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Effect of starch on the cariogenic potential of sucrose

  • Cecilia C. C. Ribeiro (a1), Cínthia P. M. Tabchoury (a1), Altair A. Del Bel Cury (a1), Livia M. A. Tenuta (a1), Pedro L. Rosalen (a1) and Jaime A. Cury (a1)...

Abstract

Since in vitro and animal studies suggest that the combination of starch with sucrose may be more cariogenic than sucrose alone, the study assessed in situ the effects of this association applied in vitro on the acidogenicity, biochemical and microbiological composition of dental biofilm, as well as on enamel demineralization. During two phases of 14 d each, fifteen volunteers wore palatal appliances containing blocks of human deciduous enamel, which were extra-orally submitted to four groups of treatments: water (negative control, T1); 2 % starch (T2); 10 % sucrose (T3); and 2 % starch+10 % sucrose (T4). The solutions were dripped onto the blocks eight times per day. The biofilm formed on the blocks was analysed with regard to amylase activity, acidogenicity, and biochemical and microbiological composition. Demineralization was determined on enamel by cross-sectional microhardness. The greatest mineral loss was observed for the association starch+sucrose (P<0·05). Also, this association resulted in the highest lactobacillus count in the biofilm formed (P<0·05). In conclusion, the findings suggest that a small amount of added starch increases the cariogenic potential of sucrose.

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Corresponding author

*Corresponding author: Professor Jaime A. Cury, fax +55 19 3412 5218, email jcury@fop.unicamp.br

References

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Bernfeld, P (1955) Amylases α and β. Meth Enzymol 1, 149159.
Bowen, WH, Amsbaugh, SM, Monell-Torrens, S, Brunelle, J, Kuzmiak-Jones, H & Cole, MF (1980) A method to assess cariogenic potential of foodstuffs. J Am Dent Assoc 100, 677681.
Bowen, WH, Pearson, S, Young, D & Thibodeau, E (1986) The effect of partial desalivation on coronal and root surface caries in the rat. In Factors Relating to Demineralization and Remineralization of the Teeth, pp.243250 [Leach, SA, editor].Oxford: IRL Press.
Box, GEP, Hunter, WG & Hunter, JS (1978) Statistics for Experimenters. New York: John Wiley & Sons Inc.
Cury, JA, Hashizume, LN, Del Bel Cury, AA & Tabchoury, CPM (2001) Effects of dentifrice containing fluoride and/or baking soda on enamel demineralization/remineralization: an in situ study. Caries Res 35, 106110.
Cury, JA, Rebello, MA & Del Bel Cury, AA (1997) In situ relationship between sucrose exposure and the composition of dental plaque. Caries Res 31, 356360.
Cury, JA, Rebelo, MAB, Del Bel Cury, AA, Derbyshire, MTVC & Tabchoury, CPM (2000) Biochemical composition and cariogenicity of dental plaque formed in the presence of sucrose or glucose and fructose. Caries Res 34, 491497.
Dibdin, GH & Shellis, RP (1988) Physical and biochemical studies of Streptococcus mutans sediments suggest new factors linking the cariogenicity of plaque with its extracellular polysaccharide content. J Dent Res 67, 890895.
Dodds, MWJ & Edgar, WM (1986) Effects of dietary sucrose levels on pH fall and acid–anion profile in human dental plaque after a starch mouth-rinse. Arch Oral Biol 31, 509512.
Featherstone, JDB, ten Cate, JM, Shariati, M & Arends, J, (1983) Comparison of artificial caries-like lesions by quantitative microradiography and microhardness profiles. Caries Res 17, 385391.
Firestone, AR, Schmid, R & Mühlemann, HR (1982) Cariogenic effects of cooked wheat starch alone or with sucrose and frequency-controlled feedings in rats. Arch Oral Biol 27, 759763.
Fisher, FJ (1968) A field survey of dental caries, periodontal disease and enamel defects in Tristan da Cunha. Br Dent J 125, 447453.
Gold, OG, Jordan, HV & Van Houte, J (1973) A selective medium for Streptococcus mutans. Arch Oral Biol 18, 13571364.
Green, RM & Hartles, RL (1967) The effect of uncooked and roll-dried maize starch, alone and mixed in equal quantity with sucrose, on dental caries in the albino rat. Br J Nutr 21, 225230.
Gustaffson, BE, Quensel, CE, Lanke, LS, Lundqvist, C, Grahnen, H, Bonow, BE & Krasse, B (1954) The Vipeholm dental caries study; the effect of different levels of carbohydrate intake on caries activity in 436 individuals observed for five years. Acta Odontol Scand 11, 232264.
Hara, AT, Queiroz, CS, Paes Leme, AF, Serra, MC & Cury, JA (2003) Caries progression and inhibition in human and bovine root dentine in situ. Caries Res 37, 339344.
Hefti, A & Schmid, T (1979) Effect on caries incidence in rats of increasing dietary sucrose levels. Caries Res 13, 298300.
Imfeld, T (1977) Evaluation of the cariogenicity of confectionery by intra-oral wire-telemetry. SSO Schweiz Monatsschr Zahnheilkd 87, 437464.
König, KG & Grenby, TH (1965) The effect of wheat grain fractions and sucrose mixtures on rat caries developing in two strains of rats maintained on different regimes and evaluated by two different methods. Arch Oral Biol 10, 143153.
Kopec, LK, Vacca-Smith, AM & Bowen, WH (1997) Structural aspects of glucans formed in solution and on the surface of hydroxyapatite. Glycobiology 7, 929934.
Larsen, MJ & Pearce, EI (1997) A computer program for correlating dental plaque pH values, cH +, plaque titration, critical pH, resting pH and the solubility of enamel apatite. Arch Oral Biol 42, 475480.
Lingström, P, Birkhed, D, Ruben, J & Arends, J (1994) Effect of frequent consumption of starchy food items on enamel and dentine demineralization and on plaque pH in situ. J Dent Res 73, 652660.
Lingström, P, Holm, J, Birkhed, D & Björck, I (1989) Effects of variously processed starch on pH of human dental plaque. Scand J Dent Res 97, 392400.
Lingström, P, Van Houte, J & Kashket, S (2000) Food starches and dental caries. Crit Rev Oral Biol Med 11, 366380.
Marsh, PD (1994) Microbial ecology of dental plaque and its significance in health and disease. Adv Dent Res 8, 263271.
Marthaler, TM & Froesch, ER (1967) Deficient dental caries attack in persons with hereditary fructose intolerance. Hel Med Acta Suppl 47, 126.
Mattos-Graner, RO, Smith, DJ, King, WF & Mayer, MPA (2000) Water-insoluble glucan synthesis by mutans streptococcal strains correlates with caries incidence in 12- to 30-month-old children. J Dent Res 79, 13711377.
Mattos-Graner, RO, Zelante, F, Line, RC & Mayer, M (1998) Association between caries prevalence and clinical, microbiological and dietary variables in 1·0 to 2·5-year-old Brazilian children. Caries Res 32, 319323.
Mundorff-Shrestha, SA, Featherstone, JD, Eisenberg, AD, Cowles, E, Curzon, ME, Espeland, MA & Shields, CP (1994) Cariogenic potential of foods. II. Relationship of food composition, plaque microbial counts, and salivary parameters to caries in the rat model. Caries Res 28, 106115.
Newbrun, E, Hoover, C, Mettraux, G & Graf, H (1980) Comparison of dietary habits and dental health of subjects with hereditary fructose intolerance and control subjects. J Am Dent Assoc 101, 619626.
Nobre, Dos Santos M, Melo, Dos Santos L, Francisco, SB & Cury, JA (2002) Relationship among dental plaque composition, daily sugar exposure and caries in the primary dentition. Caries Res 36, 347352.
Paes Leme, AF, Dalcico, R, Tabchoury, CP, Del Bel Cury, AA, Rosalen, PL & Cury, JA (2004) In situ effect of frequent sucrose exposure on enamel demineralization and on plaque composition after APF application and F dentifrice use. J Dent Res 83, 7175.
Pecharki, GD, Cury, JA, Paes Leme, AF, Tabchoury, CP, Del Bel Cury, AA, Rosalen, PL & Bowen, WH (2005) Effect of sucrose containing iron (II) on dental biofilm and enamel demineralization in situ. Caries Res 39, 123129.
Rölla, G, Scheie, AA & Ciardi, JE (1985) Role of sucrose in plaque formation. Scand J Dent Res 93, 105111.
Stephan, RM (1940) Changes in hydrogen-ion concentration on tooth surfaces and in caries lesion. J Am Dent Assoc 27, 718723.
Vacca-Smith, AM, Venkitaraman, AR, Quivey, RG Jr & Bowen, WH (1996) Interactions of streptococcal glucosyltransferases with α-amylase and starch on the surface of saliva-coated hydroxyapatite. Arch Oral Biol 41, 291298.
White, DJ & Featherstone, JDB (1987) A longitudinal microhardness analysis of fluoride dentifrice effects on lesion progression in vivo. Caries Res 21, 502512.
Zylber, LJ & Jordan, H (1982) Development of a selective medium for detection and enumeration of Actinomyces viscosus and Actinomyces naeslundii in dental plaque. J Clin Microbiol 15, 253259.

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