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Feeding dried purple laver (nori) to vitamin B12-deficient rats significantly improves vitamin B12 status

  • Shigeo Takenaka (a1), Sumi Sugiyama (a1), Shuhei Ebara (a2), Emi Miyamoto (a3), Katsuo Abe (a3), Yoshiyuki Tamura (a1), Fumio Watanabe (a3), Shingo Tsuyama (a4) and Yoshihisa Nakano (a2)
  • DOI:
  • Published online: 01 March 2007

To clarify the bioavailability of vitamin B12 in lyophylized purple laver (nori; Porphyra yezoensis), total vitamin B12 and vitamin B12 analogue contents in the laver were determined, and the effects of feeding the laver to vitamin B12-deficient rats were investigated. The amount of total vitamin B12 in the dried purple laver was estimated to be 54.5 and 58.6 (SE 5.3 and 7.5 respectively) ΜG/100 g dry weight by lactobacillus bioassay and chemiluminescent assay with hog intrinsic factor respectively. the purple laver contained five types of biologically active vitamin b12 compounds (cyano-, hydroxo-, sulfito-, adenosyl- and methylcobalamin), in which the vitamin b12 coezymes (adenosyl- and methylcobalamin) comprised about 60 % of the total vitamin b12. when 9-week-old vitamin b12-deficient rats, which excreted substantial amounts of methylmalonic acid (71.7(se 20.2) μmol/d) in urine, were fed the diet supplemented with dried purple laver (10 μg/kg diet) for 20 d, urinary methylmalonic acid excretion (as an index of vitamin B12 deficiency) became undetectable and hepatic vitamin B12 (especially adenosylcobalamin) levels were significantly increased. These results indicate that vitamin B12 in dried purple laver is bioavailable to rats.

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Corresponding author
*Corresponding author: Dr Shigeo Takenaka, present address Department of Veterinary Science, Osaka Prefecture University, Sakai, Osaka, 599-8531, Japan, fax + 81 722 54 9489, email
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British Journal of Nutrition
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