Skip to main content
×
×
Home

Phyto-oestrogen content of berries, and plasma concentrationsand urinary excretion of enterolactone after asingle strawberry-meal in human subjects

  • W. M. Mazur (a1), M. Uehara (a1), K. Wähälä (a1) and H. Adlercreutz (a1)
Abstract

Quantitative data on phyto-oestrogen, particularly lignan, content in edible plants are insufficient. We, therefore, measured isoflavonoids and lignans in nine edible berries using an isotope dilution gas chromatography–mass spectrometry method for foods and found substantial concentrations of the lignan secoisolariciresinol (1·39–37·18 mg/kg DM), low amounts of matairesinol (0–0·78 mg/kg DM) and no isoflavones. To determine pharmacokinetics and urinary excretion pattern of the mammalian lignan enterolactone derived from plant lignans, a study with human subjects was conducted. Five healthy women and two men consumed, after a 72 h period of a phyto-oestrogen-free regimen, a single strawberry-meal containing known amounts of plant lignans. Basal and post-meal blood and urine samples were collected at short intervals. The samples were analysed using time-resolved fluoroimmunoassay of enterolactone. The meal increased plasma concentration of enterolactone after 8–24 h and in urine in the 13–24 h and 25–36 h urine collections. High individual variability of the metabolic response was observed. Enterolactone excreted in the urine collected throughout the 48 h post-meal yielded on average 114 % of the plant lignans consumed. It is concluded that berries containing relatively high concentrations of plant lignans contribute to plasma and urinary levels of mammalian enterolactone in human subjects.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Phyto-oestrogen content of berries, and plasma concentrationsand urinary excretion of enterolactone after asingle strawberry-meal in human subjects
      Available formats
      ×
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about sending content to Dropbox.

      Phyto-oestrogen content of berries, and plasma concentrationsand urinary excretion of enterolactone after asingle strawberry-meal in human subjects
      Available formats
      ×
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about sending content to Google Drive.

      Phyto-oestrogen content of berries, and plasma concentrationsand urinary excretion of enterolactone after asingle strawberry-meal in human subjects
      Available formats
      ×
Copyright
Corresponding author
*Corresponding author: Professor Herman Adlercreutz, fax +358 9 61585633, email herman.adlercreutz@helsinki.fi
References
Hide All
Adlercreutz H (1998) Evolution, nutrition, intestinal microflora, and prevention of cancer: a hypothesis. Proceedings of the Society for Experimental Biology and Medicine 217, 241246.
Adlercreutz H, Fotsis T, Bannwart C, Hämäläinen E, Bloigu S and Ollus A (1986) Urinary estrogen profile determination in young Finnish vegetarian and omnivorous women. Journal of Steroid Biochemistry 24, 289296.
Adlercreutz H, Fotsis T, Bannwart C, Wähälä K, Brunow G and Hase T (1991) Isotope dilution gas chromatographic–mass spectrometric method for the determination of lignans and isoflavonoids in human urine, including identification of genistein. Clinica Chimica Acta 199, 263278.
Adlercreutz H, Fotsis T, Bannwart C, Wähälä K, Mäkelä T, Brunow G and Hase T (1986) Determination of urinary lignans and phytoestrogen metabolites, potential antiestrogens and anticarcinogens, in urine of women on various habitual diets. Journal of Steroid Biochemistry 25, 791797.
Adlercreutz H, Fotsis T, Heikkinen R, Dwyer JT, Woods M, Goldin BR and Gorbach SL (1982) Excretion of the lignans enterolactone and enterodiol and of equol in omnivorous and vegetarian women and in women with breast cancer. Lancet 2, 12951299.
Adlercreutz H, Fotsis T, Kurzer MS, Wähälä K, Mäkelä T and Hase T (1995) Isotope dilution gas chromatographic mass spectrometric method for the determination of unconjugated lignans and isoflavonoids in human feces, with preliminary results in omnivorous and vegetarian women. Analytical Biochemistry 225, 101108.
Adlercreutz H, Fotsis T, Lampe J, Wähälä K, Mäkelä T, Brunow G and Hase T (1993) Quantitative determination of lignans and isoflavonoids in plasma of omnivorous and vegetarian women by isotope dilution gas chromatography–mass spectrometry. Scandinavian Journal of Clinical and Laboratory Investigation 53, 518.
Adlercreutz H, Fotsis T, Watanabe S, Lampe J, Wähälä K, Mäkelä T and Hase T (1994) Determination of lignans and isoflavonoids in plasma by isotope dilution gas chromatography–mass spectrometry. Cancer Detection and Prevention 18, 259271.
Adlercreutz H, Höckerstedt K, Bannwart C, Bloigu S, Hämäläinen E, Fotsis T and Ollus A (1987) Effect of dietary components, including lignans and phytoestrogens, on enterohepatic circulation and liver metabolism of estrogens, and on sex hormone binding globulin (SHBG). Journal of Steroid Biochemistry 27, 11351144.
Adlercreutz H, Höckerstedt K, Bannwart C, Hämäläinen E, Fotsis T & Bloigu S (1988) Association between dietary fiber, urinary excretion of lignans and isoflavonic phytoestrogens, and plasma non-protein bound sex hormones in relation to breast cancer. In Progress in Cancer Research and Therapy, vol. 35: Hormones and Cancer 3, pp. 409412 [Bresciani F, King RJB, Lippman ME and Raynaud J-P, editors] New York, NY: Raven Press.
Adlercreutz H, Honjo H, Higashi A, Fotsis T, Hämäläinen E, Hasegawa T and Okada H (1991) Urinary excretion of lignans and isoflavonoid phytoestrogens in Japanese men and women consuming a traditional Japanese diet. American Journal of Clinical Nutrition 54, 10931100.
Adlercreutz H, Lapcik O, Hampl R, Wähälä K, Al-Maharik N, Wang G-J & Mikola H (1997) Immunoassay of phytoestrogens in human plasma. Symposium on Phytoestrogen Research Methods, Tucson, Arizona, USA. Abstr.
Adlercreutz H and Mazur W (1997) Phyto-oestrogens and western diseases. Annals of Medicine 29, 95120.
Adlercreutz H, Wang G-J, Lapcik O, Hampl R, Wähälä K, Mäkelä T, Lusa K, Talme M and Mikola H (1998) Time-resolved fluoroimmunoassay for plasma enterolactone. Analytical Biochemistry 265, 208215.
Adlercreutz H, Wang G-J, Uehara M, Lapcik O, Al-Maharik N, Mäkelä T, Mikola H, Hampl R & Wähälä K (1998 b) Immunoassays of phytoestrogens in human plasma. The Cost 916 Workshop on Phytoestrogens: Exposure, Bioavailability, Health Benefits and Safety Concerns. April 17–18, 1998, Doorwerth, The Netherlands, pp. 2328 [Bausch-Goldbolm S, Kardinaal S and Serra F, editors]. European Communities.
Axelson M and Setchell KDR (1981) The excretion of lignans in rats — evidence for an intestinal bacterial source for this new group of compounds. FEBS Letters 123, 337342.
Bannwart C, Adlercreutz H, Wähälä K, Brunow G & Hase T (1987) Identification of the isoflavonic phytoestrogens formononetin and dihydrodaidzein in human urine. In International Symposium on Applied Mass Spectrometry in the Health Sciences. Abstracts, p. 169. Barcelona: Fira de Barcelona, Palau de Congressos.
Borriello SP, Setchell KDR, Axelson M and Lawson AM (1985) Production and metabolism of lignans by the human faecal flora. Journal of Applied Bacteriology 58, 3743.
Brzezinski A, Adlercreutz H, Shaoul R, Rosler A, Shmueli A, Tanos V and Schenker JG (1997) Short-term effects of phytoestrogen-rich diet on postmenopausal women. Menopause — The Journal of the North American Menopause Society 4, 8994.
Cunnane SC, Hamadeh MJ, Liede AC, Thompson LU, Wolever TM and Jenkins DJ (1995) Nutritional attributes of traditional flaxseed in healthy young adults. American Journal of Clinical Nutrition 61, 6268.
Dewick PM (1993) Isoflavonoids. In The Flavonoids: Advances in Research Since 1986, pp. 117217 [Harborne JB, editor]. London: Chapman & Hall.
Jones AE, Price KR and Fenwick GR (1989) Development of high-performance liquid chromatographic method for the analysis of phytoestrogens. Journal of the Science of Food and Agriculture 46, 357364.
Karr SC, Lampe JW, Hutchins AM and Slavin JL (1997) Urinary isoflavonoid excretion in humans is dose dependent at low to moderate levels of soy-protein consumption. American Journal of Clinical Nutrition 66, 4651.
Kelly GE, Joannou GE, Reeder AY, Nelson C and Waring MA (1995) The variable metabolic response to dietary isoflavones in humans. Proceedings of the Society for Experimental Biology and Medicine 208, 4043.
Kurzer MS, Lampe JW, Martinit MC and Adlercreutz H (1995) Fecal lignan and isoflavonoid excretion in premenopausal women consuming flaxseed powder. Cancer Epidemiology, Biomarkers and Prevention 4, 353358.
Lampe JW, Martini MC, Kurzer MS, Adlercreutz H and Slavin JL (1994) Urinary lignan and isoflavonoid excretion in premenopausal women consuming flaxseed powder. American Journal of Clinical Nutrition 60, 122128.
Landström M, Zhang J-X, Hallmans G & Aring;man P, Bergh A, Damber JE, Mazur W, Wähälä K and Adlercreutz H (1998) Inhibitory effects of soy and rye diets on the development of Dunning R3327 prostate adenocarcinoma in rats. Prostate 36, 151161.
Mazur WM (1998) Phytoestrogens in food. In Baillière's Clinical Endocrinology and Metabolism: Phyto-oestrogens, vol. 12, no. 4, pp. 729742 [Adlercreutz H, editor]. London: Baillière Tindall.
Mazur WM and Adlercreutz H (1998) Naturally occurring oestrogens in food. Journal of Pure and Applied Chemistry 70, 17591776.
Mazur WM, Fotsis T, Wähälä K, Ojala S, Salakka A and Adlercreutz H (1996) Isotope dilution gas chromatographic–mass spectrometric method for the determination of isoflavonoids, coumestrol, and lignans in food samples. Analytical Biochemistry 233, 169180.
Morton MS, Wilcox G, Wahlqvist ML and Griffiths K (1994) Determination of lignans and isoflavonoids in human female plasma following dietary supplementation. Journal of Endocrinology 142, 251259.
Pettersson D, Aring;man P, Knudsen KEB, Lundin E, Zhang JX, Hallmans G, Harkonen H and Adlercreutz H (1996) Intake of rye bread by ileostomists increases ileal excretion of fiber polysaccharide components and organic acids but does not increase plasma or urine lignans and isoflavonoids. Journal of Nutrition 126, 15941600.
Price KR and Fenwick GR (1985) Naturally occurring oestrogens in foods — A review. Food Additions and Contaminants 2, 73106.
Reinli K and Block G (1996) Phytoestrogen content of foods — a compendium of literature values. Nutrition and Cancer 26, 123148.
Rowland IR, Mallett AK and Wise A (1985) The effect of diet on the mammalian gut flora and its metabolic activities. Critical Reviews in Toxicology 16, 31103.
Setchell KDR, Lawson AM, Borriello SPAdlercreutz H & Axelson M (1982) Formation of lignans by intestinal microflora. In Colonic Carcinogenesis: Falk Symposium 31, pp. 9397 [Malt RA, and Williamson RCN, editors]. Lancaster: MTP Press.
Shultz TD, Bonorden WR and Seaman WR (1991) Effect of short-term flaxseed consumption on lignan and sex hormone metabolism in men. Nutrition Research 11, 10891100.
Watanabe S, Yamaguchi M, Sobue T, Takahashi T, Miura T, Arai Y, Mazur W, Wähälä K and Adlercreutz H (1998) Pharmacokinetics of soybean isoflavones in plasma, urine and feces of men after ingestion of 60g baked soybean powder. Journal of Nutrition 128, 17101715.
Xu X, Harris KS, Wang HJ, Murphy PA and Hendrich S (1995) Bioavailability of soybean isoflavones depends upon gut microflora in women. Journal of Nutrition 125, 23072315.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 127 *
Loading metrics...

Abstract views

Total abstract views: 120 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 17th January 2018. This data will be updated every 24 hours.