Skip to main content Accessibility help
×
Home

A1-3 chromosomal translocations in Italian populations of the peach potato aphid Myzus persicae (Sulzer) not linked to esterase-based insecticide resistance

  • Marco Rivi (a1), Valentina Monti (a1), Emanuele Mazzoni (a2), Stefano Cassanelli (a1), Michela Panini (a2), Matteo Anaclerio (a2), Manuela Cigolini (a2), Bruna Corradetti (a3), Davide Bizzaro (a3), Mauro Mandrioli (a1) and Gian Carlo Manicardi (a1)...

Abstract

Esterase-based resistance in the peach-potato aphid, Myzus persicae (Sulzer), is generally due to one of two alternative amplified carboxylesterase genes, E4 or FE4 (fast E4). The E4 amplified form is distributed worldwide and it is correlated with a particular translocation between autosomes 1 and 3, whereas the FE4 form, which has hitherto not been found to be associated with chromosomal rearrangements, is typical of the Mediterranean regions. In this study, we present for the first time cytogenetic and molecular data on some M. persicae parthenogenetic lineages, which clearly show a chromosomal A1-3 translocation associated with esterase FE4 genes and unrelated to high levels of esterase-based resistance.

Copyright

Corresponding author

* Author for correspondence Phone: +39-0522-522059; Fax: +39-0522-522027 E-mail: giancarlo.manicardi@unimore.it

References

Hide All
Bass, C. & Field, L.M. (2011) Gene amplification and insecticide resistance. Pest Management Science 67, 886890.
Bizzaro, D., Manicardi, G.C. & Bianchi, U. (1996) Chromosomal localization of a highly repeated EcoRI DNA fragment in Megoura viciae (Homoptera, Aphididae) by nick translation and fluorescence in situ hybridization. Chromosome Research 4, 392396.
Bizzaro, D., Mazzoni, E., Barbolini, E., Giannini, S., Cassanelli, S., Pavesi, F., Cravedi, P. & Manicardi, G.C. (2005) Relationship among expression, amplification, and methylation of FE4 esterase genes in Italian populations of Myzus persicae (Sulzer) (Homoptera: Aphididae). Pesticide Biochemistry and Physiology 81, 5158.
Blackman, R.L. (1971) Chromosomal abnormalities in an anholocyclic biotype of Myzus persicae (Sulzer). Experientia 27, 704706.
Blackman, R.L. & Devonshire, A.L. (1978) Further studies on the genetics of the carboxylesterase regulatory system involved in resistance to organophosphorus insecticides in Myzus persicae (Sulzer). Pesticide Science 9, 517521.
Blackman, R.L. & Eastop, V.F. (2000) Aphids of the Worlds’ Crops. 2nd edn. John Wiley & Sons Ltd. Chicester, U.K.
Blackman, R.L., Takada, H. & Kawakami, K. (1978) Chromosomal rearrangement involved in insecticide resistance of Myzus persicae . Nature 271, 450452.
Blackman, R.L., Spence, J.M., Field, L.M. & Devonshire, A.L. (1995) Chromosomal location of the amplified esterase genes conferring resistance to insecticides in Myzus persicae (Homoptera: Aphididae). Heredity 75, 297302.
Blackman, R.L., Spence, J.M., Field, L.M. & Devonshire, A.L. (1999) Variation in the chromosomal distribution of amplified esterase (FE4) genes in Greek field populations of Myzus persicae (Sulzer). Heredity 82, 180186.
Criniti, A., Mazzoni, E., Cassanelli, S., Cravedi, P., Tondelli, A., Bizzaro, D. & Manicardi, G.C. (2008) Biochemical and molecular diagnosis of insecticide resistance conferred by esterase, MACE, kdr and super-kdr based mechanisms in Italian strains of the peach potato aphid, Myzus persicae (Sulzer). Pesticide Biochemistry and Physiology 90, 168174.
Devonshire, A.L. (1989) The role of electrophoresis in the biochemical detection of insecticide resistance. pp. 363374 in Loxdale, H.D. & den Hollander, J. (Eds) Electrophoretic Studies on Agricultural Pests. Clarendon Press, Oxford.
Devonshire, A.L., Field, L.M., Foster, S.P., Moores, G.D., Williamson, M.S. & Blackman, R.L. (1998) The evolution of insecticide resistance in the peach-potato aphid, Myzus persicae . Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences 353, 16771684.
Ffrench-Constant, R.H., Devonshire, A.L. & Clark, S.J. (1987) Differential rate of selection for resistance by carbamate, organophosphorus and combined pyrethroid and organophosphorus insecticides in Myzus persicae (Sulzer) (Hemiptera: Aphididae). Bulletin of Entomological Research 77, 227238.
Field, L.M. & Blackman, R.L. (2003) Insecticide resistance in the aphid Myzus persicae (Sulzer): chromosome location and epigenetic effects on esterase gene expression in clonal lineages. (Intraclonal genetic variation: ecological and evolutionary aspects.) Biological Journal of the Linnean Society 79, 107113.
Field, L.M. & Devonshire, A.L. (1998) Evidence that the E4 and FE4 esterase genes responsible for insecticide resistance in the aphid Myzus persicae (Sulzer) are part of a gene family. Biochemical Journal (London) 330, 169173.
Field, L.M. & Foster, S.P. (2002) Amplified esterase genes and their relationship with other insecticide resistance mechanisms in English field populations of the aphid, Myzus persicae (Sulzer). Pest Management Science 58, 889894.
Field, L.M., Williamson, M.S., Moores, G.D. & Devonshire, A.L. (1993) Cloning and analysis of the esterase genes conferring insecticide resistance in the potato-peach aphid, Myzus persicae . Biochemical Journal (London) 294, 569574.
Field, L.M., Crick, S.E. & Devonshire, A.L. (1996) Polymerase chain reaction-based identification of insecticide resistance genes and DNA methylation in the aphid Myzus persicae (Sulzer). Insect Molecular Biology 5, 197202.
Field, L.M., Blackman, R.L., TylerSmith, C. & Devonshire, A.L. (1999) Relationship between amount of esterase and gene copy number in insecticide-resistant Myzus persicae (Sulzer). Biochemical Journal (London) 339, 737742.
Foster, S.P., Denholm, I. & Devonshire, A.L. (2000) The ups and downs of insecticide resistance in peach-potato aphids (Myzus persicae) in the UK. Crop Protection 19, 873879.
Guillemaud, T., Brun, A., Anthony, N., Sauge, M.H., Boll, R., Delorme, R., Fournier, D., Lapchin, L. & Vanlerberghe Masutti, F. (2003) Incidence of insecticide resistance alleles in sexually-reproducing populations of the peach-potato aphid Myzus persicae (Hemiptera: Aphididae) from southern France. Bulletin of Entomological Research 93, 289297.
Karunaratne, S.H.P.P., Hemingway, J., Jayawardena, K.G.I., Dassanayaka, V. & Vaughan, A. (1995) Kinetic and molecular differences in the amplified and non-amplified esterases from insecticide-resistant and susceptible Culex quinquefasciatus mosquitoes. Journal of Biological Chemistry 270, 3112431128.
Kwon, D., Choi, B., Lee, S., Clark, J.M. & Lee, S. (2009) Characterization of carboxylesterase-mediated pirimicarb resistance in Myzus persicae . Pesticide Biochemistry and Physiology 93, 120126.
Livak, K.J. & Schmittgen, T.D. (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods (San Diego, California) 25, 402408.
Mazzoni, E. & Cravedi, P. (2002) Analysis of insecticide-resistant Myzus persicae (Sulzer) populations collected in Italian peach orchards. Pest Management Science 58, 975980.
Needham, P.H. & Sawicki, R.M. (1971) Diagnosis of resistance to organophosphorus insecticides in Myzus persicae (Sulz.). Nature 230, 125126.
Pfaffl, M.W. (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research 29, e45.
Rivi, M., Mazzoni, E., Criniti, A., Cassanelli, S., Bizzaro, D. & Manicardi, G.C. (2009) Relationship between chromosomal translocation and FE4 gene amplification in an Italian population of the peach-potato aphid Myzus persicae (Hemiptera: Aphididae). Redia 92, 229231.
Rivi, M., Monti, V., Mazzoni, E., Cassanelli, S., Panini, M., Bizzaro, D., Mandrioli, M. & Manicardi, G.C. (2012)Karyotype variations in Italian populations of the peach potato aphid Myzus persicae (Hemiptera: Aphididae). Bulletin of Entomological Research 102, 663671.
Takada, H. (1979) Estersae variation in Japanese populations of Myzus persicae (Sulzer) (Homoptera: Aphididae), with special reference to resistance to organophosphorous insecticides. Applied Entomology and Zoology 14, 245255.
Takada, H. (1986) Genotypic composition and insecticide resistance of Japanese populations of Myzus persicae (Sulzer) (Hom., Aphididae). Journal of Applied Entomology 102, 1938.

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed