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Effect of reduced maternal protein intake in pregnancy in the rat on the fatty acid composition of brain, liver, plasma, heart and lung phospholipids of the offspring after weaning

  • Graham C. Burdge (a1), Emmanuelle Delange (a1), Ludivine Dubois (a1), Rebecca L. Dunn (a1), Mark A. Hanson (a2), Alan A. Jackson (a1) and Philip C. Calder (a1)
  • DOI:
  • Published online: 01 March 2007

Reduced protein intake during pregnancy decreased maternal hepatic and plasma docosahexaenoic acid concentrations and impaired docosahexaenoic acid accumulation into fetal brain in the rat. The present study investigated whether restriction of maternal protein intake during pregnancy in the rat alters membrane phospholipid fatty acid composition in the offspring after weaning. Female rats (six per group) were mated and fed diets containing either 180 or 90 g protein/kg throughout pregnancy. Mothers were transferred to standard chow after delivery and the litters reduced to eight pups. Weaning was at 28 d and pups were killed 5 to 6 d later. Tissue weights or membrane total phosphatidylcholine (PC) and phosphatidylethanolamine (PE) concentrations in the offspring did not differ between dietary groups. There were significant differences between the 180 and 90 g/kg groups in liver, brain, lung and heart fatty acid composition that differed between tissues and phospholipid classes. For example, docosahexaenoic and arachidonic acid concentrations were 23 and 10 % lower respectively in hepatic PC, but not PE, in the 90 g/kg group. In brain, docosahexaenoic acid concentration was 17 % lower in PC, but not PE, while arachidonic acid content was 21 % greater in PE but unchanged in PC. The greatest differences were in unsaturated fatty acids, which suggests alterations to desaturase activities and/or the specificity of phospholipid biosynthesis. These results suggest that restricted maternal protein intake during pregnancy results in persistent alterations to membrane fatty acid content.

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*Corresponding author: Dr G. C. Burdge, fax +44 23 80594383, email
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GC Burdge , AN Hunt & AD Postle (1994) Mechanisms of hepatic phosphatidylcholine synthesis in adult rat: effects of pregnancy. Biochem J 303, 941947.

GC Burdge , FJ Kelly & A Postle (1993 a) Mechanisms of hepatic phosphatidylcholine synthesis in the developing guinea pig: contributions of acyl remodelling and of N-methylation of phosphatidylethanolamine. Biochem J 290, 6773.

GC Burdge , FJ Kelly & AD Postle (1993) Synthesis of phosphatidylcholine in guinea-pig fetal lung involves acyl remodelling and differential turnover of individual molecular species. Biochim Biophys Acta 1166, 251257.

GC Burdge & AD Postle (1995) Phospholipid molecular species composition of developing fetal guinea pig brain. Lipids 30, 719724.

PC Calder (2001) Polyunsaturated fatty acids, inflammation and immunity. Lipids 36, 10071024.

YM Heung & AD Postle (1995) The molecular selectivity of phospholipase D in HL60 granulocytes. FEBS Lett 364, 250254.

SM Innis (1991) Essential fatty acids in growth and development. Prog Lipid Res 30, 39103.

B Koletzko & M Braun (1991) Arachidonic acid and early human growth: is there a relation? Ann Nutr Metab 35, 128131.

SC Langley & AA Jackson (1994) Increased systolic blood pressure in adult rats induced by foetal exposure to maternal low protein diet. Clin Sci 86, 217222.

SC Langley-Evans & M Nwagwu (1998) Impaired growth and increased glucocorticoid-sensitive enzyme activities in tissues for rat fetuses exposed to maternal low protein diets. Life Sci 63, 605615.

SC Langley-Evans , SJ Whelham , RC Sherman & AA Jackson (1996) Weanling rats exposed to maternal low-protein diets during discrete periods of gestation exhibit differing severity of hypertension. Clin Sci 91, 607615.

AA Leaf , MJ Leighfield , KL Costeloe & MA Crawford (1992) Long chain polyunsaturated fatty acids and fetal growth. Early Hum Dev 30, 183191.

WMF Leat , R Curtis , NJ Millicham & RW Cox (1986) Retinal function in rats and guinea-pigs reared on diets low in essential fatty acids and supplemented with linoleic or linolenic acids. Ann Nutr Metab 30, 166174.

DS Lin , WE Connor , GJ Anderson & M Neuringer (1990) Effects of dietary n-3 fatty acids on the phospholipid molecular species of monkey brain. J Neurochem 55, 12001207.

BJ Litman & DC Mitchell (1996) A role for phospholipid polyunsaturation in modulating membrane protein function. Lipids 31, Suppl., S193S197.

DC Mitchell , M Straume & BJ Litman (1992) Role of sn-1 saturated, sn-2 polyunsaturated phospholipids in control of membrane receptor conformational equilibrium: effects of cholesterol and acyl chain unsaturation on the metarhodopsin IΦ metarhodopsin II equilibrium. Biochemistry 31, 662670.

M Neuringer , GJ Anderson & WE Connor (1988) The essentiality of n-3 fatty acids for the development and function of the retina and brain. Annu Rev Nutr 8, 517541.

M Neuringer , WE Connor , DS Lin , L Barstad & S Luck (1986) Biochemical and functional effects of prenatal and postnatal omega-3 fatty acid deficiency on retina and brain in rhesus monkeys. Proc Natl Acad Sci USA 83, 40214025.

M Neuringer , WE Connor , C Van Petten & L Barstad (1984) Dietary omega-3 fatty acid deficiency and visual loss in infant rhesus monkeys. J Clin Invest 73, 272276.

SE Ozanne , ND Martinsz , CJ Petry , CL Loizou & CN Hales (1998) Maternal low protein diet in rats programmes fatty acids desaturase activities in the offspring. Diabetologica 41, 13371342.

S Reisbick , M Neuringer , R Hasnain & WE Connor (1990) Polydipsia in rhesus monkeys deficient in omega-3 fatty acids. Physiol Behav 47, 315323.

S Reisbick , M Neuringer , R Hasnain & WE Connor (1994) Home cage behavior of rhesus monkeys with long-term deficiency of omega-3 fatty acids. Physiol Behav 55, 231239.

N Salem Jr & CD Niebylski (1995) The nervous system has an absolute molecular species requirement for proper function. Mol Membr Biol 12, 131134.

P Sanchez-Pinera , V Micol , S Corbalan-Garcia & JC Gomez-Fernandez (1999) A comprehensive study of the activation of protein kinase C alpha by different diacylglycerol isomers. Biochem J 337, 387395.

AJ Sinclair & MA Crawford (1972) The accumulation of arachidonate and docosahexaenoate in the developing rat brain. J Neurochem 19, 17531758.

EN Smit , JM Dijkstra , TA Schnater , E Seerat , FAJ Muskiet & ER Boersma (1997) Effects of malnutrition on the erythrocyte fatty acid composition and plasma vitamin E levels of Pakistani children. Acta Paediatrica 86, 690695.

EN Smit , HA Woltil , ER Boersma & FAJ Muskkiet (1999) Low erythrocyte docosahexaenoic acid in malnourished, often breast-fed, Pakistani infants. A matter of concern? Eur J Pediatr 158, 525

H Sprecher (2000) Metabolism of highly unsaturated n-3 and n-6 fatty acids. Biochim Biophys Acta 1486, 219231.

HM Su , LA Keswick & JT Brenna (1996) Increasing dietary linolenic acid in young rats increases and then decreases docosahexaenoic acid in retina but not brain. Lipids 31, 12891298.

LB Tijburg , RW Samborski & DE Vance (1991) Evidence that remodeling of the fatty acids of phosphatidylcholine is regulated in isolated rat hepatocytes and involves both the sn-1 and sn-2 positions. Biochim Biophys Acta 1085, 184190.

HS Weisinger , JA Armitage , AJ Sinclair , AJ Vingrys , PL Burns & RS Weisinger (2001) Perinatal omega-3 fatty acid deficiency affects blood pressure later in life. Nat Med 7, 258259.

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