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New species in the Lasioglossum petrellum species group identified through an integrative taxonomic approach

  • Jason J. Gibbs (a1)


By means of an integrative taxonomic approach using morphology and DNA barcodes, multiple cryptic species within Lasioglossum (Dialictus) petrellum (Cockerell) (Hymenoptera: Halictidae) were discovered. Analysis of DNA barcode sequence data from across the supposed range of L. petrellum revealed distinct genetic differences that correlate with patterns of morphological variation and geographical distribution. The most morphologically distinct pair of species had the smallest DNA-barcode gap. The taxonomic limits of L. petrellum are revised and four new species are described and illustrated: L. (D.) tuolumnensesp. nov., L. (D.) griswoldisp. nov.L. (D.) droegeisp. nov., and L. (D.) viridipetrellumsp. nov. A key to species of the “L. petrellum” group is provided.

Une méthodologie taxonomique intégrée basée sur la morphologie et les codes à barres d'ADN a permis la découverte de plusieurs espèces cryptiques chez Lasioglossum (Dialictus) petrellum (Cockerell) (Hymnenoptera: Halictidae). Une analyse des données des séquences des codes à barres d'ADN provenant de l'ensemble de l'aire de répartition présumée de L. petrellum a révélé l'existence de différences génétiques distinctes qui correspondent à des patrons de variation morphologique et de répartition géographique. La paire d'espèces qui est la plus distincte morphologiquement est celle qui a la plus faible distance entre les codes à barres d'ADN. Les limites taxonomiques de L. petrellum se retrouvent donc redéfinies avec la description et l'illustration de quatre nouvelles espèces, L. (D.) tuolumnensesp. nov., L. (D.) griswoldisp. nov., L. (D.) droegeisp. nov. et L. (D.) viridipetrellumsp. nov. Une clef permet l'identification des espèces du groupe de “L. petrellum”.

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Ascher, J.S. et al. 2009. Apoidea species guide [online]. Available from [accessed 9 July 2009].
Batra, S.W.T. 1966. The life cycle and behavior of the primitively social bee, Lasioglossum zephyrum (Halictidae). University of Kansas Science Bulletin, 46: 359423.
Blüthgen, P. 1931. Beiträge zur Kenntnis der Bienengattung Halictus Latr. III. Mitteilungen aus dem Zoologischen Museum in Berlin, 17: 319398.
Campbell, J.W., Hanula, J.L., and Waldrop, T.A. 2007. Effects of prescribed fire and fire surrogates on floral visiting insects of the blue ridge province in North Carolina. Biological Conservation, 134: 393404.
Carman, G.M., and Packer, L. 1997. A cryptic species allied to Halictus ligatus Say (Hymenopetera: Halictidae) deteced by allozyme electrophoresis. Journal of Kansas Entomological Society, 69: 168176.
Cockerell, T.D.A. 1903. New bees from southern California and other records. Bulletin of the Southern California Academy of Sciences, 2: 8494.
Cockerell, T.D.A. 1916. The bees of the Coronado Islands. The Canadian Entomologist, 48: 5458.
Coyne, J.A., and Orr, H.A. 2004. Speciation. Sinauer Associates Inc., Sunderland, Massachusetts.
Danforth, B.N., Conway, L., and Ji, S.Q. 2003. Phylogeny of eusocial Lasioglossum reveals multiple losses of eusociality within a primitively eusocial clade of bees (Hymenoptera: Halictidae). Systematic Biology, 52: 2336.
DeSalle, R., Egan, M.G., and Siddall, M. 2005. The unholy trinity: taxonomy, species delimitation, and DNA barcoding. Philosophical Transactions of the Royal Society of London Series B Biological Sciences, 360: 19051916.
Ebmer, A.W. 1976. Liste der mittelleuropäischen Halictus- und Lasioglossum-Arten. Linzer Biologische Beiträge, 8: 393405.
Eickwort, G.C. 1988. Distribution patterns and biology of West Indian sweat bees (Hymenoptera: Halictidae). In Zoogeography of Caribbean insects. Edited by Liebherr, J.K.. Cornell University Press, Ithaca, New York.
Eickwort, G.C., and Eickwort, K.R. 1971. Aspects of the biology of Costa Rican halictine bees, II. Dialictus umbripennis and adaptations of its caste structure to different climates. Journal of the Kansas Entomological Society, 44: 343373.
Engel, M.S. 2001. A monograph of the Baltic amber bees and evolution of the Apoidea (Hymenoptera). Bulletin of the American Museum of Natural History, 259: 1192.
Engel, M.S., Hinojosa-Díaz, I.A., and Yáñez-Ordóñez, O. 2007. The Augochlora-like Dialictus from Guatemala and southern México (Hymenoptera: Halictidae). Acta Zoológica Mexicana, 23: 125134.
Folmer, O., Black, M., Hoeh, W., Lutz, R., and Vrijenhoek., R.. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3: 294299.
Gibbs, J. in press. An aberrant bee of the species Lasioglossum (Dialictus) disparile (Cresson) with brief taxonomic notes on the species. Journal of the Kansas Entomological Society.
Gibbs, J. 2009. An integrative taxonomic approach reveals new (and old) species in the Lasioglossum (Dialictus) tegulare (Robertson) species group (Hymenoptera, Halictidae). Zootaxa, 2032: 138.
Goloboff, P.A., Farris, J.S., Källersjö, M., Oxelman, B.Ramírez, M.J., and Szumik, C.A. 2003 a. Improvements to resampling measures of group support. Cladistics, 19: 324332.
Goloboff, P.A., Farris, J.S., and Nixon, K. 2003 b. T.N.T.: tree analysis using new technology [online]. Available from [accessed 31 December 2008].
Hajibabaei, M., deWaard, J.R., Ivanova, N.V., Ratnasingham, S., Dooh, R.T., Kirk, S.L., Mackie, P., and Hebert, P.D.N. 2005. Critical factors for assembling a high volume of DNA barcodes. Philosophical Transactions of the Royal Society of London Series B Biological Sciences, 360: 19591967.
Hajibabaei, M., Janzen, D.H., Burns, J.M., Hallwachs, W., and Hebert, P.D.N. 2006. DNA barcodes distinguish species of tropical Lepidoptera. Proceedings of the National Academy of Sciences of the United States of America, 103: 968971.
Harris, R.A. 1979. A glossary of surface sculpturing. Occasional Papers in Entomology (Sacramento), 28: 131.
Hebert, P.D.N., and Gregory, T.R. 2005. The promise of DNA barcoding for taxonomy. Systematic Biology, 54: 852859.
Hebert, P.D.N., Penton, E.H., Burns, J.M., Janzen, D.H., and Hallwachs, W. 2004. Ten species in one: DNA barcoding reveals cryptic species in the Neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences of the United States of America, 101: 1481214817.
Huelsenbeck, J.P., and Ronquist, F. 2001. MrBayes: Bayesian inference of phylogenetic trees. Bioinformatics, 17: 754755.
Hurd, P.D. Jr., 1979. Superfamily Apoidea. In Catalog of Hymenoptera in America North of Mexico. Edited by Krombein, K.V., Hurd, P.D. Jr., Smith, D.R., and Burks, B.D.. Smithsonian Institution Press, Washington, D.C. pp. 17412209.
Ivanova, N., deWaard, J., and Hebert, P. 2006. An inexpensive, automation-friendly protocol for recovering high-quality DNA. Molecular Ecology Notes, 6: 9981002.
Kalhorn, K.D., Barrows, E.D., and LaBerge, W.E. 2003. Bee (Hymenoptera: Apoidea: Apiformes) diversity in an Appalachian shale barrens. Journal of the Kansas Entomological Society, 76: 455468.
Kuhlmann, M., Else, G.R., Dawson, A., and Quicke, D.L.J. 2008. Molecular, biogeographical and phenological evidence for the existence of three western European sibling species in the Colletes succinctus group (Hymenoptera: Apidae). Organisms Diversity & Evolution, 7: 155165.
MacKay, P.A., and Knerer, G. 1979. Seasonal occurrence and abundance in a community of wild bees from an old field habitat in southern Ontario. The Canadian Entomologist, 111: 367376.
Meusnier, I., Singer, G.A.C., Landry, J.-F., Hickey, D.A., Hebert, P.D.N., and Hajibabaei, M. 2008. A universal DNA mini-barcode for biodiversity analysis. BMC Genomics, 9: 214.
Michener, C.D. 1951. Superfamily Apoidea. In Hymenoptera of America north of Mexico. Edited by Muesebeck, C.F., Krombein, K.V., and Townes, H.K.. United States Department of Agriculture, Agriculture Monograph No. 2. United States Government Printing Office, Washington, D.C. pp. 10431255.
Michener, C.D. 1974. The social behavior of the bees. Belknap Press of Harvard University Press, Cambridge, Massachusetts.
Michener, C.D. 1990. Reproduction and castes in social halictine bees. In Social insects: an evolutionary approach to castes and reproduction. Edited by Engels, W.. Springer-Verlag, New York. pp. 77121.
Michener, C.D. 2007. The bees of the world. 2nd ed. Johns Hopkins University Press, Baltimore, Maryland.
Mitchell, T.B. 1960. Bees of the eastern United States. Vol. I. North Carolina Agricultural Experiment Station Technical Bulletin No. 141.
Miyanaga, R., Maeta, Y., and Sakagami, S.F. 1999. Geographical variation of sociality and size linked color patterns in Lasioglossum (Evylaeus) apristum (Vachal) in Japan (Hymenoptera, Halictidae). Insectes Sociaux, 46: 224232.
Moure, J.S., and Hurd, P.D. Jr., 1987. An annotated catalog of the halictid bees of the Western Hemisphere (Hymenoptera: Halictidae). Smithsonian Institution Press, Washingtion, D.C.
Murray, T.E., Fitzpatrick, Ú., Brown, M.J.F., and Paxton, R.J. 2008. Cryptic species diversity in a widespread bumble bee complex revealed using mitochondrial DNA RFLPs. Conservation Genetics, 9: 653666.
Packer, L. 1992. The social organisation of Lasioglossum (Dialictus) laevissimum in southern Alberta. Canadian Journal of Zoology, 70: 17671774.
Packer, L. 1993. Multiple-foundress associations in sweat bees. In Queen number and sociality in insects. Edited by Keller, L.. Oxford University Press, New York.
Packer, L. 1994. Lasioglossum (Dialictus) tenax (Sandhouse) (Hymenoptera, Halictidae) as a solitary sweat bee. Insectes Sociaux, 41: 309313.
Packer, L. 1997. The relevance of phylogenetic systematics to biology: examples from medicine and behavioural ecology. Mémoires du Muséum National d'Histoire Naturelle, 173: 1129.
Packer, L. 2008. Phylogeny and classification of the Xeromelissinae (Hymenoptera: Apoidea, Colletidae) with special emphasis on the genus Chilicola. Systematic Entomology, 33: 7296.
Packer, L., and Taylor, J.S. 1997. How many hidden species are there? An application of the phylogenetic species concept to genetic data for some comparatively well known bee “species”. The Canadian Entomologist, 129: 587594.
Packer, L., Gibbs, J., Sheffield, C., and Hanner, R. 2009. DNA barcoding and the mediocrity of morphology. Molecular Ecology Resources, 9(S1): 4250.
Page, T.J., Choy, S.C., and Hughes, J.M. 2005. The taxonomic feedback loop: symbiosis of morphology and molecules. Biology Letters, 1: 139142.
Pesenko, Y.A. 2007. Subgeneric classification of the Palaearctic bees of the genus Evylaeus Robertson (Hymenoptera: Halictidae). Zootaxa, 1500: 154.
Prendini, L. 2005. Comment on “Identifying spiders through DNA barcodes”. Canadian Journal of Zoology, 83: 498504.
Sandhouse, G.A. 1924. New North American species of bees belonging to the genus Halictus (Chloralictus). Proceedings of the United States National Museum, 65: 143.
Sheffield, C.S., Hebert, P.D.N., Kevan, P.G., and Packer, L. 2009. DNA barcoding a regional bee (Hymenoptera: Apoidea) fauna and its potential for ecological studies. Molecular Ecology Resources, 9(S1): 196207.
Skevington, J.H., Kehlmaier, C., and Ståhls, G. 2007. DNA barcoding: mixed results for big-headed flies (Diptera: Pipunculidae). Zootaxa, 1423: 126.
Smith, M., Rodriguez, J.J., Whitfield, J.B.Deans, A.R.Janzen, D.H., Hallwachs, W., and Hebert, P.D.N. 2008. Extreme diversity of tropical parasitoid wasps exposed by iterative integration of natural history, DNA barcoding, morphology, and collections. Proceedings of the National Academy of Sciences of the United States of America, 105: 1235912364.
Smith, M.A., Woodley, N., Hallwachs, W., Janzen, D.H., and Hebert, P.D.N. 2006. DNA barcodes reveal cryptic host-specificity within the presumed polyphagous members of a genus of parasitoid flies (Diptera: Tachinidae). Proceedings of the National Academy of Sciences of the United States of America, 103: 36573662.
Smith, M.A., Wood, D.M., Janzen, D.H., Hallwachs, W., and Hebert, P.D.N. 2007. DNA barcodes affirm that 16 species of apparently generalist tropical parasitoid flies (Diptera, Tachinidae) are not all generalists. Proceedings of the National Academy of Sciences of the United States of America, 104: 49674972.
Song, H., Buhay, J.E., Whiting, M.F., and Crandall, K.A. 2008. Many species in one: DNA barcoding overestimates the number of species when nuclear mitochondrial pseudogenes are coamplified. Proceedings of the National Academy of Sciences of the United States of America, 105: 1348613491.
Tao, N., Richardson, R., Bruno, W., and Kuiken, C. 2005. FindModel [online]. Available from [accessed 24 February 2009].
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., and Higgins, D.G. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25: 48764882.
Vaglia, T., Haxaire, J., Kitching, I.J., Meusnier, I., and Rougerie, R. 2008. Morphology and DNA barcoding reveal three cryptic species within the Xylophanes neoptolemus and loelia species-group (Lepidoptera: Sphingidae). Zootaxa, 1923: 1836.
Warncke, K. 1975. Beitrag zur Systematik und Verbreitung der Furchenbienen in der Turkei (Hymenoptera, Apoidea, Halictus). Polskie Pismo Entomologiczne, 45: 81128.
Wcislo, W.T. 1997. Invasion of nests of Lasioglossum imitatum by a social parasite, Paralictus asteris (Hymenoptera: Halictidae). Ethology, 103: 111.
Wheeler, W.M. 1928. The social insects: their origin and evolution. Kegan Paul, Trench, Trubner and Co., Ltd. London.
Witt, J.D., Threloff, S.D.L., and Hebert, P.D.N. 2006. DNA barcoding reveals extraordinary cryptic diversity in an amphipod genus: implications for desert spring conservation. Molecular Ecology, 15: 30733082.
Yanega, D. 1997. Demography and sociality in halictine bees (Hymenoptera: Halictidae). In The evolution of social behavior in insects and arachnids. Edited by Choe, J.C. and Crespi, B.J.. Cambridge University Press, Cambridge, United Kingdom. pp. 293315.


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