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Influence of oat saponins on intestinal permeability in vitro and in vivo in the rat

  • Gunilla Önning (a1), Quan Wang (a2), Björn R Weström (a2), Nils-Georg Asp (a1) and Börje W Karlsson (a2)...

Abstract

The aim of the present study was to investigate whether oat saponins (avenacosides A and B) have any effect on the permeability of the rat intestine to actively and passively transported markers in vitro and in vivo. Intestinal segments were mounted in modified Ussing chambers, and thepassage of the different marker compounds from the mucosal to the serosal side was measured for 120 min. Avenacosides (1 mg/ml) gave a significantly higher passage of the macromolecule ovalbumin and there was a tendency to increased passage of [14C]D-mannitol and [15Cr]EDTA. On the other hand, the saponins did not affect the active transport of [3H]methyl glucose. When rats were given saponins (40 mg/kg body weight)together with markers by gastric intubation, the passage of [51Cr]EDTA into blood and urine was somewhat reduced. For the macromolecule bovine serum albumin, no evident effect on the passage was observed in the presence ofsaponins. Thus, in contrast to the in vitro results, the in vivo marker passage seemed to be unaffected or even reduced in the presence of avenacosides. The study shows that saponinscan affect the permeability of the rat intestine. However, this effect needs further investigation in vivo, especially regarding proteins.

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References

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Atkinson, H. A. C., Grogoriadou, F. & Miller, K. (1994). Enhancement of oral sensitisation to food allergens by the bioactive plant constituent Gypsophila saponin in the brown Norway rat. In Biochemical Biomarkers in Environmental Toxicology, Abstract Booklet. Cambridge: University of Cambridge.
Gee, J. M. & Johnson, I. T. (1988). Interactions between hemolytic saponins, bile acids andsmall intestinal mucosa in the rat. Journal of Nutrition 118, 13911397.
Gee, J. M., Price, K. R., Ridout, C. L., Wortley, G. M., Hurrell, R. F. & Johnson, I. T. (1993). Saponins of quinoa (Chenopodium quinoa): effects of processing on their abundance in quinoa products and their biological effects on intestinal mucosal tissue. Journal of the Science of Food and Agriculture 63, 201209.
Gestetner, B., Birk, Y. & Tencer, Y. (1968). Fate of ingested soybean saponins and the physiological aspect of their hemolytic activity. Journal of Agricultural and Food Chemistry 16, 10311035.
Hamilton, I., Rothwell, J., Archer, D. & Axon, A. T. R. (1987). Permeability of the rat small intestine to Carbohydrate probe molecules. Clinical Science 73, 189196.
Johnson, I. T., Gee, J. M., Price, K., Curl, C. & Fenwick, G. R. (1986). Influence of saponins on gut permeability and active nutrient transport in vitro. Journal of Nutrition 116, 22702277.
Lalitha, T., Vishwanatha, S. & Venkataraman, L. V. (1990). Oral toxicity of Modhuca butyracea Macb. saponins to albino rats. Indian Journal of Experimental Biology 28, 642647.
Mohr, W. (1953). Über die Qualitätsbeeinflussung von Hafererzeugnissen (III) Vorbericht üiber einen wasserlöslichen Bitterstoff aus Hafer (Influence on the quality of oat products (III). A preliminary report of a water soluble, bitter tasting compound in oats). Deutsche Lebensmittel-Rundschau 49, 127131.
Oakenfull, D. G. & Sidhu, G. S. (1990). Could saponins be a useful treatment for hypercholesterolaemia? European Journal of Clinical Nutrition 44, 7988.
Oleszek, W. (1990). Structural specificity of alfalfa (Medicago sativa) saponin haemolysis and its impacts on two haemolysis-based quantification methods Journal of the Science of Food and Agriculture 53, 477485.
Oleszek, W., Jurzysta, M., Ploszynski, M., Colquhoun, I. J., Price, K. R. & Fenwick, G. R. (1992). Zahnic acid tridesmoside and other dominant saponins from alfalfa (Medicago sativa L.) aerial parts. Journal of Agricultural and Food Chemistry 40, 191196.
Oleszek, W., Nowacka, J., Gee, J. M., Wortley, G. M. & Johnson, I. T. (1994). Effects of some purified alfalfa (Medicago sativa) saponins on transmural potential difference in mammalian small intestine Journal of the Science of Food and Agriculture 65, 3539.
Önning, G. & Asp, N. -G. (1993). Analysis of saponins in oat kernels. Food Chemistry 48, 301305.
Önning, G. & Asp, N. -G. (1995). Effect of oat saponins on plasma and liver lipids in gerbils and rats. British Journal of Nutrition 73, 275286.
Önning, G., Asp, N. -G. & Sivik, B. (1993). Saponin content in different oat varieties and in different fractions of oat grain. Food Chemistry 48, 251254.
Pantzar, N., Weström, B. R., Luts, A. & Lundin, S. (1993). Regional small-intestinal permeability in vitro to different-sized dextrans and proteins in the rat. Scandinavian Journal of Gastroenterology 28, 205211.
Price, K. R., Johnson, I. T. & Fenwick, G. R. (1987). The chemistry and biological significance of saponins in foods and feedingstuffs. CRC Critical Reviews in Food Science and Nutrition 26, 27135.
Rennard, S. I., Berg, R., Martin, G. R., Foidart, J. M. & Robey, P. G. (1980). Enzyme-linked immunoassay (ELISA) for connective tissue components. Analytical Biochemistry 104, 205214.
Sanderson, I. R. & Walker, W. A. (1993). Uptake and transport of macromolecules by the intestine: possible role in clinical disorders (an update). Gastroenterology 104, 622639.
Sidhu, G. S., Upson, B. & Malinow, M. R. (1987). Effects of soy saponins and tigogenin cellobioside on intestinal uptake of cholesterol, cholate and glucose. Nutrition Reports International 35, 615623.
Tschesche, R. & Lauven, P. (1971). Avenacosid B, ein zweites bisdesmosidisches Steroidsaponin aus Avena sativa (Avenacoside B, a second bisdesmosidic steroid saponin from Avena sativa). Chemische Berichtung 104, 35493555.
Tschesche, R., Tauscher, M., Fehlhaber, H. -W. & Wulff, G. (1969). Avenacosid A, ein bisdesmosidisches Steroidsaponin aus Avena sativa (Avenacoside A, a bisdesmosidic steroid saponin from Avena sativa). Chemische Berichtung 102, 20122082.
Tschesche, R. & Wiemann, W. (1977). Desglucoavenacosid-A and -B, biologisch aktive Nuatigenin glycoside (Desglucoavenacoside A and B, biologically active nuatigenin glycosides). Chemische Berichtung 110, 24162423.
Tschesche, R. & Wulff, G. (1973). Chemie und Biologie der Saponine. (Chemistry and biology of saponins). Progress in the Chemistry of Organic Natural Products 30, 461605.
Wall, M. E., Eddy, C. R., McClennan, M. L. & Klumpp, M. E. (1952). Detection and estimationof steroidal sapogenins in plant tissue. Analytical Chemistry 24, 13371341.
Wang, Q., Pantzar, N., Jeppsson, B., Weström, B. R. & Karlsson, B. W. (1994). Increased intestinal marker absorption due to regional permeability changes and decreased intestinal transitduring sepsis in the rat. Scandinavian Journal of Gastroenterology 28, 10011008.

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