Skip to main content
×
×
Home

Morphological and immune response alterations in the intestinal mucosa of the mouse after short periods on a low-magnesium diet

  • W. Zimowska (a1), J. P. Girardeau (a2), J. Kuryszko (a3), D. Bayle (a1), Y. Rayssiguier (a1) and A. Mazur (a1)...
Abstract

The importance of Mg for the immune function is well recognized; however, there is no information available about the effect of Mg intake on the modulation of local immune response in the intestine. Thus, in the present study the hypothesis that short periods of Mg deprivation can affect intestinal mucosa and local immune response was tested. For this purpose, OF1 female mice were fed a semipurified diet (1000 mg Mg/kg diet). For 3 d before immunization and 1 d after, half of the animals were fed a Mg-deficient diet (30 mg Mg/kg diet), three immunizations per os were performed every 3 weeks with Escherichia coli producing the CS31A capsule-like protein (1010or 2×109 bacteria per animal). Mice were killed 10 d after the last immunization. The level of specific anti CS31A immunoglobulin (Ig) G and IgA in the serum and secretory IgA in the intestinal secretions and faeces were measured by ELISA. The results indicated that administration of a high dose of immunogen with a low-Mg diet led to lower specific IgA levels in the intestinal mucus and serum. Administration of a low dose of immunogen with a low-Mg diet led to lower IgA and IgG levels in the serum and secretory IgA coproantibodies. To assess alterations of intestinal mucosa caused by a low-Mg diet for a short period, histological and scanning electron microscopy analyses were performed on samples from mice (not submitted to the vaccination protocol) after 3 d on the Mg-deficient diet. These analyses showed several alterations, suggesting perturbations in the growth of the intestinal mucosa. These changes were accompanied by modifications in the expression of several genes involved in cell growth and stress response. From this present work, it may be concluded that short periods of Mg deprivation can affect the intestinal mucosa and local immune response of the intestine.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Morphological and immune response alterations in the intestinal mucosa of the mouse after short periods on a low-magnesium diet
      Available formats
      ×
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Morphological and immune response alterations in the intestinal mucosa of the mouse after short periods on a low-magnesium diet
      Available formats
      ×
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Morphological and immune response alterations in the intestinal mucosa of the mouse after short periods on a low-magnesium diet
      Available formats
      ×
Copyright
Corresponding author
*Corresponding author:Dr Andrzej Mazur, fax +33 4 73 62 42 34, email mazur@clermont.inra.fr
References
Hide All
Alcock, NW & Shils, ME (1974) Serum immunoglobulin G in the magnesium-depleted rat. Proceedings of the Society for Experimental Biology and Medicine 145, 855858.
Artizzu, M & Messier, B (1971) Histological changes in the gastric mucosa of magnesium deficient rats. British Journal of Experimental Pathology 52, 7074.
BD Biosciences (2002) Atlas(tm) Arrays and Gene Lists. Atlas mouse 1.2 array cat. no. 78531. http:/clontech.com/atlas/genelists/7853-1_Mo12.txt
Bussière, FI, Gueux, E, Rock, E, Girardeau, J-P, Tridon, A, Mazur, A & Rayssiguier, Y (2002) Increased phagocytosis and production of reactive oxygen species of neutrophils during magnesium deficiency in rats and inhibition by high magnesium concentration. British Journal of Nutrition 87, 107113.
Chandra, RK (1997) Nutrition and the immune system: an introduction. American Journal of Clinical Nutrition 66, 460S463S.
Chin, AI-D, Shu, J, Shi, CS, Yao, Z, Kehrl, JH & Cheng, G (1999) TANK potentiates tumor necrosis factor receptor-associated factor-mediated c-Jun N-terminal kinase/stress-activated protein kinase activation through the germinal center kinase pathway. Molecular and Cellular Biology 19, 66656672.
Chomczynski, P & Sacchi, N (1987) Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162, 156159.
Der Vartanian, M, Girardeau, JP, Martin, C, Rousset, E, Chavarot, M, Laude, H & Contrepois, M (1997) An Escherichia coli CS31A fibrillum chimera capable of inducing memory antibodies in outbred mice following booster immunization with the entero-pathogenic coronavirus transmissible gastroenteritis virus. Vaccine 15, 111120.
Durlach, J (1988) Magnesium in Clinical Practice [Durlach, J, editor]. London: John Libbey.
Elin, RJ (1975) The effect of magnesium deficiency in mice on serum immunoglobulin concentrations and antibody plaque-forming cells. Proceedings of the Society for Experimental Biology and Medicine 148, 620624.
Externest, D, Meckelein, B, Schmidt, MA & Frey, A (2000) Correlations between antibody immune responses at different mucosal effector sites are controlled by antigen type and dosage. Infection and Immunity 68, 38303839.
Galan, P, Preziosi, P, Durlach, V, Valeix, P, Ribas, L, Bouzid, D, Favier, A & Hercberg, S (1997) Dietary magnesium intake in a French adult population. Magnesium Research 10, 321328.
Galland, L (1988) Magnesium and immune function: an overview. Magnesium 7, 290299.
Guinee, PA, Jansen, WH & Agterberg, CM (1976) Detection of the K99 antigen by means of agglutination and immunoelectrophoresis in Escherichia coli isolates from calves and its correlation with entertoxigenicity. Infection and Immunity 13, 13691377.
Horne, MC, Donaldson, KL, Goolsby, GL, Tran, D, Mulheisen, M, Hell, JW & Wahl, AF (1997) Cyclin G2 is up-regulated during growth inhibition and B cell antigen receptor-mediated cell cycle arrest. Journal of Biological Chemistry 272, 1265012661.
Kraeuter, SL & Schwartz, R (1980) Blood and mast cell histamine levels in magnesium-deficient rats. Journal of Nutrition 110, 851858.
Kubena, KS, Cohill, DT & McMurray, DN (1989) Effect of varying levels of magnesium during gestation and lactation on humoral immune response and tissue minerals in rats. Annals of Nutrition and Metabolism 33, 714.
Kurantsin-Mills, BJ, Cassidy, MM, Stafford, RE & Weglicki, WB (1997) Marked alterations in circulating inflammatory cells during cardiomyopathy development in a magnesium-deficient rat model. British Journal of Nutrition 78, 845855.
Larvor, P (1980) Magnesium, humoral immunity, and allergy. In Magnesium in Health and Disease Proceedings of the 2nd International Symposium on Magnesium, pp. 201224 [Cantin, M and Seelig, MS, editors]. New York: SP Medical and Scientific Books.
McCoy, JH & Kenney, MA (1975) Depressed immune response in the magnesium-deficient rat. Journal of Nutrition 105, 791797.
McCoy, JH & Kenney, MA (1992) Magnesium and immune function: recent findings. Magnesium Research 5, 281293.
Macpherson, AJ, Hunziker, L, McCoy, K & Lamarre, A (2001) IgA responses in the intestinal mucosa against pathogenic and non-pathogenic microorganisms. Microbes in Infections 3, 10211035.
Malpuech-Brugère, C, Nowacki, W, Daveau, M, Gueux, E, Linard, C, Rock, E, Lebreton, J-P, Mazur, A & Rayssiguier, Y (2000) Inflammatory response following acute magnesium deficiency in the rat. Biochimica et Biophysica Acta 1501, 9198.
Malpuech-Brugère, C, Nowacki, W, Rock, E, Gueux, E, Mazur, A & Rayssiguier, Y (1999) Enhanced tumor necrosis factor-α production following endotoxin challenge in rats is an early event during magnesium deficiency. Biochimica et Biophysica Acta 1453, 3540.
Malpuech-Brugère, C, Rock, E, Astier, C, Nowacki, W, Mazur, A & Rayssiguier, Y (1998) Exacerbated immune stress response during experimental magnesium deficiency results from abnormal cell calcium homeostasis. Life Sciences 63, 18151822.
Mechin, MC, Rousset, E & Girardeau, JP (1996) Identification of surface-exposed linear B-cell epitopes of the nomfimbrial adhesin CS31A of Escherichia coli by using overlapping peptides and antipeptide antibodies. Infection and Immunity 64, 35553564.
Petrault, I, Zimowska, W, Mathieu, J, Bayle, D, Rock, E, Favier, A, Rayssiguier, Y & Mazur, A (2002) Changes in gene expression in rat thymocytes identified by cDNA array support the occurrence of oxidative stress in early magnesium deficiency. Biochimica et Biophysica Acta 1586, 9298.
Rayssiguier, Y, Bussiere, F, Gueux, E, Rock, E & Mazur, A (2001) Acute phase response in magnesium deficiency: possible relevance to atherosclerosis. In Advances in Magnesium Research: Nutrition and Health, pp. 277283 [Rayssiguier, Y, Mazur, A and Durlach, J, editors]. London: John Libbey.
Rayssiguier, Y, Larvor, P, Augusti, Y & Durlach, Y (1977) Rat serum proteins during magnesium deficiency. Annales de Biologie Animale Biochimie Biophysique 17, 147152.
Scrimshaw, NS & SanGiovanni, JP (1997) Synergism of nutrition, infection, and immunity: an overview. American Journal of Clinical Nutrition 66, 464S477S.
Simecka, JW (1998) Mucosal immunity of the gastrointestinal tract and oral tolerance. Advanced Drug Delivery Reviews 34, 235259.
Stammberger, P & Baczako, K (1999) Cytokeratin 19 expression in human gastrointestinal mucosa during human prenatal development and in gastrointestinal tumours: relation to cell proliferation. Cell and Tissue Research 298, 377381.
Weglicki, WB, Phillips, TM, Freedman, AM, Cassidy, MM & Dickens, BF (1992) Magnesium-deficiency elevates circulating levels of inflammatory cytokines and endothelin. Molecular and Cellular Biochemistry 110, 169173.
Windhauser, MM, Kappel, LC & Hegsted, M (1991) The humoral immune response in marginally and severely magnesium deficient rats. Journal of Nutritional Biochemistry 2, 541546.
Wolf, FI, Sgambato, A, Covacci, V, Faraglia, B, Torsello, A, Ardito, R & Cittadini, A (2001) Magnesium in the control cell of proliferation: a metabolic enhancer or a specific regulator? In Advances in Magnesium Research: Nutrition and Health, pp. 7381 [Rayssiguier, Y, Mazur, A and Durlach, J, editors]. London: John Libbey.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed