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British Journal of Nutrition British Journal of Nutrition

Article contents

Plasma and tissue concentrations of α-tocopherol during vitamin E depletion in sheep

Published online by Cambridge University Press:  09 March 2007

J. M. Fry ,
G. M. Smith ,
M. C Mcgrath ,
E. J. Speijers  and
J. G. Allen
J. M. Fry
Affiliation:
Animal Health Division, Western Australian Department of Agriculture, Baron Hay-court, South Perth, Western Australia 6151, Australia
G. M. Smith
Affiliation:
Plant Industries Division, Western Australian Department of Agriculture, Baron Hay-court, South Perth, Western Australia 6151, Australia
M. C Mcgrath
Affiliation:
Animal Health Division, Western Australian Department of Agriculture, Baron Hay-court, South Perth, Western Australia 6151, Australia
E. J. Speijers
Affiliation:
Plant Industries Division, Western Australian Department of Agriculture, Baron Hay-court, South Perth, Western Australia 6151, Australia
J. G. Allen
Affiliation:
Animal Health Division, Western Australian Department of Agriculture, Baron Hay-court, South Perth, Western Australia 6151, Australia
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Abstract

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To determine the relationship between plasma and tissue α-tocopherol concentrations during vitamin E depletion, weaned lambs were placed on a vitamin E-deficient diet for 0, 1, 2, 4, 8 and 12 weeks. α-Tocopherol was measured in plasma, erythrocytes, liver, adrenal, adipose tissue, three different skeletal muscles and heart muscle. The α-tocopherol concentration in plasma fell at the same rate as the α-tocopherol concentration in skeletal muscles, heart muscle, adrenal and adipose tissue. The α-tocopherol concentration in liver and erythrocytes fell at a faster rate than that of plasma and all muscle tissues. There were significant correlations between α-tocopherol concentration in plasma and α-tocopherol concentrations in all the tissues measured. Different skeletal muscles had significantly different concentrations of α-tocopherol which may relate to their differing susceptibility to nutritional myopathy. The increase in malondialdehyde in oxidatively-stressed muscle tissue and the correlation with α-tocopherol concentration in most muscle tissues indicated that the muscles had reduced antioxidant capacity in vitro as a result of vitamin E depletion. It was concluded that during vitamin E depletion in sheep α-tocopherol concentration in plasma was a good index of vitamin E status under the experimental conditions employed.

Keywords

α-TocopherolVitamin ENutritional myopathySheep
Type
Vitamin Metabolism
Information
British Journal of Nutrition , Volume 69 , Issue 1 , January 1993 , pp. 225 - 232
Copyright
Copyright © The Nutrition Society 1993

References

Allen, J. G., Steele, P., Masters, H. G. & D'Antuono, M. F. (1986). A study of nutritional myopathy in weaner sheep. Australian Veterinary Journal 63, 8–13.CrossRefGoogle ScholarPubMed
Bieri, J. G. (1972). Kinetics of tissue α-tocopherol depletion and repletion. Annals of the New York Academy of Sciences 203, 181–191.CrossRefGoogle Scholar
Bieri, J. G., Pollard, C. J., Prange, I. & Dam, H. (1961). The determination of α-tocopherol in animal tissues by column chromatography. Acta Chemica Scandinavica 15, 783–790.CrossRefGoogle Scholar
Buttriss, J. L. & Diplock, A. T. (1988). The α-tocopherol and phospholipid fatty acid content of rat liver subcellular membranes in vitamin E and selenium deficiency. Biochimica et Biophysica Acta 963, 61–69.CrossRefGoogle ScholarPubMed
Gabbedy, B. J., Masters, H. & Boddington, E. B. (1977). White muscle disease of sheep and associated tissue selenium levels in Western Australia. Australian Veterinary Journal 53, 482–484.CrossRefGoogle ScholarPubMed
Hidiroglou, M. (1987). Vitamin E levels in sheep tissues at various times after a single oral administration of D-α-tocopherol acetate. International Journal of Vitamin and Nutrition Research 57, 381–384.Google ScholarPubMed
Hidiroglou, M. & Charmley, E. (1990). Response of plasma and tissue D-α-tocopherol in sheep to graded dietary levels of DL-α-tocopherol acetate. Research in Veterinary Science 49, 122–124.Google Scholar
Horwitt, M. K., Harvey, C. C., Dahni, C. H. & Searcy, M. T. (1972). Relationship between tocopherol and serum lipid levels for determination of nutritional adequacy. Annuls of the New York Academy of Sciences 203, 223–236.CrossRefGoogle ScholarPubMed
Jackson, M. J., Jones, D. A. & Edwards, R. H. T. (1983). Vitamin E and skeletal muscle. In Biology of Vitamin E. Ciba Foundation Symposium no. 101, pp. 224–234 [Porter, R. and Whelan, J., editors]. London: Pitman.Google Scholar
Kornbrust, D. J. & Mavis, R. D. (1980). Relative susceptibility of microsomes from lung, heart, liver, kidney, brain and testes to lipid peroxidation: correlation with vitamin E content. Lipids 15, 315–322.CrossRefGoogle ScholarPubMed
Machlin, L. J., Keating, J., Nelson, J., Brin, M., Filipski, R. & Miller, O. N. (1979). Availability of adipose tissue tocopherol in the guinea pig. Journal of Nutrition 109, 105–109.CrossRefGoogle ScholarPubMed
Mcmurray, C. H. & Blanchflower, W. J. (1979). Application of high performance liquid chromatographic fluorescence method for the rapid determination of α-tocopherol in the plasma of cattle and pigs and its comparison with direct fluorescence and high performance liquid chromatography ultraviolet detection methods. Journal of Chromatography 178, 525–531.CrossRefGoogle ScholarPubMed
Mcmurray, C. H., Rice, D. A. & Kennedy, S. (1983). Nutritional myopathy in cattle: from a clinical problem to experimental models for studying selenium, vitamin E and polyunsaturated fatty acid interactions. In Trace Elements in Animal Production and Veterinary Practice. Occasional Publication no. 7, pp. 61–13 [Suttle, N. F., Gunn, R.G., Allen, W. M., Linklater, K. A. and Wiener, G., editors]. Edinburgh: British Society of Animal Production.Google Scholar
Paynter, D. I., Halpin, C. G. & Caple, I. W. (1985). Measurement of blood glutathione peroxidase activity for assessment of selenium nutrition in livestock. Australian Standard Diagnostic Techniques, for Animal Diseases no. 45. Australian Agricultural Council Standing Committee on Agriculture. Melbourne: CSIRO.Google Scholar
Pudelkiewicz, W. J. & Mary, N. (1967). Some relationships between plasma, liver and excreta tocopherol in chicks fed graded levels of alpha-tocopherol acetate Journal of Nutrition 98, 303–306.Google Scholar
Rice, D. A., Blanchflower, W. J. & McMurray, C. H. (1985). The effects of moisture, propionic acid, sodium hydroxide and anaerobis on the stability of vitamin E in stored barley. Journal of Agricultural Science,CrossRefGoogle Scholar
Rice, D. A., Kennedy, S., Mcmurray, C. H. & Blanchflower, W. J. (1986). Differences in tissue concentrations of n-3 and n-6 fatty acids in vitamin E and selenium deficient cattle. Proceedings ofthe 6th International Conference on Production Disease in Farm Animals, pp. 229–232. Belfast: Veterinary Research Laboratory.Google Scholar
Rice, D. A. & McMurray, C. H. (1986). Use of sodium hydroxide treated selenium deficient barley to induce vitamin E and selenium deficiency in yearling cattle. Veterinary Record 118, 173–176.CrossRefGoogle ScholarPubMed
Richards, R. B., Passmore, I. K. & Dempsey, E. F. (1988). Skeletal muscle pathology in ovine congenital progressive muscular dystrophy. Acta Neuropathologica 77, 95–99.CrossRefGoogle ScholarPubMed
Salviati, G., Betto, R., Margeth, A., Novello, F. & Bonetti, E. (1980). Differential binding of vitamin E to sarcoplasmic reticulum from fast and slow muscles of the rabbit. Experientia 36, 1140–1141.CrossRefGoogle ScholarPubMed
Steele, P., Mckenzie, D. P., Skirrow, S., Peet, R. L. & Doncon, G. (1981). Selenium and tocopherol treatment of ovine weaner nutritional myopathy Trace Element Metabolism in Man and Animals (TEMA-4), pp. 210–213 [McHowell, J., Gawthorne, J. M. and White, J. L., editors]. Canberra: Australian Academy of Science.CrossRefGoogle Scholar
Steele, P., Peet, R. L., Skirrow, S., Hopkinson, W. & Masters, H. G. (1980). Low alpha-tocopherol levels in livers of weaner sheep with nutritional myopathy. Australian Veterinary Journal 56, 529–532.CrossRefGoogle ScholarPubMed
Thurnham, D. I., Davies, J. A., Crump, B. J., Situnayak, R. D. & Davis, M. (1986). The use of different lipids to express serum tocophero1:lipid ratios for the measurement of vitamin E status. Annals of Clinical Biochemistry 23, 514–520.CrossRefGoogle ScholarPubMed
White, N. A., McGavin, M. D. & Smith, J. E. (1978). Age related changes in percentage of fiber types and mean fiber diameters of the ovine quadriceps muscles. American Journal of Veterinary Research 39, 1297–1302.Google ScholarPubMed
Wiss, O., Bunnel, R. H. & Gloor, W. (1962). Absorption and distribution of vitamin E in the tissues. Vitamins and Hormones 20, 441–445.CrossRefGoogle Scholar
Wong, S. H. Y., Knight, J. A., Hopfer, S. M., Zaharia, O., Leach, C. N. Jr. & Sunderman, F. W. Jr. (1987). Lipoperoxides in plasma as measured by liquid-chromatographic separation of malondialdehyde-thiobarbituric acid adduct. Clinical Chemistry 33, 214–220.Google ScholarPubMed
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