Skip to main content Accesibility Help

Impact of fish oils on the outcomes of a mouse model of acute Pseudomonas aeruginosa pulmonary infection

  • Emilie Caron (a1), Jean-Luc Desseyn (a1), Luce Sergent (a2), Nana Bartke (a3), Marie-Odile Husson (a1), Alain Duhamel (a4) and Frédéric Gottrand (a1)...

Pseudomonas aeruginosa is an opportunistic Gram-negative bacterium that causes pneumonia in immunocompromised humans and severe pulmonary damage in patients with cystic fibrosis. Imbalanced fatty acid incorporation in membranes, including increased arachidonic acid and decreased DHA concentrations, is known to play a critical role in chronic inflammation associated with bacterial infection. Other lipids, such as EPA and alkylglycerols, are also known to play a role in inflammation, particularly by stimulating the immune system, decreasing inflammation and inhibiting bacterial growth. In this context, the goal of the present study was to assess the effect of dietary DHA/EPA, in a 2:1 ratio, and alkylglycerols, as natural compounds extracted from oils of rays and chimeras, respectively, on the inflammatory reaction induced by P. aeruginosa pulmonary infection in mice. To this end, mice were fed with a control diet or isolipidic, isoenergetic diets prepared with oils enriched in DHA/EPA (2:1) or alkylglycerols for 5 weeks before the induction of acute P. aeruginosa lung infection by endotracheal instillation. In our model, DHA/EPA (2:1) significantly improved the survival of mice after infection, which was associated with the acceleration of bacterial clearance and the resolution of inflammation leading to the improvement of pulmonary injuries. By contrast, alkylglycerols did not affect the outcomes of P. aeruginosa infection. Our findings suggest that supplementation with ray oil enriched in DHA/EPA (2:1) can be considered as a preventive treatment for patients at risk for P. aeruginosa infection.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the or variations. ‘’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Impact of fish oils on the outcomes of a mouse model of acute Pseudomonas aeruginosa pulmonary infection
      Available formats
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Impact of fish oils on the outcomes of a mouse model of acute Pseudomonas aeruginosa pulmonary infection
      Available formats
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Impact of fish oils on the outcomes of a mouse model of acute Pseudomonas aeruginosa pulmonary infection
      Available formats
Corresponding author
* Corresponding author: F. Gottrand, fax +33 320446134, email
Hide All
1 Oliver, A, Canton, R, Campo, P, et al. (2000) High frequency of hypermutable Pseudomonas aeruginosa in cystic fibrosis lung infection. Science 288, 12511254.
2 Davies, JC (2002) Pseudomonas aeruginosa in cystic fibrosis: pathogenesis and persistence. Paediatr Respir Rev 3, 128134.
3 Cantin, A (1995) Cystic fibrosis lung inflammation: early, sustained, and severe. Am J Respir Crit Care Med 151, 939941.
4 Lyczak, JB, Cannon, CL & Pier, GB (2002) Lung infections associated with cystic fibrosis. Clin Microbiol Rev 15, 194222.
5 Davis, PB, Drumm, M & Konstan, MW (1996) Cystic fibrosis. Am J Respir Crit Care Med 154, 12291256.
6 Miles, EA & Calder, PC (2012) Influence of marine n-3 polyunsaturated fatty acids on immune function and a systematic review of their effects on clinical outcomes in rheumatoid arthritis. Br J Nutr 107, Suppl. 2, S171S184.
7 Pierre, M, Husson, MO, Le Berre, R, et al. (2007) Omega-3 polyunsaturated fatty acids improve host response in chronic Pseudomonas aeruginosa lung infection in mice. Am J Physiol Lung Cell Mol Physiol 292, L1422L1431.
8 Tiesset, H, Pierre, M, Desseyn, JL, et al. (2009) Dietary (n-3) polyunsaturated fatty acids affect the kinetics of pro- and antiinflammatory responses in mice with Pseudomonas aeruginosa lung infection. J Nutr 139, 8289.
9 Anderson, BM & Ma, DW (2009) Are all n-3 polyunsaturated fatty acids created equal? Lipids Health Dis 8, 33.
10 Yamamoto, N, St Claire, DA Jr, Homma, S, et al. (1988) Activation of mouse macrophages by alkylglycerols, inflammation products of cancerous tissues. Cancer Res 48, 60446049.
11 Homma, S, Millman, I & Yamamoto, N (1990) A serum factor for macrophage activation after in vitro dodecylglycerol treatment of mouse lymphocytes. Immunol Cell Biol 68, 137142.
12 Ngwenya, BZ, Fiavey, NP & Mogashoa, MM (1991) Activation of peritoneal macrophages by orally administered ether analogues of lysophospholipids. Proc Soc Exp Biol Med 197, 9197.
13 Mitre, R, Etienne, M, Martinais, S, et al. (2005) Humoral defence improvement and haematopoiesis stimulation in sows and offspring by oral supply of shark-liver oil to mothers during gestation and lactation. Br J Nutr 94, 753762.
14 Ved, HS, Gustow, E, Mahadevan, V, et al. (1984) Dodecylglycerol. A new type of antibacterial agent which stimulates autolysin activity in Streptococcus faecium ATCC 9790. J Biol Chem 259, 81158121.
15 Brissette, JL, Cabacungan, EA & Pieringer, RA (1986) Studies on the antibacterial activity of dodecylglycerol. Its limited metabolism and inhibition of glycerolipid and lipoteichoic acid biosynthesis in Streptococcus mutans BHT. J Biol Chem 261, 63386345.
16 Reeves, PG, Rossow, KL & Lindlauf, J (1993) Development and testing of the AIN-93 purified diets for rodents: results on growth, kidney calcification and bone mineralization in rats and mice. J Nutr 123, 19231931.
17 Phillipson, M & Kubes, P (2011) The neutrophil in vascular inflammation. Nat Med 17, 13811390.
18 Krysko, O, Vandenabeele, P, Krysko, DV, et al. (2010) Impairment of phagocytosis of apoptotic cells and its role in chronic airway diseases. Apoptosis 15, 11371146.
19 Armstrong, L, Jordan, N & Millar, A (1996) Interleukin 10 (IL-10) regulation of tumour necrosis factor α (TNF-α) from human alveolar macrophages and peripheral blood monocytes. Thorax 51, 143149.
20 Cox, G (1996) IL-10 enhances resolution of pulmonary inflammation in vivo by promoting apoptosis of neutrophils. Am J Physiol 271, L566L571.
21 Tiesset, H, Bernard, H, Bartke, N, et al. (2011) (n-3) Long-chain PUFA differentially affect resistance to Pseudomonas aeruginosa infection of male and female cftr − / − mice. J Nutr 141, 11011107.
22 Lee, SE, Lim, JW, Kim, JM, et al. (2014) Anti-inflammatory mechanism of polyunsaturated fatty acids in Helicobacter pylori-infected gastric epithelial cells. Mediators Inflamm 2014, 128919.
23 Naqvi, AZ, Hasturk, H, Mu, L, et al. (2014) Docosahexaenoic acid and periodontitis in adults: a randomized controlled trial. J Dent Res 93, 767773.
24 Oh, DY, Walenta, E, Akiyama, TE, et al. (2014) A Gpr120-selective agonist improves insulin resistance and chronic inflammation in obese mice. Nat Med 20, 942947.
25 Cleland, LG, Caughey, GE, James, MJ, et al. (2006) Reduction of cardiovascular risk factors with long-term fish oil treatment in early rheumatoid arthritis. J Rheumatol 33, 19731979.
26 Larque, E, Gil-Sanchez, A, Prieto-Sanchez, MT, et al. (2012) Omega 3 fatty acids, gestation and pregnancy outcomes. Br J Nutr 107, Suppl. 2, S77S84.
27 Schwerbrock, NM, Karlsson, EA, Shi, Q, et al. (2009) Fish oil-fed mice have impaired resistance to influenza infection. J Nutr 139, 15881594.
28 Morita, M, Kuba, K, Ichikawa, A, et al. (2013) The lipid mediator protectin D1 inhibits influenza virus replication and improves severe influenza. Cell 153, 112125.
29 Hekmatdoost, A, Wu, X, Morampudi, V, et al. (2013) Dietary oils modify the host immune response and colonic tissue damage following Citrobacter rodentium infection in mice. Am J Physiol Gastrointest Liver Physiol 304, G917G928.
30 Calder, PC (2013) Omega-3 polyunsaturated fatty acids and inflammatory processes: nutrition or pharmacology? Br J Clin Pharmacol 75, 645662.
31 Lotz, S, Aga, E, Wilde, I, et al. (2004) Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2. J Leukoc Biol 75, 467477.
32 Maschmeyer, G & Braveny, I (2000) Review of the incidence and prognosis of Pseudomonas aeruginosa infections in cancer patients in the 1990s. Eur J Clin Microbiol Infect Dis 19, 915925.
33 Bonfield, TL, Konstan, MW & Berger, M (1999) Altered respiratory epithelial cell cytokine production in cystic fibrosis. J Allergy Clin Immunol 104, 7278.
34 Chmiel, JF & Davis, PB (2003) State of the art: why do the lungs of patients with cystic fibrosis become infected and why can't they clear the infection? Respir Res 4, 8.
35 Freedman, SD, Katz, MH, Parker, EM, et al. (1999) A membrane lipid imbalance plays a role in the phenotypic expression of cystic fibrosis in cftr ( − / − ) mice. Proc Natl Acad Sci U S A 96, 1399514000.
36 Dang, PM, Elbim, C, Marie, JC, et al. (2006) Anti-inflammatory effect of interleukin-10 on human neutrophil respiratory burst involves inhibition of GM-CSF-induced p47PHOX phosphorylation through a decrease in ERK1/2 activity. FASEB J 20, 15041506.
37 Cassatella, MA, Meda, L, Bonora, S, et al. (1993) Interleukin 10 (IL-10) inhibits the release of proinflammatory cytokines from human polymorphonuclear leukocytes. Evidence for an autocrine role of tumor necrosis factor and IL-1β in mediating the production of IL-8 triggered by lipopolysaccharide. J Exp Med 178, 22072211.
38 Chmiel, JF, Konstan, MW, Saadane, A, et al. (2002) Prolonged inflammatory response to acute Pseudomonas challenge in interleukin-10 knockout mice. Am J Respir Crit Care Med 165, 11761181.
39 Taggart, C, Coakley, RJ, Greally, P, et al. (2000) Increased elastase release by CF neutrophils is mediated by tumor necrosis factor-alpha and interleukin-8. Am J Physiol Lung Cell Mol Physiol 278, L33L41.
40 Ghosh, S, DeCoffe, D, Brown, K, et al. (2013) Fish oil attenuates omega-6 polyunsaturated fatty acid-induced dysbiosis and infectious colitis but impairs LPS dephosphorylation activity causing sepsis. PLOS ONE 8, e55468.
41 Murray, J, Barbara, JA, Dunkley, SA, et al. (1997) Regulation of neutrophil apoptosis by tumor necrosis factor-α: requirement for TNFR55 and TNFR75 for induction of apoptosis in vitro . Blood 90, 27722783.
42 Weber, N (1985) Metabolism of orally administered rac-1-O-[1′-14C]dodecylglycerol and nutritional effects of dietary rac-1-O-dodecylglycerol in mice. J Lipid Res 26, 14121420.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *



Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed