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Deformed wing virus type a and b in managed honeybee colonies of Argentina

Published online by Cambridge University Press:  29 June 2020

C. Brasesco Open the ORCID record for C. Brasesco [Opens in a new window] ,
S. Quintana ,
V. Di Gerónimo ,
M. L. Genchi García ,
G. Sguazza ,
M. E. Bravi ,
L. Fargnoli ,
F. J. Reynaldi ,
M. Eguaras  and
M. Maggi
C. Brasesco*
Affiliation:
Centro de Investigación en Abejas Sociales, Laboratorio de Artrópodos, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas, Mar del Plata, Buenos Aires, Argentina
S. Quintana
Affiliation:
Centro de Investigación en Abejas Sociales, Laboratorio de Artrópodos, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas, Mar del Plata, Buenos Aires, Argentina Laboratorio de Biología Molecular, Instituto de Análisis Fares Taie, Mar del Plata, Argentina
V. Di Gerónimo
Affiliation:
Laboratorio de Biología Molecular, Instituto de Análisis Fares Taie, Mar del Plata, Argentina
M. L. Genchi García
Affiliation:
Laboratorio de Virología (LAVIR). Facultad de Ciencias Veterinarias. Universidad Nacional de La Plata, La Plata, Argentina Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC-PBA), Buenos Aires, Argentina
G. Sguazza
Affiliation:
Consejo Nacional de Investigaciones Científicas y Técnicas, Mar del Plata, Buenos Aires, Argentina
M. E. Bravi
Affiliation:
Consejo Nacional de Investigaciones Científicas y Técnicas, Mar del Plata, Buenos Aires, Argentina Laboratorio de Virología (LAVIR). Facultad de Ciencias Veterinarias. Universidad Nacional de La Plata, La Plata, Argentina
L. Fargnoli
Affiliation:
Consejo Nacional de Investigaciones Científicas y Técnicas, Mar del Plata, Buenos Aires, Argentina Laboratorio de Ecología de Enfermedades, Instituto de Ciencias Veterinarias del Litoral (ICIVET LITORAL), Universidad Nacional del Litoral, Santa Fe, Argentina
F. J. Reynaldi
Affiliation:
Consejo Nacional de Investigaciones Científicas y Técnicas, Mar del Plata, Buenos Aires, Argentina Laboratorio de Virología (LAVIR). Facultad de Ciencias Veterinarias. Universidad Nacional de La Plata, La Plata, Argentina
M. Eguaras
Affiliation:
Centro de Investigación en Abejas Sociales, Laboratorio de Artrópodos, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas, Mar del Plata, Buenos Aires, Argentina
M. Maggi
Affiliation:
Centro de Investigación en Abejas Sociales, Laboratorio de Artrópodos, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas, Mar del Plata, Buenos Aires, Argentina
*
Author for correspondence: Constanza Brasesco, Email: cobrasesco@gmail.com
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Abstract

Apis mellifera is infected by more than 24 virus species worldwide, mainly positive-sense, single-stranded RNA viruses of the Dicistroviridae and Iflaviridae families. Among the viruses that infect honey bees, Deformed wing virus is the most prevalent and is present as three master variants DWV-A, B, and C. Given that the ectoparasitic mite Varroa destructor vectors these virus variants, recombination events between them are expected, and variants and their recombinants can co-exist in mites and honeybees at the same time. In this study, we detect, through RT-qPCR, the presence of DWV-A and B in the same samples of adult bees from colonies of Argentina. Total RNA was extracted from pools of ten adult bees from 45 apiaries distributed across the main beekeeping Provinces of Argentina (Buenos Aires, Santa Fe, Córdoba, Santiago del Estero, Río Negro, and Mendoza); then RT-qPCR reactions were performed to detect DWV-A and B, with specific primer pairs. After the amplifications, PCR products (204 and 660 bp amplicons for DWV-B, and ~250 bp for DWV-A) were purified and sequenced to verify that they corresponded to reported sequences, analyzing them using the Blast software. Of the 45 samples analyzed by RT-qPCR, over 90% were infected with DWV-A and 47% were also positive for DWV-B, where it was found in high prevalence specifically in colonies of A. mellifera of the Buenos Aires Province. Future studies will determine the impact of this type of the virus and its ability to recombine with the other DWV types in the apiaries of our country.

Keywords

Apis melliferaDeformed wing virus variantsRT-qPCRVarroa destructor virus-1Varroa destructor
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 111 , Issue 1 , February 2021 , pp. 100 - 110
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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