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Menaquinone-4 in breast milk is derived from dietary phylloquinone

  • H. H. W. Thijssen (a1), M.-J. Drittij (a1), C. Vermeer (a2) and E. Schoffelen (a3)
Abstract

The effect of maternal phylloquinone supplementation on vitamin K in breast milk was studied to establish: (1) if phylloquinone is the source of menaquinone-4 in breast milk; (2) the dose–effect relationship between intake and obtainable levels. Four groups of lactating mothers with a full-term healthy infant participated and took oral phylloquinone supplements of 0·0 (n 8), 0·8 (n 8), 2·0 (n 8), and 4·0 (n 7) mg/d for 12 d, starting at day 4 post-partum. Milk samples were collected on days 4, 8, 16, and 19. Blood samples were collected on days 4 and 16. Vitamin K and vitamin E concentrations, the latter for reason of comparison, were assayed. Phylloquinone and menaquinone-4 were present in all milk samples: 5·84 (SD 2·31) AND 2·98 (sd 1·51) nmol/l (n 31) respectively, in colostrum (day 4 sample). A strong correlation between the vitamers was found (r 0·78, P<0·001). Breast-milk phylloquinone levels were raised in a dose-dependent manner: 4-, 12-, and 30-fold on day 16 for the 0·8, 2·0, and 4·0 mg group respectively. In addition, menaquinone-4 levels were higher: 2·5- (P<0·05) and 7-fold (P<0·001) in the 2·0 and 4·0 mg groups respectively. Plasma of supplemented subjects contained 3-, 5-, and 10-fold higher phylloquinone levels on day 16. Detectable menaquinone-4 was found in ten of thirty-one day 4 plasma samples. All day 16 plasma samples of the 4 mg supplemented group contained the vitamin. There was no correlation between the K-vitamers in plasma. Vitamin E and phylloquinone appear to differ in their distribution in breast milk, milk:plasma concentration ratios were ≤1 and 3–5 for vitamin E and phylloquinone respectively. The milk:plasma concentration ratio of menaquinone-4 was >10. In conclusion, dietary phylloquinone is a source of menaquinone-4 in breast milk. Phylloquinone supplementation to lactating mothers may be of benefit to the newborn infant, since both phylloquinone and menaquinone-4 are raised by supplementation.

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Corresponding author
*Corresponding author: Dr H.H.W. Thijssen, fax +31 43 388 41 49, email h.thijssen@farmaco.unimaas.nl
References
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Billeter M & Martius C (1960) Über die Umwandlung von Phylloquinon (Vitamin K1) in Vitamin K2(20) im Tierkörper (About the conversion of phylloquinone (vitamin K1) in to vitamin K2(20) in animal tissue). Biochemisches Zeitschrift 333, 430439.
Boersma ER, Offringa PJ, Muskiet FAJ, Chase WM & Simmons IJ (1991) Vitamin E, lipid fractions in, and fatty acid composition of colostrum, transitional milk, and mature milk: an international comparative study. American Journal of Clinical Nutrition 53, 11971204.
Booth SL & Suttie JW (1998) Dietary intake and adequacy of vitamin K. Journal of Nutrition 128, 785788.
Bosiletty S, Gupta JM, Graham GG, Salonikas C & Naidoo D (1998) Vitamin K in preterm breastmilk with maternal supplementation. Acta Paediatrica 87, 960962.
Buitenhuis HC, Soute BAM & Vermeer C (1990) Comparison of the vitamins K1, K2, and K3 as cofactors for the hepatic vitamin K-dependent carboxylase. Biochimica et Biophysica Acta 1034, 170175.
Chappell JE, Francis T & Clandinin MT (1985) Vitamin A and E content of human milk at early stages of lactation. Early Human Development 11, 157167.
Conly JM & Stein K (1992) The production of menaquinones (vitamin K2) by intestinal bacteria and their role in maintaining coagulation homeostasis. Progress in Food and Nutrition Science 16, 307343.
Cornelissen EAM, von Kries R, Loughnan P & Schubiger G (1997) Prevention of vitamin K deficiency bleeding: efficacy of different multiple oral dose schedules of vitamin K. European Journal of Pediatry 156, 126130.
Davidson RT, Foley AL, Engelke JA & Suttie JW (1998) Conversion of dietary phylloquinone to tissue menaquinone-4 in rats is not dependent on gut bacteria. Journal of Nutrition 128, 220223.
Dialameh GH, Taggart WV, Matshiner JT & Olson RE (1971) Isolation and characterization of menaquinone-4 as a product of menadione metabolism in chicks and rats. International Journal of Vitamin and Nutrition Research 41, 391400.
Fournier B, Sann L, Guillaumont M & Leclercq M (1987) Variations of phylloquinone concentration in human milk at various stages of lactation and in cow's milk at various seasons. American Journal of Clinical Nutrition 45, 551558.
Greer FR (1995) Vitamin K deficiency and hemorrhage in infancy. Clinical Perinatology 22, 759777.
Greer FR, Marshall SP, Foley AL & Suttie JW (1997) Improving the vitamin K status of breastfeeding infants with maternal vitamin K supplements. Pediatry 99, 8892.
Hachey DL, Thomas MR, Emken EA, Garza C, Brown-Booth L, Adlof RO & Klein PD (1987) Human lactation: maternal transfer of dietary triglycerides labeled with stable isotopes. Journal of Lipid Research 28, 11851192.
Hagstrom JN, Bovill EG, Soll RF, Davidson KW & Sadowski JA (1995) The pharmacokinetics and lipoprotein fraction distribution of intramuscular vs oral vitamin K1 supplementation in women of childbearing age: effects on hemostasis. Thrombosis and Haemostasis 74, 14861490.
Haroon Y, Bacon DS & Sadowski JA (1986) Liquid-chromatographic determination of vitamin K1 in plasma, with fluorometric detection. Clinical Chemistry 32, 19251929.
Harzer G, Haug M, Dieterich I & Gentner PR (1983) Changing patterns of human milk lipids in the course of lactation and during the day. American Journal of Clinical Nutrition 37, 612621.
Hiraike H, Kimura M & Itokawa Y (1988) Distribution of K vitamins (phylloquinone and menaquinones) in human placenta and maternal and umbilical cord plasma. American Journal of Obstetrics and Gynecology 158, 564569.
Indyk HE & Woollard DC (1997) Vitamin K in milk and infant formulas: determination and distribution of phylloquinone and menaquinone-4. Analyst 122, 465469.
Jie K-SG, Bots ML, Vermeer C, Witteman JCM & Grobbee DE (1995) Vitamin K intake and osteocalcin levels in women with and without aortic atherosclerosis: a population-based study. Atherosclerosis 116, 117123.
Kindberg C, Suttie JW, Uchida K, Hirauchi K & Nakao H (1987) Menaquinone production and utilization in germ-free rats after inoculation with specific organisms. Journal of Nutrition 117, 10321035.
King JC (2000) Physiology of pregnancy and nutrient metabolism. American Journal of Clinical Nutrition 71S, 218S225S.
Kohlmeier M, Salomon A, Saupe J & Shearer M (1996) Transport of vitamin K to bone in humans. Journal of Nutrition 126, 1192S1196S.
Meydan M (1995) Vitamin E. Lancet 345, 170175.
Neville MC, Keller R, Seacat J, Lutes V, Neifert M, Casey C, Allen J & Archer P (1988) Studies in human lactation: milk volumes in lactating women during the onset of lactation and full lactation. American Journal of Clinical Nutrition 48, 13751386.
Ronden JE (1998) Absorption, tissue distribution and bioactivity of vitamin K and related compounds in the rat. PhD Thesis, University of Maastricht.
Ronden JE, Drittij-Reijnders MJ, Vermeer C & Thijssen HHW (1998) Intestinal flora is not an intermediate in the phylloquinone–menaquinone-4 conversion in the rat. Biochimica et Biophysica Acta 1379, 6975.
Ramotar K, Conly JM, Chubb H & Louie TJ (1984) Production of menaquinones by intestinal anaerobes. Journal of Infectious Disease 150, 213218.
Shearer MJ (1992) Vitamin K metabolism and nutriture. Blood Reviews 6, 92104.
Sutor AH, Von Kries R, Cornelissen EAM & McNinh AW (1999) Vitamin K deficiency bleeding (VKDB) in infancy. Thrombosis and Haemostasis 81, 456461.
Suttie JW (1995) The importance of menaquinones in human nutrition. Annual Reviews of Nutrition 15, 399417.
The Netherlands Nutrition Centre (1998) Dutch Food Intake in 1997–1998. Den Haag: Voedingscentrum.
Thijssen HHW & Drittij-Reijnders MJ (1994) Vitamin K distribution in rat tissue: dietary phylloquinone is a source of tissue menaquinone-4. British Journal of Nutrition 72, 415425.
Thijssen HHW & Drittij-Reijnders MJ (1996) Vitamin K status in human tissues: tissue-specific accumulation of phylloquinone and menaquinone-4. British Journal of Nutrition 75, 121127.
Thijssen HHW, Drittij-Reijnders MJ & Fischer MAJG (1996) Phylloquinone and menaquinone-4 distribution in rats: synthesis rather than uptake determines menaquinone-4 organ concentrations. Journal of Nutrition 126, 537543.
Trumbo P, Yates AA, Schlicker S & Poos M (2001) Dietary reference intakes: Vitamin A, vitamin K, arsenic, boron, copper, iodine, iron, manganese, molybdenum, nickel, silicon, vanadium and zinc. Journal of the American Dietetic Association 101, 294301.
Will BH, Usui Y & Suttie JW (1992) Comparative metabolism and requirement of vitamin K in chicks and rats. Journal of Nutrition 122, 23542360.
Von Kries R, Shearer M, McCarthy PT, Haug M, Harzer G & Gobel U (1987) Vitamin K1 content of maternal milk: influence of the stage of lactation, lipid composition, and vitamin K1 supplements given to the mother. Pediatric Research 22, 513517.
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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
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