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Supercooling points of diapausing forest tent caterpillar (Lepidoptera: Lasiocampidae) eggs

  • Johnny A. Uelmen (a1), John G. Duman (a2), Richard L. Lindroth (a1), Ezra G. Schwartzberg (a1) (a3) and Kenneth F. Raffa (a1)...


Forest tent caterpillar (Malacosoma disstria Hübner; Lepidoptera: Lasiocampidae) is a widely distributed defoliator that undergoes intermittent outbreaks. It overwinters as pharate larvae within egg bands, is univoltine, and experiences low winter temperatures in its northern range. Little is known about how low temperatures affect winter survival and cold tolerances, their cold tolerance strategy, or how cold tolerances may vary over time and among populations. We evaluated supercooling points (SCPs) from four populations of M. disstria eggs collected along a 552 km latitudinal gradient from southern Wisconsin to northern Minnesota, United States of America. To test for potential effects of winter environment, we also administered three overwintering regimes (Madison, Wisconsin; Cloquet, Minnesota; Ely, Minnesota). Supercooling points were recorded in November, February, and March of 2011–2012. Supercooling points varied with maternal source (egg band), time of winter season, population source, and overwintering treatment. Means ranged from −26.8 °C (±0.5 °C) to −40.3 °C (±0.3 °C), accordingly. In a separate laboratory experiment, 89% of pharate larvae held at −20 °C (18.3 °C above coolest mean SCP) survived, but none held at −45 °C (6.7 °C below lowest mean SCP) survived. This relatively high degree of cold tolerance in its overwintering stage, due to freeze avoidance, may partially explain survival patterns and limits of overwintering M. disstria in northern populations.


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Addo-Bediako, A., Chown, S.L., and Gaston, K.J. 2000. Thermal tolerance, climatic variability and latitude. Proceedings of the Royal Society of London B, 267: 739745.
Ayres, M.P. and Lombardero, M.J. 2000. Assessing the consequences of global change for forest disturbance from herbivores and pathogens. The Science of the Total Environment, 262: 263286.
Bale, J.S. 1987. Insect cold hardiness: freezing and supercooling – an ecophysiological perspective. Journal of Insect Physiology, 33: 899908.
Bale, J.S. and Hayward, S.A.L. 2010. Insect overwintering in a changing climate. Journal of Experimental Biology, 213: 980994.
Blais, J.R., Prentice, R.M., Sippel, W.L., and Wallace, D.R. 1955. Effects of the weather on the forest tent caterpillar Malacosoma disstria Hbn., in central Canada in the spring of 1953. The Canadian Entomologist, 87: 18.
Brown, C.E. 1965. Mass transport of forest tent caterpillar moths, Malacosoma disstria Hübner, by a cold front. The Canadian Entomologist, 97: 10731075.
Clark, M.S. and Worland, M.R. 2008. How insects survive the cold: molecular mechanism – a review. Journal of Comparative Physiology B, 178: 917933.
Cooke, B.J. and Roland, J. 2003. The effect of winter temperature on forest tent caterpillar (Lepidoptera: Lasiocampidae) egg survival and population dynamics in northern climates. Environmental Entomology, 32: 299311.
Daniel, C.J. and Myers, J.H. 1995. Climate and outbreaks of the forest tent caterpillar. Ecography, 18: 353362.
Denlinger, D.L. 1991. Relationship between cold hardiness and diapause. In Insects at low temperature. Edited by R.E. Lee and D.L. Denlinger. Chapman & Hall, New York, New York, United States of America. Pp. 174198.
Denlinger, D.L. and Lee, R.E. 2010. Low temperature biology of insects. Cambridge University Press, New York, New York, United States of America.
Denlinger, D.L., Lee, R.E., Yocum, G.D., and Kukal, O. 1992. Role of chilling in the acquisition of cold tolerance and the capacitation to express stress proteins in diapausing pharate larvae of the gypsy moth, Lymantria dispar . Archives of Insect Biochemistry and Physiology, 21: 271280.
Doucet, D., Walker, V.K., and Qin, W. 2009. The bugs that came in from the cold: molecular adaptations to low temperature in insects. Cellular and Molecular Life Sciences, 66: 14011418.
Duman, J.G. 2001. Antifreeze and ice nucleator proteins in terrestrial arthropods. Annual Review of Physiology, 63: 327357.
Duman, J.G., Wu, D.W., Xu, L., Tursman, D., and Olsen, T.M. 1991. Adaptations of insects to subzero temperatures. Quarterly Review of Biology, 66: 387410.
Evenden, M.L., Whitehouse, C.M., and Jones, B.C. 2015. Resource allocation to flight in an outbreaking forest defoliator, the forest tent caterpillar, Malacosoma disstria . Environmental Entomology, 44: 835845.
Fitzgerald, T.D. 1995. The tent caterpillars. Cornell University Press, Ithaca, New York, United States of America.
Fullard, J.H. and Napoleone, N. 2001. Diel flight periodicity and the evolution of auditory defences in the Macrolepidoptera. Animal Behaviour, 62: 349628.
Han, E.N. and Bauce, E. 1993. Physiological changes and cold hardiness of spruce budworm larvae, Choristoneura fumiferana (Clem.), during pre-diapause and diapause development under laboratory conditions. The Canadian Entomologist, 125: 10431053.
Hanec, W. 1966. Cold-hardiness in the forest tent caterpillar, Malacosoma disstria Hübner (Lasiocampidae, Lepidoptera). Journal of Insect Physiology, 12: 14431449.
Intergovernmental Panel on Climate Change. 2014. Summary for Policymakers. In Climate change 2014: impacts, adaptation, and vulnerability. Edited by C.B. Field, V.R. Barros, D.J. Dokken, K.J. Mach, M.D. Mastrandrea, T.E. Bilir, et al., Cambridge University Press, Cambridge, United Kingdom. Available from [accessed 16 December 2015].
Jepsen, J.U., Kapari, L., Hagen, S.B., Schott, T., Vindstad, O.P.L., Nilssen, A.C., et al. 2011. Rapid northwards expansion of a forest insect pest attributed to spring phenology matching with sub-Arctic birch. Global Change Biology, 17: 20712083.
Lee, R.E. 2010. A primer on insect cold tolerance. In Low temperature biology of insects. Edited by D.L. Denlinger and R.E. Lee. Cambridge University Press, Cambridge, United Kingdom. Pp. 334.
Lee, R.E. and Denlinger, D.L. 2010. Rapid cold hardening: ecological significance and underpinning mechanisms. In Low temperature biology of insects. Edited by D.L. Denlinger and R.E. Lee. Cambridge University Press, Cambridge, United Kingdom. Pp. 3558.
Netherer, S. and Schopf, A. 2010. Potential effects of climate change on insect herbivores in European forests – general aspects of the pine processionary moth as specific example. Forest Ecology and Management, 259: 831838.
Parry, D., Goyer, R.A., and Lenhard, G.J. 2001. Macrogeographic clines in fecundity, reproductive allocation, and offspring size of the forest tent caterpillar Malacosoma disstria . Ecological Entomology, 26: 281291.
Price, D.T., Alfara, R.I., Brown, K.J., Flannigan, M.D., Fleming, R.A., Hogg, E.H., et al. 2013. Anticipating the consequences of climate change for Canada’s boreal forest ecosystems. Environmental Reviews, 21: 322365.
Rochefort, S., Berthiaume, R., Hebert, C., Charest, M., and Bauce, E. 2011. Effect of temperature and host tree on cold hardiness of hemlock looper eggs along a latitudinal gradient. Journal of Inset Physiology, 57: 751759.
Schiesari, L. and O’Connor, M.B. 2013. Diapause: delaying the developmental clock in response to a changing environment. Current Topics in Developmental Biology, 105: 213246.
Schwartzberg, E.G., Jamieson, M.A., Raffa, K.F., Reich, P.B., Montgomery, R.A., and Lindroth, R.L. 2014. Simulated climate warming alters phenological synchrony between an outbreak insect herbivore and host trees. Oecologia, 175: 10411049.
Sformo, T., McIntyre, J., Walters, K.R., Barnes, B.M., and Duman, J. 2011. Probability of freezing in the freeze-avoiding beetle larvae Cucujus clavipes puniceus (Coleoptera: Cucujidae) from interior Alaska. Journal of Insect Physiology, 57: 11701177.
Somme, L. 1964. Effects of glycerol on cold-hardiness in insects. Canadian Journal of Zoology, 42: 87101.
Somme, L. 1982. Supercooling and winter survival in terrestrial arthropods. Comparative Biochemistry and Physiology, 73: 519543.
Trudeau, M., Mauffette, Y., Rochefort, S., Han, E., and Bauce, E. 2010. Impacts of host tree on forest tent caterpillar performance and offspring overwintering mortality. Environmental Entomology, 39: 498504.
Turnock, W.J. and Bilodeau, R.J. 1984. Survival of pupae of Mamestra configurata (Lepidoptera: Noctuidae) and two of its parasites in untilled and tilled soil. The Canadian Entomologist, 116: 257267.
Turnock, W.J., Lamb, R.J, and Bodnaryk, R.P. 1983. Effects of cold stress during pupal diapause on the survival and development of Mamestra configurata (Lepidoptera: Noctuidae). Oecologica, 56: 185192.
Uelmen, J.A., Lindroth, R.L., Tobin, P.C., Reich, P.B., Schwartzberg, E.G., and Raffa, K.F. 2016. Population source, spring temperatures, and overwintering regime shift phenology of insect-plant interaction: implications for changing climate in the southern boreal forest. Forest Ecology and Management, 362: 241250.
Voituron, Y., Mouquet, N., de Mazancourt, C., and Clobert, J. 2002. To freeze or not to freeze? An evolutionary perspective on the cold-hardiness strategies of overwintering ecotherms. The American Naturalist, 160: 255270.
Wood, D.M., Parry, D., Yanai, R. D., and Pitel, N.E. 2010. Forest fragmentation and duration of forest tent caterpillar (Malacosoma disstria Hübner) outbreaks in northern hardwood forests. Forest Ecology and Management, 260: 11931197.
Zachariassen, K.E. 1985. Physiology of cold tolerance in insects. Physiological Reviews, 65: 799832.
Zachariassen, K.E. and Hammel, H.T. 1976. Nucleating agents in the haemolymph of insects tolerant to freezing. Nature, 262: 285287.

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Supercooling points of diapausing forest tent caterpillar (Lepidoptera: Lasiocampidae) eggs

  • Johnny A. Uelmen (a1), John G. Duman (a2), Richard L. Lindroth (a1), Ezra G. Schwartzberg (a1) (a3) and Kenneth F. Raffa (a1)...


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