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Effect of copper supplementation on indices of copper status and certain CVD risk markers in young healthy women

  • Susanne Bügel (a1), Angela Harper (a1), Edmond Rock (a2), Jacqueline M. O'Connor (a3), Maxine P. Bonham (a3) and J. J. Strain (a3)...

Abstract

Western diets containing suboptimal Cu concentrations could be widespread. A link between marginal Cu deficiency and CVD has been suggested. The objective of the present study was to investigate the effect of Cu supplementation on both Cu status and CVD risk factors in healthy young women. Sixteen women with a mean age of 24 (sd 2) years participated in a randomised crossover study of three 4-week periods with 3-week washouts between periods. During each intervention period, subjects received 0, 3 or 6 mg elemental Cu/d as CuSO4 in addition to their habitual diet. Blood samples were taken to assess the effect of supplementation on putative markers of Cu status. The content of plasma lipids, lipoprotein (a), apo and certain haemostatic factors, as putative indices of CVD, was also analysed. Daily supplementation with 3 mg Cu significantly increased (P<0·05) serum Cu concentration and the activity of erythrocyte superoxide dismutase, although there was no further significant increase after an intake of 6 mg Cu/d. The concentration of the fibrinolytic factor plasminogen activator inhibitor type 1 was significantly reduced (P<0·05) by about 30% after supplementation with 6 mg Cu/d. No other marker of Cu status or CVD risk factor was affected by Cu supplementation. The results indicate that supplementation with 3 or 6 mg Cu/d may improve Cu status in these healthy young women. Increased Cu intake could reduce the risk of CVD and atherosclerosis in man by promoting improved fibrinolytic capacity.

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Copyright

Corresponding author

*Corresponding author: Dr Susanne Bügel, fax +45 35 28 24 83, email shb@kvl.dk

References

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Allen, KGD & Klevay, LM (1978) Cholesterolemia and cardiovascular abnormalities in rats caused by Cu deficiency. Atherosclerosis 29, 8193.
Aznar, J & Estelle, A (1994) Role of plasminogen activator inhibitor type 1 in the pathogenesis of coronary heart diseases. Haemostasis 24, 243251.
Baker, A, Turley, E, Bonham, MO, O'Connor, JM, Strain, JJ, Flynn, A & Cashman, KD (1999) No effect of copper supplementation on biochemical markers of bone metabolism in healthy adults. Br J Nutr 82, 283290.
Bro, S, Sandström, B & Heydorn, K (1990) Intake of essential and toxic trace elements in a random sample of Danish men as determined by the duplicate portion sampling technique. J Trace Elem Electrolytes Health Dis 4, 147155.
Clauss, A (1957) Rapid physiological coagulation method for the determination of fibrinogen [German]. Acta Haematol 17, 237246.
Danks, DM (1988) Copper deficiency in humans. Annu Rev Nutr 8, 235257.
Food and Nutrition Board (2001) Copper. In Dietary Reference Intakes for Vitamin A, Vitamin K, Arsenic, Boron, Chromium, Copper, Iodine, Iron, Manganese, Molybdenum, Nickel, Silicon, Vanadium, and Zinc, pp. 224257Washington, DC: National Academy Press.
Harvey, LJ, Majsak-Newman, G, Dainty, JR, Lewis, DJ, Langford, NJ, Crews, HM & Fairweather-Tait, SJ (2003) Adaptive responses in men fed low- and high-copper diets. Br J Nutr 90, 161168.
Johnson, PE, Milne, DB & Lykken, GI (1992) Effects of age and sex on copper absorption, biological half- life, and status in humans. Am J Clin Nutr 56, 917925.
Johnson, PE, Stuart, MA, Hunt, JR, Mullen, L & Starks, TL (1988) Copper absorption by women fed intrinsically and extrinsically labeled goose meat, goose liver, peanut butter and sunflower butter. J Nutr 118, 15221528.
Jones, AA, DiSilvestro, RA, Coleman, M & Wagner, TL (1997) Copper supplementation of adult men: effects on blood copper enzyme activities and indicators of cardiovascular disease risk. Metab Clin Exp 46, 13801383.
Jones, DG & Suttle, NF (1981) Some effects of Cu deficiency on leucocyte function in cattle and sheep. Res Vet Sci 31, 151156.
Kehoe, CA, Turley, E, Bonham, MP, et al. (2000) Response of putative indices of copper status to copper supplementation in human subjects. Br J Nutr 84, 151156.
Klevay, LM (1975) Coronary heart disease: the zinc/Cu hypothesis. Am J Clin Nutr 28, 764774.
Klevay, LM (1998) Lack of a recommended dietary allowance for copper may be hazardous to your health. J Am Coll Nutr 17, 322326.
Klevay, LM, Buchet, JP, Bunker, VW, et al. (1993) Copper in the Western diet. In Trace Elements in Man and Animals, pp. 207210 [Anke, M, Meissner, D and Mills, CF, editors]. Gersdorf, Germany: Verlag Media Touristik.
Klevay, LM, Inman, L, Johnson, LK, Lawler, M, Mahalko, JR, Milne, DB, Lukaski, HC, Bolonchuk, W & Sandstead, HH (1984) Increased cholesterol in plasma in a young man during experimental copper depletion. Metabolism 33, 11121118.
Lau, BW & Klevay, LM (1981) Plasma lecithin:cholesterol acyltransferase in copper deficient rats. J Nutr 111, 16981703.
Lynch, SM & Klevay, LM (1993) Effect of a dietary copper deficiency on plasma fibrinolytic activity in male and female mice. Nutr Res 13, 913922.
Medeiros, DM, Liao, Z & Hamlin, RL (1991 a) Copper deficiency in a genetically hypersensitive cardiomyopathic rat: 3 lectrocardiogram, functional and ultrastructural aspects. J Nutr 121, 10261034.
Medeiros, DM, Milton, A, Brunette, E & Stacy, L (1991 b) Copper supplementation effects on indicators of copper status and serum-cholesterol in adult males. Biol Trace Elem Res. 30, 1935.
Milne, DB (1998) Copper intake and assessment of copper status. Am J Clin Nutr 67, Suppl, 1041S1045S.
Milne, DB, Johnson, PE, Klevay, LM & Sandstead, HH (1990) Effect of copper intake on balance, absorption, and status indices of copper in men. Nutr Res 10, 975986.
Milne, DB, Klevay, LM & Hunt, JR (1988) Effects of ascorbic acid supplements and a diet marginal in copper on indices of copper nutriture in women. Nutr Res 8, 865873.
Milne, DB & Nielsen, FH (1996) Effects of a diet low in copper on copper-status indicators in postmenopausal women. Am J Clin Nutr 63, 358364.
National Research Council (1989) Recommended Dietary Allowances, 10th ed. Washington, DC: National Academy Press.
Nielsen, FH, Milne, DB, Mullen, LM & Gallagher, SK (1990) Dietary sulfur amino acids and genetic make-up or interindividual variation affect the response of men to Cu depletion. J Trace Elem Exp Med 3, 281296.
Pang, Y, MacIntosh, DL & Ryan, PB (2001) A longitudinal investigation of aggregate oral intake of copper. J Nutr 131, 21712176.
Rayssiguier, Y, Gueux, E, Bussiere, L & Mazur, A (1993) Copper deficiency increases the susceptibility of lipoproteins and tissues to peroxidation in rats. J Nutr 123, 13431348.
Reiser, S, Powell, A, Yang, C-Y & Canary, JJ (1987) Effect of copper intake on blood cholesterol and its lipoprotein distribution in men. Nutr Rep Int 36, 641649.
Reiser, S, Smith, JC Jr, Mertz, W, Holbrook, JT, Schofield, DJ, Powell, AS, Canfield, WK & Canary, JJ (1985) Indices of copper status in humans consuming a typical American diet containing either fructose or starch. Am J Clin Nutr 42, 242251.
Rock, E, Mazur, A, O'Connor, JM, Bonham, MP, Rayssiguier, Y & Strain, JJ (2000) The effect of copper supplementation on red blood cell oxidizability and plasma antioxidants in middle-aged healthy volunteers. Free Radic Biol Med 28, 324329.
Salmenpera, L, Siimes, MA, Nanto, V & Perheentupa, J (1989) Copper supplementation: failure to increase plasma copper and ceruloplasmin concentrations in healthy infants. Am J Clin Nutr 50, 843847.
Strain, JJ (1994) Newer aspects of micronutrients in chronic disease: copper. Proc Nutr Soc 53, 583598.
Turnlund, JR, Jacob, RA, Keen, CL, Strain, JJ, Kelley, DS, Domek, JM, Keyes, WR, Ensunsa, JL, Lykkesfeldt, J & Coulter, J (2004) Long-term high copper intake: effects on indexes of copper status, antioxidant status, and immune function in young men. Am J Clin Nutr 79, 10371044.
Turnlund, JR, Keen, CL & Smith, RG (1990) Copper status and urinary and salivary copper in young men at three levels of dietary copper. Am J Clin Nutr 51, 658664.
Viestenz, KE & Klevay, LM (1982) A randomized trial of copper therapy in rats with electrocardiographic abnormalities due to copper deficiency. Am J Clin Nutr 35, 258266.
Walker, FJ & Fay, PJ (1990) Characterization of an interaction between protein C and ceruloplasmin. J Biol Chem 265, 18341836.

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