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Colonisation of Glossina morsitans morsitans Westw. (Diptera, Glossinidae) in the laboratory using in vitro feeding techniques

Published online by Cambridge University Press:  10 July 2009

A. R. Mews ,
H. Baumgartner ,
D. Luger  and
E. D. Offori
A. R. Mews
Affiliation:
International Atomic Energy Agency, P.O. Box 590, A-1011 Vienna, Austria
H. Baumgartner
Affiliation:
International Atomic Energy Agency, P.O. Box 590, A-1011 Vienna, Austria
D. Luger
Affiliation:
International Atomic Energy Agency, P.O. Box 590, A-1011 Vienna, Austria
E. D. Offori
Affiliation:
International Atomic Energy Agency, P.O. Box 590, A-1011 Vienna, Austria
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Abstract

Fresh defibrinated bovine blood was superior to either haemolysed or freeze-dried defibrinated bovine blood when fed in vitro to female Glossina morsitans morsitans Westw. The presence of adenosine-5′-triphosphate (ATP) or other additives did not improve reproductive performance. The flies were able to compensate for considerable dilution of the blood with both serum and saline. Both parental and F1 female size affected the weight of the F2 puparia produced. The feeding of females on rabbits' ears on one day and in vitro on five days per week prevented any deterioration in performance over successive generations compared with a purely in vitro regime. A colony was established using this technique and results over a 13-month period were compared with those from a colony fed without rabbit supplement and another fed on rabbits only. The reproductive performance of females in the all-membrane-fed colony deteriorated from generation to generation, while those in the rabbit supplement colony performed similarly to those of the rabbit-fed colony except that survival was better but fecundity was poorer and the puparia produced were smaller.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 65 , Issue 4 , February 1976 , pp. 631 - 642
Copyright
Copyright © Cambridge University Press 1976

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References

Azevedo, J. F. De, Pinhão, R., Santos, A. M. T. Dos & Ferreira, A. E. (1968). Studies carried out with the Glossina morsitans colony of Lisbon. I. Some aspects of the evolution of the Glossina morsitans colony of Lisbon.—An. Esc. nac. Saúde pública e Med. trop. 2, 19–42.Google ScholarPubMed
Azevedo, J. F. De, Santos, A. M. T. Dos, Pinhão, R. C. & Guerreiro, J. P. (1969). Problems and prospects offered by the tsetse artificial breeding.—An. Esc. nac. Saúde público e Med. trop. 3, 125–135.Google ScholarPubMed
Bursell, E. (1960). The effect of temperature on the consumption of fat during pupal development in Glossina.—Bull. ent. Res. 51, 583–598.CrossRefGoogle Scholar
Cockings, K. L. (1961). Laboratory maintenance of Glossina pallidipes.—Rep. E. Afr. Trypan. Res. Org. 1960, 32–33.Google Scholar
Curtis, C. F. & Jordan, A. M. (1970). Calculations of the productivity of Glossina austeni Newst. maintained on goats and on lop-eared rabbits.—Bull. ent. Res. 59, 651–658.CrossRefGoogle ScholarPubMed
Curtis, C. F. & Langley, P. A. (1972). Use of nitrogen and chilling in the production of radiation-induced sterility in the tsetse fly Glossina morsitans.—Entomologia exp. appl. 15, 360–376.CrossRefGoogle Scholar
Galun, R. & Margalit, J. (1969). Adenine nucleotides as feeding stimulants of the tsetse fly Glossina austeni Newst.—Nature, Lond. 222, 583–584.Google Scholar
Galun, R. & Rice, M. J. (1971). Role of blood platelets in haematophagy.—Nature: New Biol. 233, 110–111.Google ScholarPubMed
Glasgow, J. P. (1961). Selection for size in tsetse flies.—J. Anim. Ecol. 30, 87–94.CrossRefGoogle Scholar
Itard, J. & Maillot, L. (1970). Les élevages de Glossines à Maisons-Alfort (France).—In de Azevedo, J. F. (Ed.) CriaÇão da mosca tsé-tsé em laboratório e sua aplicação pratica. 1° symposium internacional 22 e 23 abril de 1969. 125–136. Lisbon, Junta de Investigações do Ultramar.Google Scholar
Jordan, A. M. & Curtis, C. F. (1968). Productivity of Glossina austeni Newst. maintained on lop-eared rabbits.—Bull. ent. Res. 58, 399–410.Google Scholar
Jordan, A. M. & Curtis, C. F. (1972). Productivity of Glossina morsitans morsitans Westwood maintained in the laboratory, with particular reference to the sterile-insect release method.—Bull. Wld Hlth Org. 46, 33–38.Google Scholar
Langley, P. A. (1968). The effect of host pregnancy on the reproductive capability of the tsetse fly, Glossina morsitans, in captivity.—J. Insect Physiol. 14, 121–133.Google Scholar
Langley, P. A. (1972). The role of physical and chemical stimuli in the development of in vitro feeding techniques for tsetse flies Glossina spp. (Dipt., Glossinidae).—Bull. ent. Res. 62, 215–228.Google Scholar
Langley, P. A. & Pimley, R. W. (1973). Influence of diet composition on feeding and water excretion by the tsetse fly Glossina morsitans.—J. Insect Physiol. 19, 1097–1109.CrossRefGoogle ScholarPubMed
Langley, P. A. & Pimley, R. W. (1975). Quantitative aspects of reproduction and larval nutrition in Glossina morsitans morsitans Westw. (Diptera, Glossinidae) fed in vitro.—Bull. ent. Res. 65, 129–142.CrossRefGoogle Scholar
Lester, H. M. O. & Lloyd, L. (1928). Notes on the process of digestion in tsetse-flies.—Bull. ent. Res. 19, 39–60.CrossRefGoogle Scholar
Mews, A. (1972). Supplementing the diet of membrane-fed G. morsitans by feeding on rabbits' ears once per week.—Trans. R. Soc. trop. Med. Hyg. 66, 306–307.Google ScholarPubMed
Mews, A. R., Baumgartner, H., Luger, D. & Offori, E. D. (1973). A preliminary report on the establishment of a G. morsitans orientalis Vanderplank colony fed on membranes with rabbit supplement.—Trans. R. Soc. trop. Med. Hyg. 67, 291–292.CrossRefGoogle Scholar
Mews, A. R., Offori, E., Baumgartner, H. & Luger, D. (1972). Techniques used at the IAEA laboratory for rearing the tsetse fly Glossina morsitans Westwood.—In International Scientific Committee for Trypanosomiasis Research. Thirteenth meeting, Lagos, 1971, 243–254.Google Scholar
Mews, A. & Ruhm, F. (1971). Maintenance of Glossina morsitans Westwood fed through an artificial membrane on defibrinated blood.—In Sterility principle for insect control or eradication. Proceedings of a symposium jointly organized by the IAEA and FAO, held in Athens, 14–18 September, 1970. 283–293. Vienna, IAEA (Proceedings series STI/PUB/265).Google Scholar
Moloo, S. K. (1975). Amino acid synthesis in Glossina morsitans.—Trans. R. Soc. trop. Med. Hyg. 69, 281.Google Scholar
Moloo, S. K., Langley, P. A. & Balogun, R. A. (1974). Amino acid synthesis from glucose-U-14C in Glossina morsitans.—J. Insect Physiol. 20, 1807–1813.Google Scholar
Nash, T. A. M., Jordan, A. M. & Trewern, M. (1971). Mass rearing of tsetse flies (Glossina spp.): recent advances.—In Sterility principle for insect control or eradication. Proceedings of a symposium jointly organized by the IAEA and FAO, held in Athens, 14–18 September, 1970. 99–110. Vienna, IAEA (Proceedings series STI/PUB/265).Google Scholar
Phelps, R. J. & Clarke, G. P. Y. (1974). Seasonal elimination of some size classes in males of Glossina morsitans morsitans Westw. (Diptera, Glossinidae).—Bull. ent. Res. 64, 313–324.CrossRefGoogle Scholar
Rogers, A. (1971). Preliminary observations on an apparatus for the artificial feeding of tsetse flies.—Ann. trop. Med. Parasit. 65, 409–413.Google Scholar
Roubaud, E. (1917). Histoire d'un élevage de Glossina morsitans à l'lnstitut Pasteur de Paris.—Bull. Soc. Path. exot. 10, 629–640.Google Scholar