Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
Home
Hostname: page-component-ffbbcc459-p4gdp Total loading time: 0.739 Render date: 2022-03-14T19:56:31.311Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "useRatesEcommerce": false, "useNewApi": true }
  • English
British Journal of Nutrition British Journal of Nutrition

Article contents

Effect of phylloquinone supplementation on biochemical markers of vitamin K status and bone turnover in postmenopausal women

Published online by Cambridge University Press:  01 February 2007

Susanne Bügel ,
A. Dorthe Sørensen ,
Ole Hels ,
Mette Kristensen ,
Cees Vermeer ,
Jette Jakobsen ,
Albert Flynn ,
Christian Mølgaard  and
Kevin D. Cashman
Susanne Bügel*
Affiliation:
Department of Human Nutrition/Centre for Advanced Food Studies, The Royal Veterinary and Agricultural University, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark
A. Dorthe Sørensen
Affiliation:
Department of Human Nutrition/Centre for Advanced Food Studies, The Royal Veterinary and Agricultural University, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark
Ole Hels
Affiliation:
Department of Human Nutrition/Centre for Advanced Food Studies, The Royal Veterinary and Agricultural University, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark
Mette Kristensen
Affiliation:
Department of Human Nutrition/Centre for Advanced Food Studies, The Royal Veterinary and Agricultural University, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark
Cees Vermeer
Affiliation:
Department of Biochemistry, University of Maastricht, Maastricht, The Netherlands
Jette Jakobsen
Affiliation:
Danish Institute for Food and Veterinary Research, Søborg, Denmark
Albert Flynn
Affiliation:
Department of Food and Nutritional Sciences, University College Cork, Cork, Republic of Ireland
Christian Mølgaard
Affiliation:
Department of Human Nutrition/Centre for Advanced Food Studies, The Royal Veterinary and Agricultural University, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark
Kevin D. Cashman
Affiliation:
Department of Food and Nutritional Sciences, University College Cork, Cork, Republic of Ireland
*
*Associate Professor Susanne Bügel, fax +45 35282483, shb@kvl.dk
Rights & Permissions[Opens in a new window]

Abstract

HTML view is not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

While current intakes of phylloquinone (vitamin K1) in many populations are believed to be sufficient to maintain normal blood coagulation, these may be insufficient to cover the requirements for optimal bone metabolism. Therefore, the objective of the present study was to investigate the effect of increasing phylloquinone intakes above the usual dietary intake for 6 weeks on biochemical markers of vitamin K status and bone turnover in postmenopausal women. Thirty-one postmenopausal women completed this 3 × 6-week randomised cross-over study, in which volunteers were supplemented with 0 (placebo), 200, and 500 μg phylloquinone/d. In addition, the volunteers were given 10 μg vitamin D3/d throughout the study period. With increasing phylloquinone intake, the concentration of serum γ-carboxylated and under-γ-carboxylated osteocalcin was significantly increased and decreased, respectively, in a dose-dependent manner (P < 0·001). Mean serum phylloquinone concentration was significantly (P < 0·001) higher with daily supplementation with 500 μg phylloquinone/d compared with that during either of the placebo or 200 μg phylloquinone/d supplementation periods, which did not differ (P = 0·15). Serum total osteocalcin was significantly (P < 0·001) increased in response to daily supplementation with 500 (but not 200) μg phylloquinone compared with placebo. Serum bone-specific alkaline phosphatase as well as the urinary markers of bone resorption (N-telopeptide cross-links of collagen, pyridinoline and deoxypyridinoline) and urinary γ-carboxyglutamate were unaffected by phylloquinone supplementation. In conclusion, while daily supplementation with 200 and 500 μg phylloquinone/d for 6 weeks increased vitamin K status in postmenopausal women, it had no effect on bone turnover.

Keywords

Bone biochemical markersPhylloquinone supplementationVitamin K statusPostmenopausal womenOsteocalcin
Type
Research Article
Information
British Journal of Nutrition , Volume 97 , Issue 2 , February 2007 , pp. 373 - 380
Copyright
Copyright © The Authors 2007

References

Becker, W, Lyhne, N, Pedersen, AN, Aro, A, Fogelhol, M, Pedersen, JI, Alexander, J, Andersen, SA, Meltzer, HM & Pórsdóttir, I (2004) Nordic Nutrition Recommendations 2004 – Integrating Nutrition and Physical Activity, pp. 313–322. Copenhagen: Nord, Nordic Council of Ministers.Google Scholar
Bingham, S & Cummings, JH (1983) The use of 4-aminobenzoic acid as a marker to validate the completeness of 24 hr urine collection in man. Clin Sci 64, 629–635.CrossRefGoogle Scholar
Binkley, NC, Krueger, DC, Engelke, JA, Foley, AL & Suttie, JW (2000) Vitamin K supplementation reduces serum concentrations of under-γ-carboxylated osteocalcin in healthy young and elderly adults. Am J Clin Nutr 72, 1523–1528.CrossRefGoogle Scholar
Binkley, NC, Krueger, DC, Kawahara, TN, Engelke, JA, Chappell, RJ & Suttie, JW (2002) A high phylloquinone intake is required to achieve maximal osteocalcin γ-carboxylation. Am J Clin Nutr 76, 1055–1060.CrossRefGoogle ScholarPubMed
Binkley, NC & Suttie, JW (1995) Vitamin K nutrition and osteoporosis. J Nutr 125, 1812–1821.CrossRefGoogle ScholarPubMed
Booth, SL, Broe, KE, Gagnon, DR, Tucker, KL, Hannan, MT, McLean, RR, Dawson-Hughes, B, Wilson, PWF, Cupples, A & Kiel, DP (2003) Vitamin K intake and bone mineral density in women and men. Am J Clin Nutr 77, 512–516.CrossRefGoogle ScholarPubMed
Booth, SL, Lichtenstein, AH, O'Brien-Morse, M, McKeown, NM, Wood, RJ, Saltzman, E & Gundberg, CM (2001) Effects of a hydrogenated form of vitamin K on bone formation and resorption. Am J Clin Nutr 74, 783–790.CrossRefGoogle ScholarPubMed
Booth, SL, Tucker, KL, Chen, H, et al. (2000) Dietary vitamin K intakes are associated with hip fracture but not with bone mineral density in elderly men and women. Am J Clin Nutr 71, 1201–1208.CrossRefGoogle Scholar
Booth, SL, Webb, R & Peters, JC (1999) Assessment of phylloquinone and dihydrophylloquinone dietary intakes among a nationally representative sample of US consumers using 14-day food diaries. J Am Diet Assoc 99, 1072–1076.CrossRefGoogle ScholarPubMed
Braam, LA, Knapen, MH, Geusens, P, Brouns, F, Hamulyak, K, Gerichhausen, MJ & Vermeer, C (2003) Vitamin K1 supplementation retards bone loss in postmenopausal women between 50 and 60 years of age. Calcif Tissue Int 73, 21–26.CrossRefGoogle ScholarPubMed
Bügel, S (2003) Vitamin K and bone health. Proc Nutr Soc 62, 839–843.CrossRefGoogle ScholarPubMed
Calabresi, E, Lasagni, L, Franceschelli, F, Bartolini, L, Serio, M & Brandi, ML (1994) Use of an internal standard to measure pyridinoline and deoxypyridinoline in urine (letter). Clin Chem 40, 336–337.Google Scholar
Colwell, A, Russel, RG & Eastell, R (1993) Factors affecting the assay of urinary 3-hydroxypyridinium crosslinks of collagen as markers of bone resorption. Eur J Clin Invest 23, 341–349.CrossRefGoogle Scholar
Department of Health (1991) Calcium. In Dietary Reference Values for Food Energy and Nutrients for the United Kingdom. Report in Health and Social Subjects no. 41, pp. 150–157. London, UK: H.M. Stationery Office.Google Scholar
Douglas, AS, Robins, SP, Hutchison, JD, Porter, RW, Stewart, A & Reid, DM (1995) Carboxylation of osteocalcin in post-menopausal osteoporotic women following vitamin K and D supplementation. Bone 17, 15–20.CrossRefGoogle ScholarPubMed
Duggan, P, Cashman, KD, Flynn, A, Bolton-Smith, C & Kiely, M (2004) Phylloquinone (vitamin K1) intakes and food sources in 18–64-year-old Irish adults. Br J Nutr 92, 151–158.CrossRefGoogle ScholarPubMed
Feskanich, D, Weber, P, Willett, WC, Rockett, H, Booth, SL & Colditz, GA (1999) Vitamin K intake and hip fractures in women: a prospective study. Am J Clin Nutr 69, 74–79.CrossRefGoogle ScholarPubMed
Gijsbers, BL, Jie, KSG & Vermeer, C (1996) Effect of food composition on vitamin K absorption in human volunteers. Br J Nutr 76, 223–229.CrossRefGoogle ScholarPubMed
Gundberg, CM, Nieman, SD, Abrams, S & Rosen, H (1998) Vitamin K status and bone health: an analysis of methods for determination of undercarboxylated osteocalcin. J Clin Endocrinol Metab 83, 3258–3266.Google ScholarPubMed
Institute of Medicine (1997) Dietary Reference Intakes for Calcium, Phosphorus, Magnesium, Vitamin D, and Fluoride, pp. 71–145. Washington, DC: National Academies Press.Google Scholar
Institute of Medicine (2001) Dietary Reference Intakes for Vitamin A, Vitamin K, Arsenic, Boron, Chromium, Copper, Iodine, Iron, Manganese, Molybdenum, Nickel, Silicon, Vanadium and Zinc, pp. 162–196. Washington, DC: National Academies Press.Google Scholar
Knapen, MHJ, Hamulyák, K & Vermeer, C (1989) The effect of vitamin K supplementation on circulating osteocalcin (bone Gla protein) and urinary calcium excretion. Ann Intern Med 111, 1001–1005.CrossRefGoogle ScholarPubMed
Knapen, MHJ, Jie, KSG, Hamulyák, K & Vermeer, C (1993) Vitamin K-induced changes in markers for osteoblast activity and urinary calcium loss. Calcif Tissue Int 53, 81–85.CrossRefGoogle ScholarPubMed
Kuwada, M & Katayama, K (1983) An improved method for determination of gamma-carboxyglutamic acid in proteins, bone and urine. Anal Biochem 131, 173–179.CrossRefGoogle ScholarPubMed
McKeown, NM, Jacques, PF, Gundberg, CM, Peterson, JW, Tucker, KL, Kiel, DP, Wilson, PWF & Booth, SL (2002) Dietary and nondietary determinants of vitamin K biochemical measures in men and women. J Nutr 132, 1329–1334.CrossRefGoogle ScholarPubMed
Mølgaard, C, Sandström, B & Michaelsen, KF (1998) Evaluation of a food frequency questionnaire for assessing of calcium, protein and phosphorus intakes in children and adolescents. Scand J Nutr 42, 2–5.Google Scholar
Nishimoto, SK & Price, PA (1985) The vitamin K-dependent bone protein is accumulated within cultured osteosarcoma cells in the presence of the vitamin K antagonist warfarin. J Biol Chem 260, 2832–2836.Google ScholarPubMed
Plantalech, L, Chapuy, MC, Guillaumont, M, Chapuy, P, Leclerq, M & Delmas, DP (1990) Impaired carboxylation of serum osteocalcin in elderly women: effect of vitamin K1 treatment. In Osteoporosis, pp. 345–347 [Christiansen, C and Overgaard, K, editors]. Copenhagen: Osteopress.Google Scholar
Robins, SP, Stead, DA & Duncan, A (1994) Precautions in using an internal standard to measure pyridinoline and deoxypyrodinoline in urine (letter). Clin Chem 40, 2322–2323.Google Scholar
Ronden, JE, Thijssen, HH & Vermeer, C (1998) Tissue distribution of K-vitamers under different nutritional regimens in the rat. Biochim Biophys Acta 1379, 16–22.CrossRefGoogle ScholarPubMed
Sadowski, JA, Hood, SJ, Dallal, GE & Garry, PJ (1989) Phylloquinone in plasma from elderly and young adults: factors influencing its concentration. Am J Clin Nutr 50, 100–108.CrossRefGoogle Scholar
Schurgers, LJ, Geleijnse, JM, Grobbee, DE, Pols, HAP, Hofman, A, Wittemann, JCM & Vermeer, C (1999) Nutritional intake of vitamins K1 (phylloquinone) and K2 (menaquinone) in the Netherlands. J Nutr Environm Med 9, 115–122.CrossRefGoogle Scholar
Shearer, MJ (1997) The roles of vitamin D and K in bone health and osteoporosis prevention. Proc Nutr Soc 56, 915–937.CrossRefGoogle Scholar
Shearer, MJ (2000) Role of vitamin K and Gla proteins in the pathophysiology of osteoporosis and vascular calcification. Curr Opin Clin Nutr Metab Care 3, 433–438.CrossRefGoogle ScholarPubMed
Sokoll, LJ & Sadowski, JA (1996) Comparison of biochemical indexes for assessing vitamin K nutritional status in a healthy adult population. Am J Clin Nutr 63, 566–573.CrossRefGoogle Scholar
Szulc, P, Arlot, M, Chapuy, MC, Duboeuf, F, Muenier, PJ & Delmas, PD (1994) Serum undercarboxylated osteocalcin correlates with hip bone mineral density in elderly women. J Bone Miner Res 9, 1591–1595.CrossRefGoogle ScholarPubMed
Szulc, P, Chapuy, MC, Meunier, PJ & Delmas, PD (1993) Serum undercarboxylated osteocalcin is a marker of the risk of hip fracture in elderly women. J Clin Invest 91, 1769–1774.CrossRefGoogle ScholarPubMed
Szulc, P, Chapuy, MC, Meunier, PJ & Delmas, PD (1996) Serum undercarboxylated osteocalcin is a marker of the risk of hip fracture: a three year follow-up study. Bone 18, 487–488.CrossRefGoogle ScholarPubMed
Thane, CW, Paul, AA, Bates, CJ, Bolton-Smith, C, Prentice, A & Shearer, MJ (2002) Intake and sources of phylloquinone (vitamin K1): variation with socio-demographic and lifestyle factors in a national sample of British elderly people. Br J Nutr 87, 605–613.CrossRefGoogle Scholar
Vergnaud, P, Garnero, P, Meunier, PJ, Breart, G, Kamihagi, K & Delmas, PD (1997) Undercarboxylated osteocalcin measured with a specific immunoassay predicts hip fracture in elderly women: the EPIDOS study. J Clin Endocrinol Metab 82, 719–724.Google ScholarPubMed
Vermeer, C & Braam, L (2001) Role of K vitamins in the regulation of tissue calcification. J Bone Miner Metab 19, 201–206.CrossRefGoogle ScholarPubMed
Vermeer, C, Gijsbers, BLM, Craciun, AM, Groenen-Van Dooren, MM & Knapen, MH (1996) Effects of vitamin K on bone mass and bone metabolism. J Nutr 126, 1187S–1191S.CrossRefGoogle Scholar
Vermeer, C, Shearer, MJ, Zittermann, A, Bolton-Smith, C, Szulc, P, Hodges, S, Walter, P, Rambeck, W, Stöcklin, E & Weber, P (2004) Beyond deficiency: potential benefits of increased intakes of vitamin K for bone and vascular health. Eur J Nutr 5, 1–11.Google Scholar
Weber, P (2001) Vitamin K and bone health. Nutr 17, 880–887.CrossRefGoogle ScholarPubMed
World Health Organization (1994) Assessment of Fracture Risk and its Application to Screening for Postmenopausal Osteoporosis. Technical Report Series no. 843. Geneva: WHO.Google Scholar
Wu, W, Bancroft, JD & Suttie, JW (1996) Differential effects of warfarin on the intracellular processing of vitamin K-dependent proteins. Thromb Haemost 76, 46–52.Google ScholarPubMed
You have Access
29
Cited by

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Effect of phylloquinone supplementation on biochemical markers of vitamin K status and bone turnover in postmenopausal women
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Effect of phylloquinone supplementation on biochemical markers of vitamin K status and bone turnover in postmenopausal women
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Effect of phylloquinone supplementation on biochemical markers of vitamin K status and bone turnover in postmenopausal women
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *