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DNA markers for identifying biotypes B and Q of Bemisia tabaci (Hemiptera: Aleyrodidae) and studying population dynamics

Published online by Cambridge University Press:  09 March 2007

V. Khasdan ,
I. Levin ,
A. Rosner ,
S. Morin ,
S. Kontsedalov ,
L. Maslenin  and
A.R. Horowitz
V. Khasdan
Affiliation:
Department of Entomology, Agricultural Research Organization, Gilat Research Center, M.P. Negev, 85280, Israel
I. Levin
Affiliation:
The Volcani Center, Bet Dagan, 50250, Israel
A. Rosner
Affiliation:
The Volcani Center, Bet Dagan, 50250, Israel
S. Morin
Affiliation:
Department of Entomology, Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, 76100, Israel
S. Kontsedalov
Affiliation:
The Volcani Center, Bet Dagan, 50250, Israel
L. Maslenin
Affiliation:
The Volcani Center, Bet Dagan, 50250, Israel
A.R. Horowitz*
Affiliation:
Department of Entomology, Agricultural Research Organization, Gilat Research Center, M.P. Negev, 85280, Israel
*
*Fax: ++ 972-8-9926 485 E-mail: hrami@volcani.agri.gov.il
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Abstract

The two most widespread biotypes of Bemisia tabaci (Gennadius) in southern Europe and the Middle East are referred to as the B and Q-type, which are morphologically indistinguishable. In this study various DNA markers have been developed, applied and compared for studying genetic diversity and distribution of the two biotypes. For developing sequence characterized amplified regions (SCAR) and cleaved amplified polymorphic sequences (CAPS) techniques, single random amplified polymorphic DNA (RAPD) fragments of B and Q biotypes, respectively, were used. The CAPS were investigated on the basis of nuclear sodium channel and the mitochondrial cytochrome oxidase I genes (mtCOI) sequences. In general, complete agreement was found between the different markers used. Analysis of field samples collected in Israel for several years, using these markers, indicated that the percentage of the Q biotype tends to increase in field populations as time progresses. This may be attributed to the resistance of the Q biotype to neonicotinoids and pyriproxyfen and the susceptibility of the B biotype to these insecticides.

Keywords

whiteflybiotypesDNA markerspopulation dynamics
Type
Research Article
Information
Bulletin of Entomological Research , Volume 95 , Issue 6 , December 2005 , pp. 605 - 613
Copyright
Copyright © Cambridge University Press 2005

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