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Effect of ruminal administration of Escherichia coli wild type or a genetically modified strain with enhanced high nitrite reductase activity on methane emission and nitrate toxicity in nitrate-infused sheep

Published online by Cambridge University Press:  08 March 2007

C. Sar ,
B. Mwenya ,
B. Pen ,
K. Takaura ,
R. Morikawa ,
A. Tsujimoto ,
K. Kuwaki ,
N. Isogai ,
I. Shinzato  and
Y. Asakura
...Show all authors
C. Sar
Affiliation:
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro Hokkaido, 080-8555, Japan
B. Mwenya
Affiliation:
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro Hokkaido, 080-8555, Japan
B. Pen
Affiliation:
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro Hokkaido, 080-8555, Japan
K. Takaura
Affiliation:
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro Hokkaido, 080-8555, Japan
R. Morikawa
Affiliation:
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro Hokkaido, 080-8555, Japan
A. Tsujimoto
Affiliation:
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro Hokkaido, 080-8555, Japan
K. Kuwaki
Affiliation:
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro Hokkaido, 080-8555, Japan
N. Isogai
Affiliation:
Fermentation and Biotechnology Laboratories, Global Foods and Amino Acids Company, Ajinomoto Co. Inc., 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki-shi, 210-8681, Japan
I. Shinzato
Affiliation:
Fermentation and Biotechnology Laboratories, Global Foods and Amino Acids Company, Ajinomoto Co. Inc., 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki-shi, 210-8681, Japan
Y. Asakura
Affiliation:
Fermentation and Biotechnology Laboratories, Global Foods and Amino Acids Company, Ajinomoto Co. Inc., 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki-shi, 210-8681, Japan
Y. Toride
Affiliation:
Fermentation and Biotechnology Laboratories, Global Foods and Amino Acids Company, Ajinomoto Co. Inc., 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki-shi, 210-8681, Japan
J. Takahashi*
Affiliation:
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro Hokkaido, 080-8555, Japan
*
*Corresponding author: Professor J. Takahashi, fax +81 155 49 5421, email junichi@obihiro.ac.jp
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Abstract

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The effects of two kinds of Escherichia coli (E. coli) strain, wild-type E. coli W3110 and E. coli nir-Ptac, which has enhanced NO2 reduction activity, on oral CH4 emission and NO3 toxicity in NO3-treated sheep were assessed in a respiratory hood system in a 4×6 Youden square design. NO3 (1·3g NaNO3/kg0·75 body weight) and/or E. coli strains were delivered into the rumen through a fistula as a single dose 30min after the morning meal. Escherichia coli cells were inoculated for sheep to provide an initial E. coli cell density of optical density at 660nm of 2, which corresponded to 2×1010 cells/ml. The six treatments consisted of saline, E. coli W3110, E. coli nir-Ptac, NO3, NO3 plus E. coli W3110, and NO3 plus E. coli nir-Ptac. CH4 emission from sheep was reduced by the inoculation of E. coli W3110 or E. coli nir-Ptac by 6% and 12%, respectively. NO3 markedly inhibited CH4 emission from sheep. Compared with sheep given NO3 alone, the inoculation of E. coli W3110 to NO3-infused sheep lessened ruminal and plasma toxic NO2 accumulation and blood methaemoglobin production, while keeping ruminal methanogenesis low. Ruminal and plasma toxic NO2 accumulation and blood methaemoglobin production in sheep were unaffected by the inoculation of E. coli nir-Ptac. These results suggest that ruminal methanogenesis may be reduced by the inoculation of E. coli W3110 or E. coli nir-Ptac. The inoculation of E. coli W3110 may abate NO3 toxicity when NO3 is used to inhibit CH4 emission from ruminants.

Keywords

Escherichia coli W3110Escherichia coli nir-PtacMethane emissionNitrate
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 5 , November 2005 , pp. 691 - 697
Copyright
Copyright © The Nutrition Society 2005

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