli, A, Gennaro, L, Bocca, A & Coni, E (1999) Antioxidant activity of 3,4-DHPEA-EA and protocatechuic acid: a comparative assessment with other olive oil biophenols. Redox Reports 4 113121.
Mitchel, REJ & McCann, R (1993) Vitamin E is a complete tumour promoter in mouse skin. Carcinogenesis 14 659662.
Morton, LW, Croft, KD, Puddey, IB & Byrne, L (2000) Phenolic acids protect low density lipoproteins from peroxynitrite-mediated modification in vitro. Redox Reports 5 124125.
Owen, RW, Giacosa, A, Hull, WE, Haubner, R, Spiegelhalder, B & Bartsch, H (2000) The antioxidant/anticancer potential of phenolic compounds isolated from olive oil. European Journal of Cancer 36 12351247.
Ramirez-Tortosa, C, Andersen, OM, Gardner, PT, Morrice, PC, Wood, SG, Duthie, SJ, Collins, AR & Duthie, GG (2001) Anthocyanin-rich extract decreases indices of lipid peroxidation and DNA damage in vitamin E-depleted rats. Free Radical Biology and Medicine 1 10331037.
Rimm, EB, Ascherio, A, Giovannucci, E, Spiegelman, D, Stampfer, MJ & Willett, WC (1996) Vegetable, fruit, and cereal fiber intake and risk of coronary heart disease among men. Journal of the American Medical Association 275 447451.
Rimm, EB, Stampfer, MJ, Ascherio, A, Giovannucci, E, Colditz, GA & Willet, WC (1993) Vitamin E consumption and the risk of coronary heart disease in men. New England Journal of Medicine 328 14501456.
Satué-Gracia, MT, Andrés-Lacueva, C, Lamuela-Raventós, RM & Frankel, EN (1999) Inhibitors of in Vitro Human Low-Density Lipoprotein Oxidation. Journal of Agriculture and Food Chemistry 47 21982202.
Steel, VE, Kelloff, GJ, Balentine, D, Boone, CW, Mehta, R, Bagheri, D, Sigman, CC, Zhu, S & Sharma, SVE (2000) Comparative chemopreventive mechanisms of green tea, black tea and selected polyphenol extracts measured by in vitro bioassays. Carcinogenesis 21 6367.
Stich, HF (1991) The beneficial and hazardous effects of simple phenolic compounds. Mutation Research 259 307324.
Stich, HF, Ohshima, H, Pignatelli, B, Michelon, J & Bartsch, H (1983) Inhibitory effect of betel nut extracts on endogenous nitrosation in humans. Journal of the National Cancer Institute 70 10471050.
Tomàs-Barberan, FA & Clifford, MN (2000) Dietary hydroxibenzoic acid derivatives – nature, occurrence and dietary burden. Journal of Science and Food Agriculture 80 10241032.
Tseng, TH, Wang, CJ, Kao, ES & Chu, HY (1996) Hibiscus protocatechuic acid protects against oxidative damage induced by tert-butylhydroperoxide in rat primary hepatocytes. Chemical Biological Interaction 101 137148.
Ueda, J, Saito, N, Shimazu, Y & Ozawa, TA (1996) Comparison of scavenging abilities of antioxidant against hydroxyl radicals. Archives of Biochemistry and Biophysics 333 377383.
Willet, WC (2000) Diet and cancer. Oncologist 5 393404.

Keywords

Effect of 4-coumaric and 3,4-dihydroxybenzoic acid on oxidative DNA damage in rat colonic mucosa

  • Francesco Guglielmi (a1), Cristina Luceri (a1), Lisa Giovannelli (a1), Piero Dolara (a1) and Maura Lodovici (a1)...

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed