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Extrinsic iron from soil contributes to Hb regeneration of anaemic rats: implications for foods contaminated with soil iron

  • Habtamu Guja (a1) and Kaleab Baye (a1)
Abstract

Contamination of foods with extrinsic (soil) Fe is common in developing countries. However, the bioavailability of this extrinsic Fe and the extent to which it contributes to Fe nutrition remains unknown. The present study compared the bioavailability of laboratory- and field-threshed teff (Eragrostisis tef (Zucc) Trotter) to evaluate the bioavailablity of extrinsic soil Fe that resulted from the traditional threshing of the staple grain. Using sequential extraction, Fe was fractionated and its solubility was evaluated. The contribution of the additional extrinsic (soil) Fe to the Hb regeneration of Fe-depleted rats was evaluated using a rat Hb depletion–repletion assay. Weanling male Wistar rats (n 24) were fed Fe-deficient diet for 21 d, and were then repleted for 14 d with diets: either laboratory-threshed teff (35 mg Fe/kg; n 8), field-threshed teff (35 mg intrinsic Fe/kg+ 120 mg soil Fe/kg; n 8), or FeSO4 (control; n 8). Fe content of field-threshed teff (29·4 mg/100 g) was four times greater than that of the laboratory-threshed (6·7 mg/100 g) teff (P<0·05). Soil contamination significantly increased the exchangeable, acid-soluble and reducible fractions obtained after sequential extraction. The relative biological value of the field-threshed teff (88 %) was higher than that of the laboratory-threshed (68 %) teff (P<0·05). Soil Fe can contribute to Hb regeneration in Fe-deficient rats. Considering that contamination of foods with soil is common in Ethiopia and other developing countries, it needs to be accounted for in the design and implementation of fortification programmes to prevent excessive intakes. Human studies are needed to confirm the present findings.

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Corresponding author
* Corresponding author: K. Baye, email kaleabbaye@gmail.com
References
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1. Lopez, A, Cacoub, P, Macdougall, IC, et al. (2016) Iron deficiency anaemia. Lancet 387, 907916.
2. Camaschella, C (2015) Iron-deficiency anemia. N Engl J Med 372, 18321843.
3. Baye, K, Guyot, J-P, Icard-Vernière, C, et al. (2015) Enzymatic degradation of phytate, polyphenols and dietary fibers in Ethiopian injera flours: effect on iron bioaccessibility. Food Chem 174, 6067.
4. Hurrell, R & Egli, I (2010) Iron bioavailability and dietary reference values. Am J Clin Nutr 91, 1461S1467S.
5. Schauer, C & Zlotkin, S (2003) Home fortification with micronutrient sprinkles – a new approach for the prevention and treatment of nutritional anemias. Paediatr Child Health 8, 8790.
6. World Health Organization (2011) Guideline: Use of Multiple Micronutrient Powders for Home Fortification of Foods Consumed by Infants and Children 6–23 Months of Age. Geneva: WHO.
7. Baye, K, Guyot, J-P, Icard-Verniere, C, et al. (2013) Nutrient intakes from complementary foods consumed by young children (aged 12–23 months) from North Wollo, northern Ethiopia: the need for agro-ecologically adapted interventions. Public Health Nutr 16, 17411750.
8. Abebe, Z, Haki, GD & Baye, K (2017) Simulated effects of home fortification of complementary foods with micronutrient powders on risk of inadequate and excessive intakes in West Gojjam, Ethiopia. Matern Child Nutr 14, e12443.
9. Abebe, Y, Bogale, A, Hambidge, KM, et al. (2007) Phytate, zinc, iron and calcium content of selected raw and prepared foods consumed in rural Sidama, Southern Ethiopia, and implications for bioavailability. J Food Comp Anal 20, 161168.
10. Baye, K, Mouquet‐Rivier, C, Icard‐Vernière, C, et al. (2014) Changes in mineral absorption inhibitors consequent to fermentation of Ethiopian injera: implications for predicted iron bioavailability and bioaccessibility. Int J Food Sci Technol 49, 174180.
11. Icard-Vernière, Cl, Hama, F, Guyot, J-P, et al. (2013) Iron contamination during in-field milling of millet and sorghum. J Agric Food Chem 61, 1037710383.
12. Greffeuille, V, Kayodé, AP, Icard-Vernière, C, et al. (2011) Changes in iron, zinc and chelating agents during traditional African processing of maize: effect of iron contamination on bioaccessibility. Food Chem 126, 18001807.
13. Adish, AA, Esrey, SA, Gyorkos, TW, et al. (1999) Effect of consumption of food cooked in iron pots on iron status and growth of young children: a randomised trial. Lancet 353, 712716.
14. Geerligs, P, Brabin, B & Omari, A (2003) Food prepared in iron cooking pots as an intervention for reducing iron deficiency anaemia in developing countries: a systematic review. J Hum Nutr Diet 16, 275281.
15. Gibbs, MM, Carriquiry, AL, Capanzana, MV, et al. (2014) Establishing desirable fortificant levels for calcium, iron and zinc in foods for infant and young child feeding: examples from three Asian countries. Matern Child Nutr 10, 112125.
16. Allen, LH, De Benoist, B, Dary, O, et al. (2006) Guidelines on Food Fortification with Micronutrients. Geneva: WHO.
17. Tessier, A, Campbell, PG & Bisson, M (1979) Sequential extraction procedure for the speciation of particulate trace metals. Anal Chem 51, 844851.
18. Forbes, AL, Arnaud, M, Chichester, C, et al. (1989) Comparison of in vitro, animal, and clinical determinations of iron bioavailability: International Nutritional Anemia Consultative Group Task Force report on iron bioavailability. Am J Clin Nutr 49, 225238.
19. Jorhem, L (2000) Determination of metals in foods by atomic absorption spectrometry after dry ashing: NMKL1 collaborative study. J AOAC Int 83, 12041211.
20. Simpson, RJ, Sidhar, S & Peters, TJ (1992) Application of selective extraction to the study of iron species present in diet and rat gastrointestinal tract contents. Br J Nutr 67, 437444.
21. Reeves, PG, Nielsen, FH & Fahey, GC Jr (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123, 19391951.
22. Fluttert, M, Dalm, S & Oitzl, MS (2000) A refined method for sequential blood sampling by tail incision in rats. Lab Anim 34, 372378.
23. Whittaker, P, Mahoney, AW & Hendricks, DG (1984) Effect of iron-deficiency anemia on percent blood volume in growing rats. J Nutr 114, 11371142.
24. National Research Council (2011) Guide for the Care and Use of Laboratory Animals. Washington, DC: National Academies Press.
25. Harvey, PW, Dexter, PB & Darnton-Hill, I (2000) The impact of consuming iron from non-food sources on iron status in developing countries. Public Health Nutr 3, 375383.
26. Karakochuk, CD, Murphy, HM, Whitfield, KC, et al. (2015) Elevated levels of iron in groundwater in Prey Veng province in Cambodia: a possible factor contributing to high iron stores in women. J Water Health 13, 575586.
27. Gibson, RS, Wawer, AA, Fairweather-Tait, SJ, et al. (2015) Dietary iron intakes based on food composition data may underestimate the contribution of potentially exchangeable contaminant iron from soil. J Food Comp Anal 40, 1923.
28. Hooda, P, Henry, C, Seyoum, T, et al. (2004) The potential impact of soil ingestion on human mineral nutrition. Sci Total Environ 333, 7587.
29. Lindsay, W & Schwab, A (1982) The chemistry of iron in soils and its availability to plants. J Plant Nutr 5, 821840.
30. Abbaspour, N, Hurrell, R & Kelishadi, R (2014) Review on iron and its importance for human health. J Res Med Sci 19, 164.
31. Gashu, D, Stoecker, BJ, Adish, A, et al. (2016) Ethiopian pre-school children consuming a predominantly unrefined plant-based diet have low prevalence of iron-deficiency anaemia. Public Health Nutr 19, 18341841.
32. Abebe, Y, Bogale, A, Hambidge, KM, et al. (2008) Inadequate intakes of dietary zinc among pregnant women from subsistence households in Sidama, Southern Ethiopia. Public Health Nutr 11, 379386.
33. Zimmermann, MB & Hilty, FM (2011) Nanocompounds of iron and zinc: their potential in nutrition. Nanoscale 3, 23902398.
34. Hilty, FM, Arnold, M, Hilbe, M, et al. (2010) Iron from nanocompounds containing iron and zinc is highly bioavailable in rats without tissue accumulation. Nat Nanotechnol 5, 374380.
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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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