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Preferential incorporation of trans, trans-conjugated linoleic acid isomers into the liver of suckling rats

Published online by Cambridge University Press:  09 March 2007

Lin Yang ,
Sai Ying Venus Yeung ,
Yu Huang ,
Han Qing Wang  and
Zhen-Yu Chen
Lin Yang
Affiliation:
State Key Laboratory of OXO Synthesis & Selective Oxidation, Lanzhou Institute of Chemical Physics, The Chinese Academy of Sciences, Lanzhou, The People's Republic of China Department of Biochemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, The People's Republic of China
Sai Ying Venus Yeung
Affiliation:
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, The People's Republic of China
Yu Huang
Affiliation:
Department of Physiology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, The People's Republic of China
Han Qing Wang
Affiliation:
State Key Laboratory of OXO Synthesis & Selective Oxidation, Lanzhou Institute of Chemical Physics, The Chinese Academy of Sciences, Lanzhou, The People's Republic of China
Zhen-Yu Chen*
Affiliation:
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, The People's Republic of China
*
*Corresponding author: Dr Zhen-Yu Chen, fax +852 2603 5123, email zhenyuchen@cuhk.edu.hk
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Abstract

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The present study was designed to compare the conjugated linoleic acid (CLA) isomeric distribution pattern in the liver of suckling rats in relation to those in the milk and maternal diet. Silver-ion HPLC was used to separate individual CLA isomers. It was found that the isomeric distribution pattern in the milk was very similar to that in the maternal dietary fat. However, the CLA isomeric distribution patterns in the liver phospholipids (PL) and triacylglycerols were different from those in the diet and milk. In the liver PL, total cis/trans isomers accounted for 63·6–63·9 % of total CLA, which was in contrast to the values of 88·1–89·1 % in the milk and diet. In the liver PL, total trans/trans isomers were 20·6–20·8 % of the total CLA isomers whereas they were only 2·6–3·7 % in the milk and diet. It is concluded that trans/trans-CLA were preferentially incorporated into the liver whereas for the incorporation of cis/trans-CLA there was partial discrimination.

Keywords

Conjugated linoleic acidsMilkNeonatePhospholipidsTriacylglycerols
Type
Research Article
Information
British Journal of Nutrition , Volume 87 , Issue 3 , March 2002 , pp. 253 - 260
Copyright
Copyright © The Nutrition Society 2002

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