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    Lou-Bonafonte, José Gabás-Rivera, Clara Navarro, María and Osada, Jesús 2015. PON1 and Mediterranean Diet. Nutrients, Vol. 7, Issue. 6, p. 4068.

    Navarro, M.A. and Osada, J. 2015. El vino tinto es algo más que una bebida alcohólica. Revista Clínica Española, Vol. 215, Issue. 9, p. 503.

    Oliván, Sara Martínez-Beamonte, Roberto Calvo, Ana C. Surra, Joaquín C. Manzano, Raquel Arnal, Carmen Osta, Rosario and Osada, Jesús 2014. Extra virgin olive oil intake delays the development of amyotrophic lateral sclerosis associated with reduced reticulum stress and autophagy in muscle of SOD1G93A mice. The Journal of Nutritional Biochemistry, Vol. 25, Issue. 8, p. 885.


In comparison with palm oil, dietary nut supplementation delays the progression of atherosclerotic lesions in female apoE-deficient mice

  • Joaquín C. Surra (a1) (a2), Cristina Barranquero (a2) (a3), María P. Torcal (a3), Israel Orman (a4), Jose C. Segovia (a4), Natalia Guillén (a2) (a3), María A. Navarro (a2) (a3), Carmen Arnal (a2) (a5) and Jesús Osada (a2) (a3)
  • DOI:
  • Published online: 28 January 2012

Epidemiological studies have demonstrated the benefits of nut consumption on cardiovascular risk factors and CHD, attributed to their fatty acid profile, rich in unsaturated fatty acids, and also to other nutrients. The effect of nuts on atherosclerotic lesions was studied in female and male apoE-knockout mice fed a diet supplemented with 3 % (w/w) mixed nuts (mix: almonds, hazelnuts and walnuts in a proportion of 0·25:0·25:0·50, respectively), and compared with mice receiving an isoenergetic diet of similar fat content provided as palm oil. After 12 weeks, plasma lipid parameters and aortic lesions were measured. Males receiving nuts had lower plasma cholesterol than the palm oil group, and both sex groups had lower plasma non-HDL-cholesterol and lower content of reactive oxygen species in LDL than mice receiving the palm oil diet, the latter decrease being more pronounced in females than in males. Females consuming the nut diet showed a smaller aortic lesion area than those consuming palm oil, whereas no differences were observed in males. In females, hepatic paraoxonase 2 (Pon2) mRNA increased, and no change was observed in prenylcysteine oxidase 1 (Pcyox1) expression after the consumption of the nut-containing diet. In addition, aortic atherosclerotic lesions correlated directly with total plasma cholesterol and inversely with hepatic Pon2 expression. The results suggest that the beneficial effect of nut intake in female apoE-deficient mice may be attributed to reduced non-HDL-cholesterol levels and enhanced PON2 antioxidant activity.

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*Corresponding author: Dr J. Osada, fax +34 976 761 612, email
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