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The influence of different combinations of γ-linolenic acid, stearidonic acid and EPA on immune function in healthy young male subjects

  • Elizabeth A. Miles (a1), Tapati Banerjee (a1), Maaike M. B. W. Dooper (a2), Laura M'Rabet (a2), Yvo M. F. Graus (a2) and Philip C. Calder (a1)...
Abstract

To determine the effects of EPA, stearidonic acid (STA) or γ-linolenic acid (GLA) on immune outcomes, healthy male subjects consumed one of seven oil blends for 12 weeks. EPA consumption increased the EPA content of peripheral blood mononuclear cells (PBMC). Consumption of GLA (2·0 g/d) in the absence of STA or EPA increased di-homo-GLA content in PBMC. Neither STA nor its derivative 20:4n-3 appeared in PBMC when STA (<1·0 g/d) was consumed. However, STA (1·0 g/d), in combination with GLA (0·9 g/d), increased the proportion of EPA in PBMC. None of the treatments altered neutrophil or monocyte phagocytosis or respiratory burst, production of inflammatory cytokines by monocytes, T lymphocyte proliferation or the delayed-type hypersensitivity response. Production of cytokines by T lymphocytes increased in all groups, with no differences among them. The proportion of lymphocytes that were natural killer cells decreased significantly in subjects receiving 2·0 g EPA or GLA/d. There were no other effects on lymphocyte sub-populations. Plasma IgE concentration decreased in most groups, but not in the control group. Plasma IgG2 concentration increased in the EPA group. Thus, EPA or GLA at a dose of 2·0 g/d have little effect on key functions of neutrophils, monocytes and T lymphocytes, although at this dose these fatty acids decrease the number of natural killer cells. At this dose EPA increases IgG2 concentrations. STA can increase immune cell EPA status, but at 1·0 g/d does not affect human immune function.

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Corresponding author
*Corresponding author: Dr Elizabeth A. Miles, fax + 44 23 8059 4383, email eam@soton.ac.uk
References
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Blok WL, Deslypere J-P, Demacker PNM, van der Ven-Jongekrijg J, Hectors MPC, van der Meer JMW & Katan MB (1997) Pro- and anti-inflammatory cytokines in healthy volunteers fed various doses of fish oil for 1 year. Eur J Clin Invest 27, 10031008.
British Nutrition Foundation (1992) Report of the Task Force on Unsaturated Fatty Acids: Nutritional and Physiological Significance. London: Chapman & Hall.
British Nutrition Foundation (1999) Briefing Paper: n-3 Fatty Acids and Health. London: British Nutrition Foundation.
Burdge GC, Jones AE & Wootton SA (2002) Eicosapentaenoic and docosapentaenoic acids are the principal products of α-linolenic acid metabolism in young men. Br J Nutr 88, 355363.
Burdge GC & Wootton SA (2002) Conversion of α-linolenic acid to eicosapentaenoic, docosapentaenoic and docosahexaenoic acids in young women. Br J Nutr 88, 411420.
Calder PC (2001 a) Polyunsaturated fatty acids, inflammation and immunity. Lipids 36, 10071024.
Calder PC (2001 b) n-3 Polyunsaturated fatty acids, inflammation and immunity: pouring oil on troubled waters or another fishy tale? Nutr Res 21, 309341.
Calder PC (2002) Dietary modification of inflammation with lipids. Proc Nutr Soc 61, 345358.
Calder PC (2003) n-3 Polyunsaturated fatty acids and inflammation: from molecular biology to the clinic. Lipids 38, 342352.
Caughey GE, Mantzioris E, Gibson RA, Cleland LG & James MJ (1996) The effect on human tumour factor α and interleukin 1β production of diets enriched in n-3 fatty acids from vegetable oil or fish oil. Am J Clin Nutr 63, 116122.
De Luca P, Rossetti RG, Alavian C, Karim P & Zurier RB (1999) Effects of gammalinolenic acid on interleukin-1 beta and tumor necrosis factor-alpha secretion by stimulated human peripheral blood monocytes: studies in vitro and in vivo. J Invest Med 47, 246250.
Gibney MJ & Hunter B (1993) The effects of short term and long term supplementation with fish oil on the incorporation of n-3 polyunsaturated fatty acids into cells of the immune system in healthy volunteers. Eur J Clin Nutr 47, 255259.
Healy DA, Wallace FA, Miles EA, Calder PC & Newsholme P (2000) Effect of low to moderate amounts of dietary fish oil on neutrophil lipid composition and function. Lipids 35, 763768.
Huang YS, Smith RS, Redden PR, Cantrill RC & Horrobin DF (1991) Modification of liver fatty-acid metabolism in mice by n-3 and n-6 delta-6-desaturase substrates and products. Biochim Biophys Acta 1082, 319327.
Ishihara K, Komatsu W, Saito H & Shinohara K (2002) Comparison of the effects of dietary alpha-linolenic, stearidonic, and eicosapentaenoic acids on production of inflammatory mediators in mice. Lipids 37, 481486.
James MJ, Ursin VM & Cleland LG (2003) Metabolism of stearidonic acid in human subjects: comparison with the metabolism of other n-3 fatty acids. Am J Clin Nutr 77, 11401145.
Johnson MM, Swan DD, Surette ME, Stegner J, Chilton T, Fonteh AN & Chilton FH (1997) Dietary supplementation with gamma-linolenic acid alters fatty acid content and eicosanoid production in healthy humans. J Nutr 127, 14351444.
Kelley DS, Branch LB, Love JE, Taylor PC, Rivera YM & Iacono JM (1991) Dietary alpha-linolenic acid and immunocompetance in humans. Am J Clin Nutr 53, 4046.
Kew K, Banerjee T, Minihane AM, Finnegan YE, Muggli R, Albers R, Williams CR & Calder PC (2003) Lack of effect of foods enriched with plant- or marine-derived n-3 fatty acids on human immune function. Am J Clin Nutr 77, 12871295.
Mann DR, Akinbami MA, Gould KG & Ansari AA (2000) Seasonal variation in cytokine expression and cell-mediated immunity in male rhesus monkeys. Cell Immunol 200, 105115.
Meydani SN, Lichtenstein AH, Cornwall S, Meydani M, Goldin BR, Rasmussen H, Dinarello CA & Schaefer EJ (1993) Immunologic effects of national cholesterol education step-2 diets with and without fish-derived n-3 fatty acid enrichment. J Clin Invest 92, 105113.
Nelson RJ & Demas GE (1996) Seasonal changes in immune function. Q Rev Biol 71, 511548.
Rossetti RG, Seiler CM, De Luca P, Laposata M & Zurier RB (1997) Oral administration of unsaturated fatty acids: effects on human peripheral blood T lymphocyte proliferation. J Leukoc Biol 62, 438443.
Schmidt EB, Varming K, Moller JM, Bulow Pederson I, Madsen P & Dyerberg J (1996) No effect of very low dose n-3 fatty acids on monocyte function in healthy humans. Scand J Clin Invest 56, 8792.
Thies F, Miles EA, Nebe-von-Caron G, Powell JR, Hurst TL, Newsholme EA & Calder PC (2001 a) Influence of dietary supplementation with long-chain n-3 or n-6 polyunsaturated fatty acids on blood inflammatory cell populations and functions and on plasma soluble adhesion molecules in healthy humans. Lipids 36, 11831193.
Thies F, Nebe-von-Caron G, Powell JR, Yaqoob P, Newsholme EA & Calder PC (2001 b) Dietary supplementation with gamma linolenic acid or fish oil decreases T lymphocyte proliferation in healthy older humans. J Nutr 131, 19181927.
Thies F, Nebe-von-Caron G, Powell JR, Yaqoob P, Newsholme EA & Calder PC (2001 c) Dietary supplementation with eicosapentaenoic acid, but not with other long chain n 3 or n 6 polyunsaturated fatty acids, decreases natural killer cell activity in healthy subjects aged >55 y. Am J Clin Nutr 73, 539548.
Trebble T, Arden NK, Stroud MA, Wotton SA, Burge GC, Miles EA, Ballinger AB, Thompdon RL & Calder PC (2003 a) Inhibition of tumour necrosis factor-α and interleukin 6 production by mononuclear cells following dietary fish-oil supplementation in healthy men and response to antioxidant co-supplementation. Br J Nutr 90, 405412.
Trebble TM, Wootton SA, Miles EA, Mullee M, Arden NK, Ballinger AB, Stroud MA, Burdge GC & Calder PC (2003 b) Prostaglandin E2 production and T-cell function following dietary fish oil: response to antioxidant co-supplementation. Am J Clin Nutr 78, 376382.
Wallace FA, Miles EA & Calder PC (2003) Comparison of the effects of linseed oil and different doses of fish oil on mononuclear cell function in healthy humans. Br J Nutr 89, 679689.
Yamazaki K, Fujikawa M, Hamazaki T, Yano S & Shono T (1992) Comparison of the conversion rates of alpha-linolenic acid (18–3(n-3)) and stearidonic acid (18–4(n-3)) to longer polyunsaturated fatty-acids in rats. Biochim Biophys Acta 1123, 1826.
Yaqoob P, Pala HS, Cortina-Borja M, Newsholme EA & Calder PC (2000) Encapsulated fish oil enriched in α-tocopherol alters plasma phospholipid and mononuclear cell fatty acid compositions but not mononuclear cell functions. Eur J Clin Invest 30, 260274.
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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
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