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Polyphenol levels in human urine after intake of six different polyphenol-rich beverages

Published online by Cambridge University Press:  08 March 2007

Hideyuki Ito ,
Marie-Paule Gonthier ,
Claudine Manach ,
Christine Morand ,
Louise Mennen ,
Christian Rémésy  and
Augustin Scalbert
Hideyuki Ito
Affiliation:
Unité des Maladies Métaboliques et Micronutrients, INRA, 63122, Saint-Genès-Champanelle, France Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700-8530, Japan
Marie-Paule Gonthier
Affiliation:
Unité des Maladies Métaboliques et Micronutrients, INRA, 63122, Saint-Genès-Champanelle, France
Claudine Manach
Affiliation:
Unité des Maladies Métaboliques et Micronutrients, INRA, 63122, Saint-Genès-Champanelle, France
Christine Morand
Affiliation:
Unité des Maladies Métaboliques et Micronutrients, INRA, 63122, Saint-Genès-Champanelle, France
Louise Mennen
Affiliation:
UMR INSERM U557/INRA/CNAM, ISTNA-CNAM, Paris, France
Christian Rémésy
Affiliation:
Unité des Maladies Métaboliques et Micronutrients, INRA, 63122, Saint-Genès-Champanelle, France
Augustin Scalbert*
Affiliation:
Unité des Maladies Métaboliques et Micronutrients, INRA, 63122, Saint-Genès-Champanelle, France
*
*Corresponding author: Dr Augustin Scalbert, fax +33 4 73 62 46 38, email scalbert@clermont.inra.fr
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Abstract

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Dietary polyphenols are suggested to participate in the prevention of CVD and cancer. It is essential for epidemiological studies to be able to compare intake of the main dietary polyphenols in populations. The present paper describes a fast method suitable for the analysis of polyphenols in urine, selected as potential biomarkers of intake. This method is applied to the estimation of polyphenol recovery after ingestion of six different polyphenol-rich beverages. Fifteen polyphenols including mammalian lignans (enterodiol and enterolactone), several phenolic acids (chlorogenic, caffeic, m-coumaric, gallic, and 4-O-methylgallic acids), phloretin and various flavonoids (catechin, epicatechin, quercetin, isorhamnetin, kaempferol, hesperetin, and naringenin) were simultaneously quantified in human urine by HPLC coupled with electrospray ionisation mass-MS (HPLC-electrospray-tandem mass spectrometry) with a run time of 6 min per sample. The method has been validated with regard to linearity, precision, and accuracy in intra- and inter-day assays. It was applied to urine samples collected from nine volunteers in the 24 h following consumption of either green tea, a grape-skin extract, cocoa beverage, coffee, grapefruit juice or orange juice. Levels of urinary excretion suggest that chlorogenic acid, gallic acid, epicatechin, naringenin or hesperetin could be used as specific biomarkers to evaluate the consumption of coffee, wine, tea or cocoa, and citrus juices respectively.

Keywords

PolyphenolsFlavonoidsPhenolic acidsHigh-throughput analysisUrineBeverages
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 4 , October 2005 , pp. 500 - 509
Copyright
Copyright © The Nutrition Society 2005

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