Hostname: page-component-8448b6f56d-c47g7 Total loading time: 0 Render date: 2024-04-23T21:07:54.560Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of conjugated linoleic acid on linoleic and linolenic acid metabolism in man

Published online by Cambridge University Press:  08 March 2007

Anu M. Turpeinen ,
Sonja Bärlund ,
Riitta Freese ,
Peter Lawrence  and
Thomas Brenna
Anu M. Turpeinen*
Affiliation:
Department of Applied Chemistry and Microbiology (Nutrition), University of Helsinki, PO Box 66, 00014 University of Helsink, Finland
Sonja Bärlund
Affiliation:
Department of Applied Chemistry and Microbiology (Nutrition), University of Helsinki, PO Box 66, 00014 University of Helsink, Finland
Riitta Freese
Affiliation:
Department of Applied Chemistry and Microbiology (Nutrition), University of Helsinki, PO Box 66, 00014 University of Helsink, Finland
Peter Lawrence
Affiliation:
Division of Nutritional Sciences, Cornell University, Ithaca, NY, USA
Thomas Brenna
Affiliation:
Division of Nutritional Sciences, Cornell University, Ithaca, NY, USA
*
*Corresponding author: Dr Anu Turpeinen, fax +358 9 19158269, email anu.turpeinen@helsinki.fi
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Evidence from animal studies suggests that conjugated linoleic acid (CLA) modulates plasma and tissue appearance of newly synthesized PUFA. The effects of a 1·2g (0·5% energy) daily intake of the cis-9, trans-11 (c9, t11) isomer of CLA, trans-10,cis-12 (t10, c12) isomer of CLA or olive oil (placebo) on linoleic acid (LA) and linolenic acid (LNA) metabolism in healthy human volunteers was investigated. Fifteen subjects were fed an experimental diet and supplemented with c9,t11-CLA, t10, c12-CLA or placebo for 7d before consuming a tracer dose of U-[13C]La (50%Mg) And U-[13C]Lna (50Mg). Blood Samples Were Taken At 0, 2, 4, 6, 8, 24, 48, 72 and 168%h and analysed using high-precision MS. No differences between the groups in peak plasma [13C]LA (10·3–11·6% of dose), [13C]LNA (2·5–2·9% of dose), [13C]arachidonic acid (0·09–0·12% of dose), [13C]EPA (0·04–0·06% of dose) or [13C]Dha (0c·06–0·10% of dose) were detected. Concentration V. time curves (area under the curve) also showed no significant differences between groups. This suggests that, in healthy human subjects consuming a diet with adequate intake of essential fatty acids, CLA does not affect metabolism of LA or LNA

Keywords

Conjugated linoleic acidLinoleic acidLinolenic acidStable isotopeMan
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 4 , April 2006 , pp. 727 - 733
Copyright
Copyright © The Nutrition Society 2006

References

Aro, A, Männistä, S, Salminen, I, Ovaskainen, M-L, Kataja, V & Uusitupa, MInverse association between dietary and serum conjugated linoleic acid and risk of breast cancer in postmenopausal women. Nutr Cancer (2000) 38, 151–157.CrossRefGoogle ScholarPubMed
Badinga, L, Selberg, KT, Dinges, AC, Corner, CW & Miles, RDDietary conjugated linoleic acid alters hepatic lipid content and fatty acid composition in broiler chickens. Poult Sci (2003) 82, 111–116.CrossRefGoogle ScholarPubMed
Banni, S, Angioni, E, Carta, Get al.. Influence of dietary conjugated linoleic acid on lipid metabolism in relation to its anticarcinogenic activity. In Advances in Conjugated Linoleic Acid Research, vol. 1, pp. 307–318 [Yurawecz, MP, Mossoba, MM, Kramer, JKG, Pariza, MWNelson, GJ, editor]. Champaign, IL: AOCS Press. (1999 a)Google Scholar
Banni, S, Angioni, E, Casu, V, Melis, MP, Carta, G, Corongiu, FP, Thompson, H & Ip, CDecrease in linoleic acid metabolites as a potential mechanism in cancer risk reduction by conjugated linoleic acid. Carcinogenesis (1999 b) 20, 1019–1024.CrossRefGoogle ScholarPubMed
Banni, S, Carta, G, Angioni, E, Murru, EScanu, PMelis, MPBauman, DEFischer, SM & Ip, CDistribution of conjugated linoleic acid and metabolites in different lipid fractions in the rat liver. J Lipid Res (2001) 42, 1056–1061.CrossRefGoogle ScholarPubMed
Belury, MAInhibition of carcinogenesis by conjugated linoleic acid: potential mechanisms of action. J Nutr (2002) 132, 2995–2998.CrossRefGoogle ScholarPubMed
Belury, MAConjugated linoleic acid modulates hepatic lipid composition in mice. Lipids (1997) 32, 199–204.CrossRefGoogle ScholarPubMed
Benito, P, Nelson, GJ, Kelley, DS, Bartolini, G, Schmidt, PC & Simon, VThe effect of conjugated linoleic acid on platelet function, platelet fatty acid composition, and blood coagulation in humans. Lipids (2001 a) 36, 221–227.CrossRefGoogle ScholarPubMed
Benito, P, Nelson, GJ, Kelley, DS, Bartolini, GSchmidt, pc & Simon, VThe effect of conjugated linoleic acid on plasma lipoproteins and tissue fatty acid composition in humans. Lipids (2001 b) 36, 229–236.CrossRefGoogle ScholarPubMed
Bligh, E & Dyer, WA rapid method of total lipid extraction and purification. Can J Biochem Physiol (1959) 37, 911–917.CrossRefGoogle ScholarPubMed
Bretillon, L, Chardigny, JM, Gregoire, S, Bordeaux, O & Sebedio, JLEffects of conjugated linoleic acid isomers on the hepatic microsomal desaturation activities in vitro. Lipids (1999) 34, 965–969.CrossRefGoogle ScholarPubMed
Burdge, GC, Lupoli, B, Russell, JJ,et al. Incorporation of cis-9, trans-11 or trans-10, cis-12 conjugated linoleic acid into plasma and cellular lipids in healthy men. J Lipid Res (2004) 45, 736–741.CrossRefGoogle ScholarPubMed
Chuang, L-T, Thurmond, JM, Liu, Jw, Kirchner, SJ, Mukerji, PBray, TM & Huang, Y-SEffect of conjugated linoleic acid (CLA) on fungal delta-6-desaturase activity in a transformed yeast system. Lipids (2001 a) 36, 139–143.CrossRefGoogle Scholar
Chuang, L-T, Leonard, AE, Liu, J-W, Mukerji, P, Bray, TM & Huang, Y-SInhibitory effect of conjugated linoleic acid on linoleic acid elongation in transformed yeast with human elongase. Lipids (2001 b) 36, 1099–1103.CrossRefGoogle ScholarPubMed
Du, M, Ahn, DU & Sell, JLEffects of dietary conjugated linoleic acid and linoleic:linolenic acid ratio on polyunsaturated fatty acid status in laying hens. Poult Sci (2000) 79, 1749–1756.CrossRefGoogle ScholarPubMed
Emken, EA, Adlof, RO, Duval, S, Nelson, G & Benito, PEffect of dietary conjugated linoleic acid (CLA) on metabolism of isotope-labeled oleic, linoleic, and CLA isomers in women. Lipids (2002) 37, 741–750.CrossRefGoogle ScholarPubMed
Fremann, D, Linseisen, J & Wolfram, GDietary conjugated linoleic acid (CLA) intake assessment and possible biomarkers of CLA intake in young women. Public Health Nutr (2002) 5, 73–80.CrossRefGoogle ScholarPubMed
Goodman, KJ & Brenna, JTHigh sensitivity tracer detection using high-precision gas chromatography–combustion isotope ratio mass spectrometry and highly enriched (U-13C)-labeled precursors. Anal Chem (1992) 64, 1088–1095.CrossRefGoogle ScholarPubMed
Kramer, JK, Sehat, N, Dugan, ME, Mossoba, MM, Yurawecz, MP, Roach, JA, Eulitz, K, Aalhus, JL, Schaefer, AL & Ku, YDistributions of conjugated linoleic acid (CLA) isomers in tissue lipid classes of pigs fed a commercial CLA mixture determined by gas chromatography and silver ion-high-performance liquid chromatography. Lipids (1998) 33, 549–558.CrossRefGoogle ScholarPubMed
Li, Y & Watkins, BAConjugated linoleic acids alter bone fatty acid composition and reduce ex vivo prostaglandin E2 biosynthesis in rats fed n-6 or n-3 fatty acids. Lipids (1998) 33, 417–425.CrossRefGoogle ScholarPubMed
Loor, JJ, Lin, X & Herbein, JHEffects of dietary cis 9, trans 11–18:2, trans 10, cis 12–18:2, or vaccenic acid (trans 11–18:1) during lactation on body composition, tissue fatty acids profiles, and litter growth in mice. Br J Nutr (2003) 90, 1039–1048.CrossRefGoogle ScholarPubMed
Martin, JC, Gregoire, S, Siess, MH, Genty, M, Chardigny, JMBerdeaux, O, Juaneda, P & Sebedio, JLEffects of conjugated linoleic acid isomers on lipid metabolizing enzymes in male rats. Lipids (2000) 35, 91–98.CrossRefGoogle ScholarPubMed
Matthews, JNS, Altman, DG, Campbell, MJ & Royston, PAnalysis of serial measurements in medical research. BMJ (1990) 300, 230–235.CrossRefGoogle ScholarPubMed
Mougios, V, Matsakas, A, Petridou, A, Ring, S, Sagredos, A, Melissopoulou, A, Tsigilis, N & Nikolaidis, MEffect of supplementation with conjugated linoleic acid on human serum lipids and body fat. J Nutr Biochem (2001) 12, 585–594.CrossRefGoogle ScholarPubMed
Noone, EJ, Roche, HM, Nugent, AP & Gibney, MJThe effect of supplementation using isomeric blends of conjugated linoleic acid on lipid metabolism in healthy human subjects. Br J Nutr (2002) 88, 243–251.CrossRefGoogle ScholarPubMed
Nugteren, DH & Christ-Hazelhof, ENaturally-occurring conjugated octadecatrienoic acids are strong inhibitors of prostaglandin biosynthesis. Prostaglandins (1987) 33, 403–417.CrossRefGoogle ScholarPubMed
Ostrowska, E, Cross, RF, Muralitharan, M, Bauman, DE & Dunshea, FRDietary conjugated linoleic acid differentially alters fatty acid composition and increases conjugated linoleic acid content in porcine adipose tissue. Br J Nutr (2003) 90, 915–928.CrossRefGoogle ScholarPubMed
Pariza, MW, Park, Y & Cook, METhe biologically active isomers of conjugated linoleic acid. Prog Lipid Res (2001) 40, 283–298.CrossRefGoogle ScholarPubMed
Petridou, A, Mougios, V & Sagredos, ASupplementation with CLA: isomer incorporation into serum lipids and effect on body fat of women. Lipids (2003) 38, 805–811.CrossRefGoogle ScholarPubMed
Ramsay, TG, Evock-Clover, CM, Sttele, NC & Azain, MJDietary conjugated linoleic acid alters fatty acid composition in pig skeletal muscle and fat. J Anim Sci (2001) 79, 2152–2161.CrossRefGoogle ScholarPubMed
Ritzenthaler, KL, McGuire, MK, Falen, R, Shultz, TD, Dasgupta, N & McGuire, MAEstimation of conjugated linoleic acid intake by written dietary assessment methodologies under estimates actual intake evaluated by food duplicate methodology. J Nutr (2001) 131, 1548–1554.CrossRefGoogle Scholar
Scheaff, RC, Su, H-M, Keswick, LA & Brenna, JTConversion of a-linolenate to docosahexaenoate is not depressed by high dietary levels of linoleate in young rats: tracer evidence using high precision mass spectrometry. J Lipid Res (1995) 36, 998–1008.CrossRefGoogle Scholar
Sebedio, JL, Angioni, E, Chardigny, JM, Gregoire, SJuaneda, P & Berdeaux, OThe effect of conjugated linoleic acid isomers on fatty acid profiles of liver and adipose tissues and their conversion to isomers of 16:2 and 18:3 conjugated fatty acids in rats. Lipids (2001) 36, 575–582.CrossRefGoogle ScholarPubMed
Sebedio, JL, Chardigny, JM & Berdeaux, OMetabolism of conjugated linoleic acids. In Advances in Conjugated Linoleic Acid Research, pp. Vol.2 259–266 [Sebedio, JLChristie, WWAdlof, Reditor]. Champaign, IL: AOCS Press. (2003)Google Scholar
Turpeinen, AM, Mutanen, M, Aro, A, Salminen, I, Basu, S, Palmquist, DL & Griinari, JMBioconversion of vaccenic acid to conjugated linoleic acid in humans. Am J Clin Nutr (2002) 76, 504–510.CrossRefGoogle ScholarPubMed