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COI barcode based species-specific primers for identification of five species of stored-product pests from genus Cryptolestes (Coleoptera: Laemophloeidae)

Published online by Cambridge University Press:  22 January 2015

Z. Varadínová ,
Y. J. Wang ,
Z. Kučerová ,
V. Stejskal ,
G. Opit ,
Y. Cao ,
F. J. Li  and
Z. H. Li
Z. Varadínová
Affiliation:
Department of Entomology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, China Department of Zoology, Faculty of Science, Charles University in Prague, Viničná 7, Prague, Czech Republic
Y. J. Wang
Affiliation:
Department of Entomology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, China
Z. Kučerová
Affiliation:
Department of Pest Control of Stored Products and Food Safety, Crop Research Institute, Drnovská 507, Prague, Czech Republic
V. Stejskal
Affiliation:
Department of Pest Control of Stored Products and Food Safety, Crop Research Institute, Drnovská 507, Prague, Czech Republic
G. Opit
Affiliation:
Department of Entomology and Plant Pathology, Oklahoma State University, 127 Noble Research Center, Stillwater, Oklahoma, USA
Y. Cao
Affiliation:
Academy of State Administration of Grain, No. 11 Baiwanzhuang Street, Beijing, China
F. J. Li
Affiliation:
Academy of State Administration of Grain, No. 11 Baiwanzhuang Street, Beijing, China
Z. H. Li*
Affiliation:
Department of Entomology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, China
*
*Author for correspondence Phone: +86-10-62731299 Fax: +86-10-62733404 E-mail: lizh@cau.edu.cn
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Abstract

Flat grain beetles of the genus Cryptolestes (Coleoptera: Laemophloeidae) are one of the economically most important stored-product pests which feed on many kinds of agricultural products, especially grains. Nine of more than 40 described Cryptolestes species are recognized as stored-product pests and two of the pest species have a cosmopolitan distribution. Given the rapid growth in global trade of food products, ecological barriers to the spread of pests are easily overcome. Therefore, development of reliable systems for routine quarantine inspection and early infestation detection is vital. In the present study, we established a new rapid and accurate cytochrome c oxidase subunit I-based system for molecular identification of five common stored-product Cryptolestes species, namely, Cryptolestes capensis, Cryptolestes ferrugineus, Cryptolestes pusilloides, Cryptolestes pusillus and Cryptolestes turcicus. Five species-specific primer pairs for traditional uniplex polymerase chain reaction assay are described and their specificity and sensitivity for the identification process is evaluated using larval samples of 12 different populations from three continents (Asia, Europe and North America).

Keywords

COI barcode geneflat grain beetlesmolecular diagnosisspecies-specific primer
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 105 , Issue 2 , April 2015 , pp. 202 - 209
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Copyright
Copyright © Cambridge University Press 2015 

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References

Armstrong, K.F. & Ball, S.L. (2005) DNA barcodes for biosecurity: invasive species identification. Philosophical Transactions of the Royal Society B-Biological Sciences 360, 1813–1823.Google Scholar
Banks, H.J. (1979) Identification of stored product Cryptolestes spp. (Coleoptera, Cucujidae) – rapid technique for preparation of suitable mounts. Journal of the Australian Entomological Society 18, 217–222.CrossRefGoogle Scholar
deWaard, J.R., Mitchell, A., Keena, M.A., Gopurenko, D., Boykin, L.M., Armstrong, K.F., Pogue, M.G., Lima, J., Floyd, R., Hanner, R.H. & Humble, L.M. (2010) Towards a global barcode library for Lymantria (Lepidoptera: Lymantriinae) tussock moths of biosecurity concern. PLoS ONE 5, e14280.CrossRefGoogle ScholarPubMed
Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3, 294–299.Google Scholar
Halstead, D.G.H. (1993) Keys for the identification of beetles associated with stored products. 2. Laemophloeidae, Passandridae and Silvanidae. Journal of Stored Products Research 29, 99–197.Google Scholar
Howe, R.W. & Lefkowitch, L.P. (1957) The distribution of the storage species of Cryptolestes (Col., Cucujidae). Bulletin of Entomological Research 48, 795–809.Google Scholar
Jiang, F., Li, Z.H., Deng, Y.L., Wu, J.J., Liu, R.S. & Buahom, N. (2013) Rapid diagnosis of the economically important fruit fly, Bactrocera correcta (Diptera: Tephritidae) based on a species-specific barcoding cytochrome oxidase I marker. Bulletin of Entomological Research 103, 363–371.Google Scholar
Jinbo, U., Kato, T. & Ito, M. (2011) Current progress in DNA barcoding and future implications for entomology. Entomological Science 14, 107–124.CrossRefGoogle Scholar
Kučerová, Z. & Stejskal, V. (2002) Comparative egg morphology of silvanid and laemophloeid beetles (Coleoptera) occurring in stored products. Journal of Stored Products Research 38, 219–227.CrossRefGoogle Scholar
Lefkovitch, L.P. (1957) The biology of Cryptolestes ugandae Steel & Howe (Coleoptera, Cucujidae), a pest of stored products in Africa. Proceedings of the Zoological Society of London 128, 419–430.Google Scholar
Lefkovitch, L.P. (1962) A revision of African Laemophloeinae (Coleoptera: Cucujidae). Bulletin of the British Museum of Natural History (Entomology) 12, 167–245.Google Scholar
Lefkovitch, L.P. (1965) Differences between six species of Cryptolestes (Coleoptera, Cucujidae) in susceptibility to methyl bromide vapour. Bulletin of Entomological Research 56, 197–200.Google Scholar
Meyer, C.P. & Paulay, G. (2005) DNA barcoding: error rates based on comprehensive sampling. PLoS Biology 3, 2229–2238.Google Scholar
Morag, N., Saroya, Y., Braverman, Y., Klement, E. & Gottlieb, Y. (2012) Molecular identification, phylogenetic status, and geographic distribution of Culicoides oxystoma (Diptera: Ceratopogonidae) in Israel. PLoS ONE 7(3), e33610.Google Scholar
Nayak, M.K., Holloway, J.C., Emery, R.N., Pavic, H., Bartlet, J. & Collins, P.J. (2013) Strong resistance to phosphine in the rusty grain beetle, Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae): its characterisation, a rapid assay for diagnosis and its distribution in Australia. Pest Management Science 69, 48–53.Google Scholar
Nelson, L.A., Wallman, J.F. & Dowton, M. (2007) Using COI barcodes to identify forensically and medically important blowflies. Medical and Veterinary Entomology 21, 44–52.Google Scholar
Nowaczyk, K., Obrepalska-Steplowska, A., Gawlak, M., Throne, J.E., Olejarski, P. & Nawrot, J. (2009) Molecular techniques for detection of Tribolium confusum infestations in stored products. Journal of Economic Entomology 102, 1691–1695.Google Scholar
Obrepalska-Steplowska, A., Nowaczyk, K., Holysz, M., Gawlak, M. & Nawrot, J. (2008) Molecular techniques for the detection of granary weevil (Sitophilus granarius L.) in wheat and flour. Food Additives and Contaminants: Part A 25, 1179–1188.Google Scholar
Rees, D. (2004) Insects of Stored Products. CSIRO Publishing, Collingwood, Victoria, Australia, 192pp.Google Scholar
Thomas, M.C. (2005) New distribution records for two species of Cryptolestes Ganglbauer (Coleoptera: Laemophloeidae). Insecta Mundi 19(1–2), 88.Google Scholar
Thomas, M.C. & Zimmerman, M.L. (1989) A new species of stored products Cryptolestes from Thailand (Coleoptera: Cucujidae: Laemophloeinae). Journal of Stored Products Research 25, 77–79.Google Scholar
Throne, J.E., Doehlert, D.C. & McMullen, M.S. (2002) Susceptibility of commercial oat cultivars to Cryptolestes pusillus and Oryzaephilus surinamensis . Journal of Stored Products Research 39, 213–223.Google Scholar
Trematerra, P., Stejskal, V. & Hubert, J. (2011) The monitoring of semolina contamination by insect fragments using the light filth method in an Italian mill. Food Control 22, 1021–1026.Google Scholar
Virgilio, M., Backeljau, T., Nevado, B. & de Meyer, M. (2010) Comparative performances of DNA barcoding across insect orders. BMC Bioinformatics 11, 206.CrossRefGoogle ScholarPubMed
Wang, G., Li, C., Guo, X., Xing, D., Dong, Y., Wang, Z., Zhang, Y., Liu, M., Zheng, Z., Zhang, H., Zhu, X., Wu, Z. & Zhao, T. (2012) Identifying the main mosquito species in China based on DNA barcoding. PLoS ONE 7, e47051.Google Scholar
Wang, Y.J., Li, Z.H., Zhang, S.F., Varadínová, Z., Jiang, F., Kučerová, Z., Stejskal, V., Opit, G., Cao, Y. & Li, F.J. (2014) DNA barcoding of five common stored-product pest species of genus Cryptolestes (Coleoptera: Laemophloeidae). Bulletin of Entomological Research 104, 671–678.Google ScholarPubMed
Waugh, J. (2007) DNA barcoding in animal species: progress, potential and pitfalls. BioEssays 29, 188–197.CrossRefGoogle ScholarPubMed
Wells, J. & Williams, D. (2007) Validation of a DNA-based method for identifying Chrysomyinae (Diptera: Calliphoridae) used in a death investigation. International Journal of Legal Medicine 121, 1–8.CrossRefGoogle Scholar
Wiemers, M. & Fiedler, K. (2007) Does the DNA barcoding gap exist? – a case study in blue butterflies (Lepidoptera: Lycaenidae). Frontiers in Zoology 4, 1–16.Google Scholar
Yang, Q.Q., Kučerová, Z., Li, Z.H., Kalinovic, I., Stejskal, V., Opit, G. & Cao, Y. (2012) Diagnosis of Liposcelis entomophila (Insecta: Psocodea: Liposcelididae) based on morphological characteristics and DNA barcodes. Journal of Stored Products Research 48, 120–125.Google Scholar
Yang, Q.Q., Zhao, S., Kučerová, Z., Stejskal, V., Opit, G., Qin, M., Cao, Y., Li, F.J. & Li, Z.H. (2013) Validation of the 16S rDNA and COI DNA barcoding technique for rapid molecular identification of stored product psocids (Insecta: Psocodea: Liposcelididae). Journal of Economic Entomology 106, 419–425.CrossRefGoogle ScholarPubMed