Skip to main content
×
Home
    • Aa
    • Aa
  • Access
  • Cited by 4
  • Cited by
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Kiec-Wilk, Beata Sliwa, Agnieszka Mikolajczyk, Magdalena Malecki, Maciej T. and Mathers, John C. 2011. The CpG Island Methylation Regulated Expression of Endothelial Proangiogenic Genes in Response to β-Carotene and Arachidonic Acid. Nutrition and Cancer, Vol. 63, Issue. 7, p. 1053.


    Campión, Javier Milagro, Fermin and Martínez, J. Alfredo 2010. Genes and Obesity.


    Coppedè, Fabio Grossi, Enzo Lopomo, Angela Spisni, Roberto Buscema, Massimo and Migliore, Lucia 2015. Application of artificial neural networks to link genetic and environmental factors to DNA methylation in colorectal cancer. Epigenomics, Vol. 7, Issue. 2, p. 175.


    Luo, Xiang Huang, Rong Sun, Hongru Liu, Yupeng Bi, Haoran Li, Jing Yu, Hongyuan Sun, Jiamei Lin, Shangqun Cui, Binbin and Zhao, Yashuang 2016. Methylation of a panel of genes in peripheral blood leukocytes is associated with colorectal cancer. Scientific Reports, Vol. 6, p. 29922.


    ×

Methylation status of CpG islands in the promoter region of genes differentially expressed in colonic mucosa from adenoma patients and controls in response to altered vegetable intake

  • Simone G. J. van Breda (a1), Joost H. M. van Delft (a1), Leopold G. J. B. Engels (a2), Jos C. S. Kleinjans (a1) and John C. Mathers (a3)
  • DOI: http://dx.doi.org/10.1017/S0007114508083529
  • Published online: 25 September 2008
Abstract

Vegetables may protect against colorectal cancer (CRC) via changes in gene expression involved in anticarcinogenic mechanisms. There is considerable evidence that aberrant DNA methylation plays an important role in carcinogenesis. Furthermore, DNA methylation can be affected by dietary components. Therefore, in the present study, we investigated the DNA methylation status of CpG dinucleotides within the promoter region of the four genes protein kinase C β 1, ornithine decarboxylase 1, fos proto-oncogene and 5,10-methylenetetrahydrofolate reductase in the colon of female sporadic adenoma patients and healthy controls. These genes were chosen because their expression was modulated in response to altered vegetable intake, they are functionally relevant for CRC; they have CpG islands in their promoter region, and a methylation-specific restriction enzyme is available to permit quantitative assay. No significant differences in extent of methylation in colon DNA were detected for any of the four genes in both adenoma polyp patients and healthy controls after altering vegetable intake. Interestingly, before the intervention, ornithine decarboxylase 1 promoter methylation was lower in the colonic mucosa of the adenoma polyp patients when compared with healthy control subjects, which may explain the increased ornithine decarboxylase 1 activity in CRC reported in the literature. In conclusion, we found no evidence that changes in promoter methylation were responsible for differences in expression of four genes in the human colonic mucosa in response to altered vegetable intake. The mechanism(s) responsible for this altered gene expression and, indeed, potential effects on methylation of other genes remain to be determined.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Methylation status of CpG islands in the promoter region of genes differentially expressed in colonic mucosa from adenoma patients and controls in response to altered vegetable intake
      Your Kindle email address
      Available formats
      ×
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about sending content to Dropbox.

      Methylation status of CpG islands in the promoter region of genes differentially expressed in colonic mucosa from adenoma patients and controls in response to altered vegetable intake
      Available formats
      ×
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about sending content to Google Drive.

      Methylation status of CpG islands in the promoter region of genes differentially expressed in colonic mucosa from adenoma patients and controls in response to altered vegetable intake
      Available formats
      ×
Copyright
Corresponding author
*Corresponding author: Dr Simone G. J. van Breda, fax +31 43 3884146, email s.vanbreda@grat.unimaas.nl
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

1KA Steinmetz & JD Potter (1996) Vegetables, fruit, and cancer prevention: a review. J Am Diet Assoc 96, 10271039.

3AP Feinberg , R Ohlsson & S Henikoff (2006) The epigenetic progenitor origin of human cancer. Nat Rev Genet 7, 2133.

4AM Jubb , SM Bell & P Quirke (2001) Methylation and colorectal cancer. J Pathol 195, 111134.

5EE Cameron , KE Bachman , S Myohanen , (1999) Synergy of demethylation and histone deacetylase inhibition in the re-expression of genes silenced in cancer. Nat Genet 21, 103107.

6SG van Breda , E van Agen , LG Engels , (2004) Altered vegetable intake affects pivotal carcinogenesis pathways in colon mucosa from adenoma patients and controls. Carcinogenesis 25, 22072216.

8AM Raizis , F Schmitt & JP Jost (1995) A bisulfite method of 5-methylcytosine mapping that minimizes template degradation. Anal Biochem 226, 161166.

9RJ Klose & AP Bird (2006) Genomic DNA methylation: the mark and its mediators. Trends Biochem Sci 31, 8997.

10JC Mathers (2006) Candidate mechanisms for interactions between nutrients and genes. In Nutrient-gene Interactions in Cancer, pp. 1936 [S-W Choi and F Simonetta , editors]. Boca Raton, FL: Taylor & Francis.

11JK Christman , G Sheikhnejad , M Dizik , (1993) Reversibility of changes in nucleic acid methylation and gene expression induced in rat liver by severe dietary methyl deficiency. Carcinogenesis 14, 551557.

13ME Martinez , TG O'Brien , KE Fultz , (2003) Pronounced reduction in adenoma recurrence associated with aspirin use and a polymorphism in the ornithine decarboxylase gene. Proc Natl Acad Sci U S A 100, 78597864.

14L Alhonen-Hongisto , P Leinonen , R Sinervirta , (1987) Mouse and human ornithine decarboxylase genes. Methylation polymorphism and amplification. Biochem J 242, 205210.

15M Halmekyto , A Hirvonen , J Wahlfors , (1989) Methylation of human ornithine decarboxylase gene before transfection abolishes its transient expression in Chinese hamster ovary cells. Biochem Biophys Res Commun 162, 528534.

16J Wahlfors (1991) Certain changes in ornithine decarboxylase gene methylation accompany gene amplification. Biochem J 279, 435440.

17IC Lawrance , C Fiocchi & S Chakravarti (2001) Ulcerative colitis and Crohn's disease: distinctive gene expression profiles and novel susceptibility candidate genes. Hum Mol Genet 10, 445456.

18WS Cutfield , PL Hofman & M Mitchell , (2007) Could epigenetics play a role in the developmental origins of health and disease? Pediatr Res 61, 68R75R.

19R Feil (2006) Environmental and nutritional effects on the epigenetic regulation of genes. Mutat Res 600, 4657.

20JC Mathers (2005) Reversal of DNA hypomethylation by folic acid supplements: possible role in colorectal cancer prevention. Gut 54, 579581.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords: