Skip to main content
×
Home
    • Aa
    • Aa

Relationship between tissue lipid peroxidation and peroxidizability index after α-linolenic, eicosapentaenoic, or docosahexaenoic acid intake in rats

  • Morio Saito (a1) and Kazuhiro Kubo (a1) (a2)
Abstract

In a previous study, we found that the extent of dietary n-3 docosahexaenoic acid (DHA)-stimulated tissue lipid peroxidation was less than expected from the relative peroxidizability index of the total tissue lipids in rats with adequate vitamin E nutritional status. This suppression of lipid peroxidation was especially prominent in the liver. To elucidate whether this phenomenon was unique to DHA, we compared the peroxidation effects of n-3 α-linolenic acid (α-LN) and n-3 eicosapentaeonic acid (EPA) with those of DHA in rats. Either α-LN (8·6 % of total energy), EPA (8·2 %), or DHA (8·0 %) and one of two levels of dietary vitamin E (7·5 and 54 mg/kg diet) were fed to rats for 22 d. Levels of conjugated diene, chemiluminescence emission and thiobarbituric acid (TBA)-reactive substance in the liver, kidney, and testis were determined as indicators of lipid peroxidation. In rats fed the DHA diet deficient in vitamin E (7·5 m/g diet), TBA values in the liver, kidney, and testis correlated well with the tissues' relative peroxidizability indices. In rats fed the α-LN diet with an adequate level of vitamin E (54 m/g diet), a close association between relative peroxidizability indices and lipid peroxide levels was observed in all the tissues analysed. However, in rats fed either the EPA diet or the DHA diet with an adequate level of vitamin E, the extent of lipid peroxidation in each tissue was less than expected from the relative peroxidizability index. This suppression was particularly marked in the liver. We concluded that suppression of lipid peroxidation below the relative peroxidizability index was not unique to DHA, but was also seen with EPA, which has five double bonds, in rats with adequate vitamin E nutritional status, but not with α-LN, which has three double bonds.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Relationship between tissue lipid peroxidation and peroxidizability index after α-linolenic, eicosapentaenoic, or docosahexaenoic acid intake in rats
      Available formats
      ×
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about sending content to Dropbox.

      Relationship between tissue lipid peroxidation and peroxidizability index after α-linolenic, eicosapentaenoic, or docosahexaenoic acid intake in rats
      Available formats
      ×
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about sending content to Google Drive.

      Relationship between tissue lipid peroxidation and peroxidizability index after α-linolenic, eicosapentaenoic, or docosahexaenoic acid intake in rats
      Available formats
      ×
Copyright
Corresponding author
*Corresponding author: Dr Morio Saito, fax +81 3 3202 3278, email msaito@nih.go.jp
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

M Crawford , C Galli , F Visioli , S Renaud , AP Simopoulos & AA Spector (2000) Role of plant-derived omega-3 fatty acids in human nutrition. Annals of Nutrition and Metabolism 44, 263265.

CA Drevon (1992) Marine oils and their effects. Nutrition Reviews 50, 3845.

DB Duncan (1957) Multiple range tests for correlated and heteroscedastic means. Biometrics 13, 164176.

J Dyerberg (1986) Linolenate-derived polyunsaturated fatty acids and prevention of atherosclerosis. Nutrition Reviews 44, 125134.

BL Fletcher , CJ Dillard & AL Tappel (1973) Measurement of fluorescent lipid peroxidation products in biological systems and tissues. Analytical Biochemistry 52, 19.

SG Kaasgaard , G Holmer , CE Hoy , WA Behrens & JL Beare-Rogers (1992) Effect of dietary linseed oil and marine oil on lipid peroxidation in monkey liver in vivo and in vitro. Lipids 27, 740745.

Y Kobatake , F Hirahara , S Innami & E Nishide (1983) Dietary effect of n-3 type polyunsaturated fatty acids on serum and liver lipid levels in rats. Journal of Nutritional Science and Vitaminology 29, 1121.

K Kubo , M Saito , T Tadokoro & A Maekawa (1998) Dietary docosahexaenoic acid does not promote lipid peroxidation in rat tissue to the extent expected from peroxidizability index of the lipid. Bioscience, Biotechnology, and Biochemistry 62, 16981706.

A Meister (1992) On the antioxidant effects of ascorbic acid and glutathione. Biochemical Pharmacology 44, 19051915.

T Miyazawa , T Kaneda , C Takyu , A Yamaguchi & H Inaba (1981) Generation of singlet molecular oxygen in rat liver homogenate on adding autoxidized linseed oil. Agricultural and Biological Chemistry 45, 15971601.

K Mouri , H Ikesu , T Esaka & O Igarashi (1984) The influence of marine oil intake upon levels of lipids, α-tocopherol and lipid peroxidation in serum and liver of rats. Journal of Nutritional Science and Vitaminology 30, 307318.

PJ Nestel (1990) Effects of n-3 fatty acids on lipid metabolism. Annual Review of Nutrition 10, 149167.

H Ohkawa , N Ohishi & K Yagi (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry 95, 351358.

A Pietrangelo , R Grandi , A Tripodi , A Tomasi , D Ceccarelli , E Ventura & A Masini (1990) Lipid composition and fluidity of liver mitochondria, microsomes and plasma membrane of rats with chronic dietary iron overload. Biochemical Pharmacology 39, 123128.

S Reitman & S Frankel (1957) A Colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminase. American Journal of Clinical Pathology 28, 5663.

M Saito (1990) Polychlorinated biphenyls-induced lipid peroxidation as measured by thiobarbituric acid-reactive substances in liver subcellular fractions of rats. Biochimica et Biophysica Acta 1046, 301308.

M Saito , K Kubo & S Ikegami (1996) An assessment of docosahexaenoic acid (DHA) intake with special reference to lipid metabolism in rats. Journal of Nutritional Science and Vitaminology 42, 195207.

M Saito , K Nakatsugawa , A Oh-hashi , M Nishimuta & N Kodama (1992) Comparison of vitamin E levels in human plasma, red blood cells, and platelets following varying intakes of vitamin E. Journal of Clinical Biochemistry and Nutrition 12, 5968.

AL Tappel (1962) Vitamin E as the biological lipid antioxidant. Vitamins and Hormones 20, 493510.

F Visioli , C Colombo & C Galli (1998) Oxidation of individual fatty acids yields different profiles of oxidation markers. Biochemical and Biophysical Research Communications 245, 487489.

WW Wells , DP Xu & MP Washburn (1995) Glutathione: dehydroascorbate oxidoreductases. Methods in Enzymology 252, 3038.

BS Winkler , SM Orselli & TS Rex (1994) The redox couple between glutathione and ascorbic acid: a chemical and physiological perspective. Free Radical Biology and Medicine 17, 333349.

K Yagi (1976) A simple fluorometric assay for lipoperoxide in blood plasma. Biochemical Medicine 15, 212216.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 137 *
Loading metrics...

Abstract views

Total abstract views: 100 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 26th September 2017. This data will be updated every 24 hours.